MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 5
·321
·
IF 5.4
Liu Shicong,Zhang Yuanyuan,Zhao Hongyang,Liu Junqiu,Hou Chunxi
A “turn-on” fluorescence sensor was developed for cholesterol detection on the basis of fluorescence resonance energy transfer (FRET) between the organic fluorophore 1-(4-(1,2,2-triphenylvinyl)phenyl)pyrene (TPE-Py) and a gold nanoparticle-β-cyclodextrin conjugate (AuNPs-β-CD). Mechanistically, the AuNPs-β-CD, an energy acceptor, was synthesized via Au–S bonding, and TPE-Py transferred energy to the AuNPs via FRET, resulting in fluorescence quenching of TPE-Py. In the presence of cholesterol, which has a stronger binding affinity for the β-CD cavity, TPE-Py was competitively displaced, enabling the recovery of the fluorescence signal. This sensor has high sensitivity for cholesterol, with a low detection limit of 66 nM and a linear range of 0.5–30 µM. Furthermore, the sensor was successfully used to determine the cholesterol content in food samples with satisfactory recoveries, revealing its potential for food analysis.
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Graphical abstract
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PubMed
高影响因子
Pharmaceuticals
·
2026
·Vol. 19
·期 3
·469
·
IF 5.7
Lili Li,Xiaoya Zhang,Guangyuan Tang,Jianxin Wu,Qing Huang
Background: Glabridin, a natural compound derived fromGlycyrrhiza glabraL., possesses skin-lightening effects. This study aims to further elucidate the depigmentation mechanism of glabridin by investigating its effects on melanogenesis and melanin transfer.Methods: We initially confirmed the anti-melanogenic effects of glabridin in MNT-1 human melanoma cells. Then, we investigated the mechanism of its anti-melanogenic effect by evaluating the protein expression of β-catenin and MITF via Western blot. To investigate melanin transfer, we compared glabridin’s efficacy with that of niacinamide, a recognized inhibitor of melanosome transfer and employed two complementary experimental models: (1) α-melanocyte-stimulating hormone (α-MSH)-stimulated MNT-1 cells to analyze dendrite formation, and (2) a UVB-irradiated co-culture system of MNT-1 cells and HaCaT keratinocytes to evaluate melanin transfer.Results: By measuring glabridin’s effects on melanin content, tyrosinase activity, and melanogenesis-related protein expression confirmed its inhibition of melanin synthesis. Further investigation demonstrated that glabridin suppresses melanogenesis by downregulating β-catenin and MITF, indicating inhibition of the Wnt/β-catenin pathway. Furthermore, in α-MSH-treated MNT-1 cells, both glabridin and niacinamide were found to suppress dendrite formation and elongation. In a UVB-exposed co-culture system, both glabridin and niacinamide inhibited melanin transfer to keratinocytes. Mechanistically, these effects were linked to the regulation of Rho GTPases (Rac1, RhoA, Cdc42) and suppression of F-actin reorganization.Conclusions: This study provides, for the first time, evidence that the skin-lightening effect of glabridin involves two complementary mechanisms: inhibition of melanogenesis through suppression of the Wnt/β-catenin pathway, and attenuation of both dendricity and melanin transfer via the influence of Rho family GTPases expression.
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DOI
PubMed
高影响因子
Journal of Translational Medicine
·
2026
·Vol. 24
·期 1
·539
·
IF 9.7
Gao Yuan-Yuan,Sun Wen-Shuang,Sun Zi-Ying,Wang Jun-Rui,Lv Zhong-Yang,Li Yu-Jia,Tian Hao-Yuan,Bao Zheng-Yang,Qiu Xin-Ran,Wang Zheng,Zheng Shi-Zhong,Shao Jiang-Juan,Zhang Zi-Li,Meng Jia
Background Tendinopathy is a prevalent orthopaedic condition characterized by disrupted tendon homeostasis, with oxidative stress being a key contributing mechanism. Although the natural compound 18-β-Glycyrrhetinic acid (GA) exhibits antioxidant properties and is a therapeutic candidate for tendinopathy, its precise molecular mechanism remains unclear. This study aimed to elucidate how GA alleviates tendinopathy, with a focus on its role in regulating the HMGB1-cGAS-STING axis and NLRP3 inflammasome activation in the context of oxidative stress. Methods We employed single-cell RNA sequencing (scRNA-seq) of clinical samples, proteomics of animal tissues, and comprehensive pharmacological assays to investigate the mechanisms of tendinopathy. Furthermore, the rat tendinopathy model and H 2 O 2 -induced oxidative stress model of tendon stem cells (TSCs) were used to validate the protective effects of GA. Results We found that GA significantly reduced oxidative stress and subsequent inflammation, thereby mitigating collagen disruption in rats with tendinopathy. Notably, scRNA-seq revealed that the proportion of TSCs increased significantly during tendinopathy, which were particularly susceptible to reactive oxygen species (ROS). TSCs from oxidative damage and inhibited activation of the NLRP3 inflammasome by suppressing the cGAS-STING pathway. Mechanistically, GA promoted cGAS degradation by enhancing its interaction with the mitochondrial E3 ubiquitin ligase Mul1. This effect was mediated through high-mobility group box 1 (HMGB1), as GA disrupted the HMGB1-cGAS interaction. Specifically, GA induced methylation of HMGB1 at lysine 43, a modification essential for its activity. This methylation was catalyzed by the methyltransferase DOT1L, which was upregulated and directly bound by GA. Collectively, GA alleviates tendinopathy by targeting the DOT1L-HMGB1-cGAS axis to resolve oxidative stress and inflammation. Conclusion Collectively, our findings provide new insights into how oxidative stress accelerates tendinopathy progression. Moreover, they delineate the mechanism by which GA in mitigates oxidative damage and inflammation in TSCs by inhibiting the co-localization of HMGB1 and cGAS. Overall, this study offers scientific support for further developing GA as a promising therapeutic agent for tendinopathy treatment. Graphical The alternative text for this image may have been generated using AI.
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Stem Cell Research & Therapy
·
2026
·Vol. 17
·期 1
·200
·
IF 7.8
Chen Qi,Chen Xiaolong,Yang Qu,Miao Xinxin,Yuan Jinghong,Ding Shuihua,Ding Rui,Cai Shenghao,Li Bin,Cheng Xigao
Background Intervertebral disc degeneration (IVDD) is characterized by progressive nucleus pulposus cell loss and extracellular matrix degradation, in which persistent oxidative stress plays a critical pathogenic role. Transplantation of nucleus pulposus–derived stem cells (NPSCs) is a promising therapeutic strategy, yet the hostile oxidative microenvironment severely compromises cell survival. Although cellular quiescence has been suggested to enhance stress tolerance, its regulatory mechanisms and relevance in NPSCs remain largely unexplored. Methods Oxidative stress was evaluated in human degenerated disc tissues, a rat needle-puncture degeneration model, and tert-butyl hydroperoxide–treated NPSCs in vitro. Proliferating and quiescent NPSCs were compared for reactive oxygen species (ROS) levels, apoptosis, viability, and transcriptomic profiles. Pathway enrichment analyses were performed to identify critical signaling mechanisms. Recombinant transforming growth factor-beta 3 (rhTGF-β3) was used to activate the pathway, while small interfering RNA targeting the transforming growth factor-beta receptor type 2 ( Tgfbr2 ) and the pharmacological inhibitor SB431542 were applied for pathway suppression. Functional assays, organ culture, and in vivo transplantation were conducted to assess cell survival and regenerative effects. Results Elevated oxidative stress was consistently observed across clinical, animal, and cellular models of disc degeneration. Quiescent NPSCs demonstrated enhanced resistance to oxidative injury, with reduced ROS accumulation, decreased apoptosis, and improved survival. Transcriptomic analyses revealed suppression of metabolic and P53-mediated apoptotic pathways, alongside marked activation of TGF-β/SMAD signaling. Activation of this pathway induced quiescence, reduced ROS levels, inhibited mitochondrial apoptotic signaling, and protected NPSCs from oxidative injury, whereas pathway inhibition abolished these protective effects. In both organ culture and in vivo transplantation models, quiescent and TGF-β–activated NPSCs exhibited superior survival and significantly improved disc structural preservation compared with proliferating or pathway-blocked cells. Conclusion Activation of the TGF-β/SMAD pathway induces NPSC quiescence and enhances oxidative stress tolerance by suppressing P53-dependent mitochondrial apoptosis. Pharmacological induction of quiescence represents a potential strategy to improve stem cell–based therapies for intervertebral disc degeneration.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Yumeng Zhang,Yuanqi Lv,Lin Chen,Haizhou Wu,Yingyang Zhang,Zhiyao Suo,Shuxin Wang,Yuxin Liang,Xinglian Xu,Guanghong Zhou,Xianchao Feng
Nowadays, natural antioxidants abundant in polyphenols have been widely used to substitute synthetic antioxidants in meat products. In general, high doses of natural antioxidants are required to provide comparative antioxidant effects as synthetic antioxidants. Noticeably, the qualities of meat products can be jeopardized due to interactions between polyphenols and myofibrillar proteins (MPs). In this study, methyl-β-cyclodextrin was used to increase the polyphenol loading amount by preventing interactions between polyphenols and proteins. Solubility, electrophoresis, fluorescence spectroscopy, and surface hydrophobicity analyses indicated that methyl-β-cyclodextrin could dose-dependently inhibit epigallocatechin-3-gallate-induced attacks on MPs under oxidative stress. Gel strength, cooking loss, confocal laser scanning microscopy, dynamic rheological testing, and Raman spectrum during gelation were further analyzed to investigate the effects of methyl-β-cyclodextrin on the qualities of epigallocatechin-3-gallate-treated emulsion gel. Methyl-β-cyclodextrin addition prevented modification of the secondary structure of MPs caused by epigallocatechin-3-gallate. In consequence, the gel and emulsifying properties of MPs were significantly improved. Moreover, β-cyclodextrins could partly inhibit oxidative attacks on MPs and thus increase their solubility. These results indicated that methyl-β-cyclodextrin addition effectively enhanced epigallocatechin-3-gallate loading capacity in meat products.
研究领域:
食品科学与技术
·
蛋白质化学
·
多酚-蛋白质相互作用
·
氧化稳定性
·
功能食品
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Ben Chen,Tingting Lin,Haibo You,Liqun Fang,Chu Chu,Jintao Yang,Shengqiang Tong
The surface of SiO 2 coated magnetic nanoparticles (NMPs) was modified by bonding with sulfobutylether- β -cyclodextrin (SBE- β -CD) via carbodiimide activation for the first time. The magnetic nanoparticles were synthesized by chemical coprecipitation method under alkaline condition, and the magnetic particles were modified by citric acid . Then the surface of obtained magnetic nanoparticles was coated with SiO 2, and SBE- β -CD modified by citric acid was bonded to the surface of Fe 3 O 4 /SiO 2 MNPs under carbodiimide activation method. The synthesized functionalized magnetic nanoparticles were characterized by X-ray Diffraction, Fourier Transform Infra-Red spectroscopy, thermogravimetric analyses and transmission electron microscopy . These functional nano-sized particles were scrutinized for enantioselective liquid-liquid extraction of five racemic drugs/intermediates, including trans -paroxol, N-methyl duloxetine , fluoxetine , tropic acid and acetyltropic acid, and it was found that compared with traditional SBE- β -CD, the present magnetic silica nanoparticles bonded with SBE- β -CD showed higher enantiorecognition with some racemates using enantioselective liquid-liquid extractions. To our knowledge, this is the first-time report on preparation and application of magnetic nanoparticles bonded with SBE- β -CD in enantioselective liquid-liquid extraction for chiral separations .
研究领域:
纳米材料
·
分析化学
·
手性分离
·
磁性材料
·
超分子化学
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高影响因子
DALTON TRANSACTIONS
·
2015
·Vol. 44
·期 37
·16528-16533
·
IF 4.197
Z. C. Liu,W. P. Zhu,Y. H. Chen,Y. X. Li,Y. J. Ding,W. J. Yang,K. Li
In this paper, a simple small molecule (L) based on 4-(diethylamino)-2-hydroxy-benzaldehyde and carbohydrazide has been synthesized and characterized. Moreover, under ultrasonic conditions, a host–guest system with β-cyclodextrin and L was obtained. According to the hybridization process, the host–guest system showed excellent water solubility. The investigation of the fluorescence spectra revealed that the host–guest system exhibited a characteristic fluorescence behavior toward Al3+ in a pure water environment. Upon addition of Al3+, the host–guest system showed a strong blue fluorescence, which resulted from the fluorophore of L after the coordination of β-CD–L and Al3+ with a high binding constant (k = 3.1626 × 1011 M−1). In addition, SEM images demonstrated that the host–guest system expressed good crystallization behavior. Fluorescence microscope images of onion epidermal cells with β-CD–L–Al3+ proved that the water-soluble host–guest system possessed a high ability for cell permeability.
研究领域:
分析化学
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超分子化学
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荧光传感
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环境监测
·
生物成像
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高影响因子
FOOD CONTROL
·
2026
·
IF 7
Jin Deng,Sijia Wang,Nan Ma,Lu Dong,Jing-Min Liu,Yan Zhang,Yingshuang Lu,Shuo Wang
This study investigated the effect of oxygen concentration during oven cooking on flavor development, sensory quality, and β-carboline heterocyclic amines formation in beef patties. Samples were cooked at 190 °C (baking) and 210 °C (roasting) under controlled oxygen atmospheres of 5% and 21%. Key physicochemical parameters, including moisture content, color, volatile compounds, and free amino acids, were determined, and sensory evaluation was conducted. Both Harman and Norharman increased with temperature and oxygen availability. Harman increased from 0.08 to 0.21 ng/g at 190 °C and from 0.17 to 0.25 ng/g at 210 °C as oxygen elevated from 5% to 21%, while Norharman increased from 0.26 to 0.38 ng/g and from 0.46 to 0.53 ng/g, respectively. The two combined β-carboline heterocyclic amines content increased consistently from 0.41 to 0.91 ng/g across treatments. Reduced-oxygen cooking effectively suppressed lipid oxidation–derived aldehydes, including (E)-non-2-enal, and limited β-carboline formation without compromising sensory acceptance. Meanwhile, key aroma-active compounds such as nonanal and dimethyl trisulfide remained relatively stable, indicating that desirable meaty aroma was largely preserved. Notably, baking at 190 °C under 5% oxygen provided the optimal balance between desirable aroma retention and chemical safety. These findings demonstrate that oxygen-controlled oven cooking is a promising strategy to improve the sensory quality and safety of thermally processed meat products.
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高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xuezhi Sun,Zhibo Fu,Min Zhang,Hua Fu,Chuhui Lin,Jingjing Kuang,Hongyang Zhang,Ping Hu
Chirality is a universal feature of nature, and the effective differentiation of chiral molecules is still a challenge. Here we propose a method for recognizing chiral molecules based on an electrochemical detection method. We use β-cyclodextrin as an organic ligand and coordinate it with K ions to form a metal–organic framework (MOF), then we use diphenyl carbonate to connect MOFs to obtain chiral Cross-Linked MOF (CLMOF). This CLMOF is different from the cyclodextrin MOFs reported in previous literature in that it can maintain a stable structure in the aqueous environment. CLMOF and graphene oxide (GO) are jointly modified on the glassy carbon electrode (GCE) surface to build a GO-CLMOF/GCE electrochemical sensor which is used for the chiral recognition of the enantiomers of mandelic acid (MA). The DL enantiomers of MA exhibit different electrochemical behaviors on the GO-CLMOF/GCE sensor. Their peak current ratio (I L /I D ) reaches 1.8, and the peak potential difference ΔE (E D -E L ) is 96 mV. It shows good linearity in the range of 0.5–30 mM. The detection limits of D-MA and L-MA are 0.15 mM and 0.09 mM, respectively. The method was successfully applied to the detection of DL-MA enantiomer of unknown concentration in urine. The recognition of DL enantiomers on the electrode surface is possible because DL-MA forms diastereomers with CLMOF. The electrochemical sensor made of CLMOF and GO is simple to prepare and has excellent selectivity for DL enantiomers of MA, and provides a possible idea for the identification and quantification of chiral isomers.
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高影响因子
Nanoscale
·
2023
·Vol. 15
·期 8
·4063-4070
·
IF 6.7
Mengjiao Li,Qingjie Yu,Meie Zheng,Rongrong Jiang,Hongda Zhu,Huiling Guo,Hongmei Sun,Mingxing Liu
A label-free electrochemical immunosensor for high-sensitive detection of β-amyloid 1–42 (Aβ 1–42) was constructed based on Au-modified B, S, and N co-doped reduced graphene oxide (Au-BSN-rGO). The electronic structure of Au-BSN-rGO was investigated by first-principles calculations, which showed that the band gap of graphene was opened, thus improving its electrical conductivity. Moreover, Au-BSN-rGO was successfully prepared and characterized, and the obtained results discovered that it could be used as a signal amplifier for immunosensors due to the advantages of the good electrochemical characteristics and enormous surface area of BSN-rGO and the accelerated electron transfer ability of Au NPs. Furthermore, the label-free electrochemical immunosensor had a linear detection range of 0.1 pg mL−1–10 ng mL−1 and a detection limit of 0.072 pg mL−1, and it had good specificity, stability, and reproducibility. Also, this immunosensor showed recoveries of 89%–109% with an RSD of 2.61%–4.19% for detecting Aβ 1–42 in actual sample analysis. Therefore, the label-free electrochemical immunosensor based on Au-BSN-rGO should have a promising clinical application prospect for detecting Aβ 1–42.
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PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Hai-Yan Zhou,Qi Chen,Yi-Feng Zhang,Dou-Dou Chen,Xiao-Nan Yi,De-Shui Chen,Xin-Ping Cheng,Mian Li,Hong-Yan Wang,Kai-Qian Chen,Zhi-Qiang Liu,Yu-Guo Zheng
In order to improve the degradation activity of β-glucosidase (CpBgl) from Coniophora puteana , the structural modification was conducted. The enzyme activity of mutants CpBgl-Q20C and CpBgl-A240S was increased by 65.75% and 58.58%, respectively. These mutants exhibited maximum activity under the same conditions as wild-type CpBgl (65 ℃ and pH 5.0), slightly improved stabilities compared that of the wild-type, and remarkably enhanced activities in the presence of Mn 2+ or Fe 2+ . The V max of CpBgl-Q20C and CpBgl-A240S was increased to 138.18 and 125.14 μmol/mg/min, respectively, from 81.34 μmol/mg/min of the wild-type, and the catalysis efficiency ( k cat / K m ) of CpBgl-Q20C (335.79 min −1 /mM) and CpBgl-A240S (281.51 min −1 /mM) was significantly improved compared with that of the wild-type (149.12 min −1 /mM). When the mutant CpBgl-Q20C were used in the practical degradation of different biomasses, the glucose yields of filter paper, corncob residue, and fungi mycelia residue were increased by 17.68%, 25.10%, and 20.37%, respectively. The spatial locations of the mutation residues in the architecture of CpBgl and their unique roles in the enzyme-substrate binding and catalytic efficiency were probed in this work. These results laid a foundation for evolution of other glycoside hydrolases and the industrial bio-degradation of cellulosic biomass in nature.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Ziqi Zhao,Shaokun Liang,Chengjun Kang,Zhaoqiang Zhang,Liqin Zhou,Zhenxia Zhao,Xingzhen Qin,Yushan Pang,Hongbing Ji,Kungang Chai
Adsorption is considered as a promising method to purify the mixed acetophenone (AP) and 1-phenylethanol (1-PE) that are generally difficult to separate. Many efforts have been made to improve the preferential uptake of AP over 1-PE in aqueous phase, but there exists a practical bottleneck associated with their limited aqueous solubility. Herein, we demonstrate unprecedented reversal of selectivity in the organic medium enabled by β-ketoenamine-linked covalent organic frameworks (COFs), which were fabricated successfully by polycondensation between 1,3,5-triformylphloroglucinol (Tp) and diamines. The dependence of selective adsorption ability toward 1-PE on pore size was firstly investigated experimentally, indicating that TpBZ-COF with moderate pore size outperformed other analogues. The adsorption behaviors of 1-PE and AP onto TpBZ-COF were examined systematically. The evaluation results revealed a strong affinity toward 1-PE versus AP as well as excellent stability in recycling experiments. Further, the dynamic breakthrough experiments confirmed the practical application potential and remarkable recyclability. To probe the mechanism and structure–activity relationship, an imine-linked COF possessing similar porous structure was used as a reference for comparison of separation performance both experimentally and theoretically. The occurrence of hydrogen bonding between the hydroxyl of 1-PE and β-ketoenamine-linkage as H-bond acceptor in suitable pore spaces endows TpBZ-COF a fantastic candidate for purifying 1-PE and AP from petrochemical by-products.
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危险品化学品经营许可证（带存储）