PROCESS BIOCHEMISTRY
·
2025
·
IF 4
Yumei Feng,Yuanmin Zhu,Bohan Wang,Feifan Zhao,Yanxia Zhang,Xinqi Zhu,Huaixin Li,Longjiang Yu
Rapeseed meal hydrolysate (RSMH), is a low-cost, nitrogen-rich substrate with high potential for microbial fermentation. This study investigated the feasibility of using RSMH as an alternative nitrogen source to enhance docosahexaenoic acid (DHA) production in Schizochytrium sp. H016. Nutritional analysis revealed that RSMH contains abundant amino acids and phenolics, which are favorable for microbial growth and stress tolerance. Fermentation results showed that replacing yeast extract with RSMH significantly improved biomass accumulation, lipid production, and DHA yield. At 120 h, the maximum biomass, lipid, and DHA yields reached 45.80 g/L, 18.62 g/L, and 7.30 g/L, respectively, corresponding to increases of 23.96 %, 97.66 %, and 94.15 % compared to the control. RT-qPCR analysis revealed that RSMH upregulated key genes involved in glycolysis, fatty acid biosynthesis, triacylglycerol assembly, and antioxidant defense, which together promoted carbon flux toward lipid synthesis and improved oxidative stress resistance. Confocal microscopy further confirmed the increased accumulation of lipid droplets in RSMH medium. Fed-batch fermentation in a 5-L bioreactor yielded DHA and lipids of 15.51 and 33.81 g/L, respectively, with a final biomass of 63.80 g/L. These findings demonstrate that RSMH effectively substitutes yeast extract and upgrades rapeseed meal into a feedstock for DHA production.
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高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2017
·Vol. 183
·期 4
·1488-1502
·
IF 1.751
Wang Liming,Chen Yan
Cycloastragenol (CA), an exclusive telomerase activator, was derived from the Astragali Radix which is widely distributed in Turkey. Until now, there is no report to produce CA with effective and environment-friendly methods. Biotransformation is considered to be a promising technology. Thus, the present study was aimed to establish a biotransformation technology that could efficiently produce CA. In this paper, a microorganism, LG-502, was used to successfully transform astragaloside IV (ASI) to CA by analysis of thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The phylogenetic analysis of the 16S rRNA indicated that this strain belongs to Bacillus sp. Three metabolites were separated during the fermentation and characterized to be cyclogaleginoside B, CA, and 20 R , 24 S -epoxy-6 α , 16 β , 25-trihydroxy-9, 19-cycloartan-3-one based on NMR and MS spectroscopic analyses. The conversion rate of ASI and yield rate of CA were achieved as high as 89 and 84%, respectively, under optimized conditions. Enzymatic analysis showed that the glycosidases were mainly located inside the bacterial body, and the activities of glucosidases were much higher than the xylosidases under the experimental conditions. This study provides a feasible, effective, and eco-friendly way to prepare CA from ASI, which might greatly contribute to the applications of ASI.
研究领域:
药学
·
生物技术
·
微生物学
·
有机化学
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高影响因子
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
·
2025
·Vol. 75
·期 3
·006708
·
IF 2
Ling Yao,Manik Prabhu Narsing Rao,Cheng-Jie Xie,Peng Gao,Shu-Qun Zhang,Guo-Hong Liu,Shun-Gui Zhou
Two facultatively aerobic, Gram-stain-positive, rod-shaped, endospore-forming, endophytic bacteria, designated SGZ-1009Tand SGZ-1014, were isolated from the plantPennisetumsp. Strain SGZ-1009Tgrew at 5–50 °C, pH 4.5–11.0 and tolerated up to 4.5% NaCl (w/v), while strain SGZ-1014 exhibited growth at a temperature range of 10–50 °C, pH range 4.5–11.0 and tolerated up to 4.5% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belong to the genusPaenibacillus, closely related to the reference type strain ofPaenibacillus wenxiniaeDSM 100576T(similarity of 97.0%). The genomic relatedness values for both strains, SGZ-1009Tand SGZ-1014, compared to their closest reference strainP. wenxiniaeDSM 100576T, were 81.6% for average nucleotide identity and 26.0% for digital DNA–DNA hybridization, suggesting strain SGZ-1009Trepresents a novel species. The genomic DNA G+C content of strain SGZ-1009Twas 46.4%. Both strains shared anteiso-C15:0(55.9%) as the predominant fatty acid, menaquinone-7 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. Based on these results, strains SGZ-1009Tand SGZ-1014 represent a novel species ofPaenibacillus, for which the namePaenibacillus campisp. nov. is proposed. The type strain is SGZ-1009T(=MCCC 1K08892T=GDMCC 1.4280T=KCTC 43677T=JCM 36670T).
研究领域:
微生物学
·
植物学
·
分子生物学
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高影响因子
CURRENT MICROBIOLOGY
·
2026
·Vol. 83
·期 5
·250
·
IF 2.8
Tang Huiling,Zheng Zhouqing,Chen Mingsheng,Chen Xiaohui,Yan Dongqiang,Li Hong,Tuo Li,Li Feina
A Gram-stain-positive, rod-shaped and aerobic actinobacterium, designated strain M2MS4P-6 T , was isolated from surface-sterilized bark of Sonneratia apetala . The isolate was able to grow at 15–40 °C (optimum, 30–37 °C), at pH 6–8 (optimum, pH 7) and in the presence of 0–11% (w/v) NaCl (optimum, 2%). Strain M2MS4P-6 T showed the high 16S rRNA gene sequence similarities (95.5–98.9%) with the members of the genus Actinotalea . Phylogenetic trees based on 16S rRNA gene sequences and up-to-date bacterial core genes consistently showed that strain M2MS4P-6 T clustered tightly with Actinotalea lenta GDMCC 1.3827 T and formed an independent evolutionary branch. Digital DNA-DNA hybridization (19.5–21.6%), average nucleotide identity (72.5–78.7%) and average amino acid identity (55.3–75.9%) values between strain M2MS4P-6 T and the Actinotalea species were lower than the thresholds for species delineation, indicating that strain M2MS4P-6 T was a new species of the genus Actinotalea . The genomic G + C content of strain M2MS4P-6 T was 72.9%. The major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified phospholipid and two unidentified lipids. The predominant respiratory quinone was MK-8(H 4 ), and the major fatty acids were C 16:0 , anteiso-C 15:0 and C 14:0 . Gene functional analysis revealed that strain M2MS4P-6 T harbored an effective osmoregulation system to adapt the mangrove environment. Based on polyphasic taxonomic data, strain M2MS4P-6 T is considered to represent a novel Actinotalea species, for which the name Actinotalea endophytica sp. nov. is proposed. The type strain is M2MS4P-6 T (= CGMCC 4.7527 T =KCTC 49194 T ).
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Processes
·
2025
·Vol. 13
·期 10
·3245
·
IF 2.8
Peng Peng,Shuanghu Fan,Meiting Xu,Liyuan Liu,Xiaolin Zhang,Zihan Feng,Haina Du,Zimeng Wang,Qiao Qin,Weiming Feng,Hongyan Liu,Jingjing Guo
Phthalic acid esters (PAEs) are important plasticizers that have led to the heavy pollution of farmland, which has aroused significant and widespread concern for soil health and food safety. Microbial degradation has been recognized as an efficient pathway for removing PAEs from the environment. In this study, the PAE-degrading strain FR1 was isolated from sewage and determined to belong toGlutamicibacter. This strain degraded PAEs efficiently under a wide range of conditions—10–50 °C, pH of 6.0–11.0, and 0–8% salinity—demonstrating its great potential in PAE bioremediation. Genome sequencing provided complete genomic information, showing that the strain comprises one chromosome (3,404,214 bp) and three plasmids (112,089 bp, 80,486 bp, and 40,002 bp). The chromosome harbors 3238 protein genes, of which the PAE hydrolase genesdphGB1andmphGB2have been cloned. The hydrolase DphGB1 from hydrolase family I contained the catalytic triad Ser75-Asp194-His221. After heterogeneous expression and purification, the recombinant protein DphGB1, of about 30 kDa, was obtained. This hydrolase showed strong hydrolytic ability toward DEHP. The protein MphGB2 could also hydrolyze MBP. The molecular docking revealed interaction between DphGB1 and DBP. The main hydrolases of strain FR1-degrading PAEs were functionally identified. These results will promote the elucidation of the catalytic mechanisms of PAE hydrolases and the application of strain FR1 in farmland soil remediation.
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APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2019
·Vol. 85
·期 12
·
IF 4.077
Xinyu Lu,Weiwei Wang,Lige Zhang,Haiyang Hu,Ping Xu,Tao Wei,Hongzhi Tang
N,N-Dimethylformamide (DMF) is one of the most common xenobiotic chemicals, and it can be easily emitted into the environment, where it causes harm to human beings. Herein, an efficient DMF-degrading strain, DM1, was isolated and identified as Methylobacterium sp. This strain can use DMF as the sole source of carbon and nitrogen. Whole-genome sequencing of strain DM1 revealed that it has a 5.66-Mbp chromosome and a 200-kbp megaplasmid. The plasmid pLVM1 specifically harbors the genes essential for the initial steps of DMF degradation, and the chromosome carries the genes facilitating subsequent methylotrophic metabolism. Through analysis of the transcriptome sequencing data, the complete mineralization pathway and redundant gene clusters of DMF degradation were elucidated. The dimethylformamidase (DMFase) gene was heterologously expressed, and DMFase was purified and characterized. Plasmid pLVM1 is catabolically crucial for DMF utilization, as evidenced by the phenotype identification of the plasmid-free strain. This study systematically elucidates the molecular mechanisms of DMF degradation by Methylobacterium.IMPORTANCE DMF is a hazardous pollutant that has been used in the chemical industry, pharmaceutical manufacturing, and agriculture. Biodegradation as a method for removing DMF has received increasing attention. Here, we identified an efficient DMF degrader, Methylobacterium sp. strain DM1, and characterized the complete DMF mineralization pathway and enzymatic properties of DMFase in this strain. This study provides insights into the molecular mechanisms and evolutionary advantage of DMF degradation facilitated by plasmid pLVM1 and redundant genes in strain DM1, suggesting the emergence of new ecotypes of Methylobacterium.
研究领域:
环境微生物学
·
生物化学
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BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2016
·Vol. 64
·期 6
·843-850
·
IF 1.429
Jianbin Ye,Zhan Zhang,Ji Yan,Hui Hao,Xiangzhen Liu,Zongcan Yang,Ke Ma,Xuepeng Yang,Duobin Mao,Hao Zhou
Phytosterols have been demonstrated to be precursors of polycyclic aromatic hydrocarbons (PAHs) formed during biomass pyrolysis. Here, a novel Paenibacillus sp. was evaluated for its ability to degrade phytosterols in tobacco waste extract (TWE). The optimal conditions for cell growth and stigmasterol (a representative of phytosterols) degradation were 37 °C, pH 7.0, 1.0 g/L yeast extract, and 6.0 g/L glucose. Paenibacillus sp. could degrade stigmasterol under high concentrations of glucose (up to 130 g/L) and tolerate wide pH (5.0–9.0) and temperature (25–42 °C) ranges. The new strain could degrade stigmasterol completely into CO 2 and H 2 O, and no intermediate steroids were detected during the degradation process. Phytosterol degradation in TWE was demonstrated by high-performance liquid chromatography–tandem mass spectrometry. Under optimal conditions (37 °C, pH 7.0, with the exponential-phase cells), the total degradation ratio of phytosterols reached 38.5% in TWE, including 45.2% of stigmasterol, 37.4% of β-sitosterol, 27.3% of campesterol, and 28.7% of cholesterol. These results showed that Paenibacillus sp. is a candidate for phytosterol degradation in TWE and other biomass and is potentially useful in reducing the PAHs generated from biomass pyrolysis.
研究领域:
环境微生物学
·
生物降解
·
生物质处理
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Dan Ma,Yang Lyu,Yujia Wang,Xiaochen Li,Junheng Wang,Yingying Wang,Sijun Dong
Phthalates (PAEs), such as dibutyl phthalate (DBP), are emerging contaminants in agriculture that are commonly used in plastic films, pesticides, and soil amendments. Their accumulation in water threatens ecosystems and human health. Biodegradation offers a promising solution, but the DBP degradation mechanisms remain unclear. This study identified two 4,5-PCD isoenzymes (PCD34 and PCD67) in Curvibacter sp. PAE-UM. PCD34 showed higher affinity and broader substrate specificity, while PCD67 had better thermal stability and metal ion sensitivity. Both enzymes converted protocatechuate (PCA) to 4CHMS at 45% efficiency in 5 min. Molecular dynamics revealed the stable binding of PCD67, while mutagenesis enhanced PCD34 activity (124.4%) by reducing steric hindrance. These findings may aid in the engineering of strains for PAE bioremediation in agricultural wastewater.
研究领域:
环境微生物学
·
生物技术
·
酶学
·
分子生物学
·
生态毒理学
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Environmental Technology & Innovation
·
2025
·
IF 7.1
Xiao Tan,Gang Pan,Wenxue Che,Ying Man,Liyi Wang,Jinting Li,Hui Zhao
Microalgal-bacterial consortia provide a sustainable, low-cost solution for antibiotic-contaminated wastewater remediation. Microalgae cultivation typically involves natural symbiotic bacteria, which are often overlooked. To fill this gap, this study evaluated the adaptive response of Dunaliella sp. and its natural symbiotic bacteria to sulfamethoxazole (SMX) through cultivation experiments and 16S rDNA sequencing. The results demonstrated strong SMX tolerance but limited removal efficiency by the consortium. The adaptive responses were characterized by increased bacterial diversity and connectivity, with quorum sensing bacteria-driven community changes and biofilm formation playing a critical role in this process. Dunaliella sp. exhibited strong antioxidant capacity (with catalase playing a key role) and increased extracellular polymeric substances secretion, which not only contributed to its tolerance but also promoted microalgae-bacteria interactions, enhancing consortium adaptability. These findings strengthen our understanding of the responses of natural symbiotic bacteria to antibiotics and advance the development of microalgae-bacteria symbiosis technology for wastewater treatment.
研究领域:
环境科学
·
微生物学
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高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Zehao Li,Wanying Wang,Guoguo Wang,Yingjie Feng,Yan Chen,Zhijian Li,Lulu Zhang,Jinchu Yang
Carvone has been widely used in pharmaceutical, food, cosmetic, and chemical industries due to its pleasant odor with high economic value and pharmacological activities. Carveol dehydrogenase is an enzyme that catalyzes the oxidation of carveol into carvone in the presence of NAD(P) + . In this study, two dehydrogenase genes from Klebsiella sp. O852 ( KlebADH1 and KlebADH2 ) were cloned and expressed in Escherichia coli BL21(DE3), and the purified enzymes were characterized for their biocatalytic properties. KlebADH1 and KlebADH2 displayed optimal catalytic activity at 40 °C and 30 °C, respectively, at pH 9. In addition to carveol, KlebADH1 had the potential to catalyze the oxidation of perillyl alcohol, nerol and geraniol, while borneol and geraniol could serve as potential substrates for KlebADH2. The substrate kinetic parameters of enzymes were performed by changing the concentration of carveol, and the results indicated that KlebADH2 exhibited a high affinity for carveol. Additionally, KlebADH1 and KlebADH2 were confirmed to possess carveol dehydrogenase activity through a double-enzyme-coupled (DEC) biotransformation system. The investigation of the biocatalytic properties of KlebADH1 and KlebADH2 laid a solid foundation for further study of carvone sustainable synthesis.
研究领域:
生物技术
·
酶学
·
化学工程
·
药学
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高影响因子
CURRENT MICROBIOLOGY
·
2020
·Vol. 78
·期 1
·383-388
·
IF 1.746
Zhou Jie,Chen Jianhao,Ma Jieyu,Xu Ning,Xin Fengxue,Zhang Wenming,Zhang Hao,Dong Weiliang,Jiang Min
A Gram-staining-negative, aerobic, non-motile, rod-shaped bacterium with degradation ability of chitin, designated strain YD-1 T , was isolated from landfill soil sample collected in Wenzhou, Zhejiang province, China. The growth of strain YD-1 T occurred optimally in the tryptone soy broth (TSB) with 1.0% NaCl at pH 7.0–8.0, 30 °C. Ubiquinone-8 (Q-8) was the predominant quinone. The polar lipids of strain YD-1 T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, five glycolipids and four lipids. The major fatty acids were iso-C 15:0 (30.7%), iso-C 17:1 ω9c (23.2%), iso-C 11:0 (18.9%), iso-C 11:0 3-OH (6.8%) and iso-C 17:0 (5.9%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain YD-1 T was affiliated to the genus Luteimonas with the highest similarity to Luteimonas marina KCTC 12327 T (97.3%), followed by Luteimonas aquatica DSM 22088 T (96.5%) and Luteimonas composti CCUG 53595 T (96.4%). The genomic DNA G + C content of strain YD-1 T was 71.8 mol%. Average nucleotide identity (ANI) and the digital DNA–DNA hybridizations (dDDH) for draft genomes between strain YD-1 T and Luteimonas marina KCTC 12327 T were 82.7% and 26.1%, respectively. On the basis of genotypic, phenotypic and chemotaxonomic data, strain YD-1 T is considered to represent a novel species to degrade chitin in the genus Luteimonas , for which the name Luteimonas wenzhouensis sp. nov. is proposed, with YD-1 T (= KCTC 72425 T = CCTCC AB 2019153 T ) as the type strain.
研究领域:
微生物学
·
环境科学
·
分子生物学
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2017
·
IF 6.065
Lan-jia Pan,Jie Li,Chun-xing Li,Xiao-da Tang,Guang-wei Yu,Yin Wang
Ciprofloxacin (CIP) is an antibiotic drug frequently detected in manure compost and is difficult to decompose at high temperatures, resulting in a potential threat to the environment. Microbial degradation is an effective and environmentally friendly method to degrade CIP. In this study, a thermophilic bacterium that can degrade CIP was isolated from sludge sampled from an antibiotics pharmaceutical factory. This strain is closely related to Thermus thermophilus based on 16S rRNA gene sequence analysis and is designated C419. The optimal temperature and pH values for CIP degradation are 70 °C and 6.5, respectively, and an appropriate sodium acetate concentration promotes CIP degradation. Seven major biodegradation metabolites were identified by an ultra-performance liquid chromatography tandem mass spectrometry analysis. In addition, strain C419 degraded other fluoroquinolones, including ofloxacin, norfloxacin and enrofloxacin. The supernatant from the C419 culture grown in fluoroquinolone-containing media showed attenuated antibacterial activity. These results indicate that strain C419 might be a new auxiliary bacterial resource for the biodegradation of fluoroquinolone residue in thermal environments.
研究领域:
环境微生物学
·
生物修复
·
药理学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）