ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Yu Ma,Wei Yang,Peiyao Liang,Ruzhen Feng,Tianming Qiu,Jingyuan Zhang,Xiance Sun,Qiujuan Li,Guang Yang,Xiaofeng Yao
As a persistent organic pollutant, perfluorooctane sulfonate (PFOS) has a serious detrimental impact on human health. It has been suggested that PFOS is associated with liver inflammation. However, the underlying mechanisms are still unclear. Here, PFOS was found to elevate the oligomerization tendency of voltage-dependent anion channel 1 (VDAC1) in the mice liver and human normal liver cells L-02. Inhibition of VDAC1 oligomerization alleviated PFOS-induced nucleotide-binding domain and leucine-rich repeat protein-3 (NLRP3) inflammasome activation. Cytoplasmic membrane VDAC1 translocated to mitochondria was also observed in response to PFOS. Therefore, the oligomerization of VDAC1 occurred mainly in the mitochondria. VDAC1 was found to interact with the ATP synthase beta subunit (ATP5B) under PFOS treatment. Knockdown of ATP5B or immobilization of ATP5B to the cytoplasmic membrane alleviated the increased VDAC1 oligomerization and NLRP3 inflammasome activation. Therefore, our results suggested that PFOS induced NLRP3 inflammasome activation through VDAC1 oligomerization, a process dependent on ATP5B to transfer VDAC1 from the plasma membrane to the mitochondria. The findings offer novel perspectives on the activation of the NLRP3 inflammasome, the regulatory mode on VDAC1 oligomerization, and the mechanism of PFOS toxicity.
研究领域:
毒理学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Xiaoxiao Su,Mengyao Wang,Hui Ouyang,Shijia Yang,Wenwen Wang,Yong He,Zhifeng Fu
A facile bioluminescent protocol was developed to detect the total amount of viable Gram-positive bacteria based on a novel antibiotic-affinity strategy. Vancomycin-functionalized magnetic particles were adopted to isolate Gram-positive bacteria from sample matrix, utilizing the five-point hydrogen bonds binding between vancomycin and D-Alanyl- d -Alanine moieties on the cell wall. Then the bioluminescence signal from the bacterial intracellular adenosine triphosphate was collected to quantify the captured bacteria after cells lysis. In this proof-of-principle work, the developed protocol was applied to detect four Gram-positive bacteria, including Staphylococcus aureus , Micrococcus luteus , Bacillus cereus and Streptococcus mutans . It showed a linear range of 1.0 × 10 2 –1.0 × 10 7 CFU mL −1 and a detection limit of 33 CFU mL −1 for the four model bacteria. The whole assay process could be completed within 70 min. Gram-negative bacteria and dead bacteria all showed negligible interference to the detection of the viable Gram-positive bacteria. The method was successfully applied to quantify the amount of viable Gram-positive bacteria in environmental, food and pharmaceutical samples with acceptable recovery values ranging from 72.0% to 120.0%. The protocol possessed numerous advantages such as high sensitivity, low cost, ideal specificity and short detection time. It could also be extended to Gram-negative bacteria detection by utilizing other antibiotics acting on Gram-negative bacteria.
研究领域:
微生物学
·
分析化学
✓ 已验证
DOI
高影响因子
SCIENCE
·
2024
·Vol. 383
·期 6689
·
IF 44.7
Wei Ying,Yaowei Wang,Hong Wei,Yongming Luo,Qian Ma,Heyuan Zhu,Hilde Janssens,Nemanja Vukašinović,Miroslav Kvasnica,Johan M. Winne,Yongxiang Gao,Shutang Tan,Jiří Friml,Xin Liu,Eugenia Russinova,Linfeng Sun
Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.
研究领域:
植物生物学
·
分子生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Fukui Shen,Wen Yang,Guoqing Luan,Jiamin Peng,Zhenqiang Li,Jie Gao,Yuanyuan Hou,Gang Bai
Background Catalpol (CAT), a naturally occurring iridoid glycoside sourced from the root of Rehmannia glutinosa , affects mitochondrial metabolic functions. However, the mechanism of action of CAT against pyrexia and its plausible targets remain to be fully elucidated. Purpose This study aimed to identify the specific targets of CAT for blocking mitochondrial thermogenesis and to unveil the unique biological mechanism of action of the orthogonal binding mode between the hemiacetal group and lysine residue on the target protein in vivo . Methods Lipopolysaccharide (LPS)/ carbonyl cyanide 3-chlorophenylhydrazone (CCCP)-induced fever models were established to evaluate the potential antipyretic effects of CAT. An alkenyl-modified CAT probe was designed to identify and capture potential targets. Binding capacity was tested using in-gel imaging and a cellular thermal shift assay. The underlying antipyretic mechanisms were explored using biochemical and molecular biological methods. Catalpolaglycone (CA) was coupled with protein profile identification and molecular docking analysis to evaluate and identify its binding mode to UCP2. Results After deglycation of CAT in vivo , the hemiacetal group in CA covalently binds to Lys239 of UCP2 in the mitochondria of the liver via an ɛ-amine nucleophilic addition. This irreversible binding affects proton leakage and improves mitochondrial membrane potential and ADP/ATP transformation efficiency, leading to an antipyretic effect. Conclusion Our findings highlight the potential role of CA in modulating UCP2 activity or function within the mitochondria and open new avenues for investigating the therapeutic effects of CA on mitochondrial homeostasis.
研究领域:
药理学
·
分子生物学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
FITOTERAPIA
·
2025
·
IF 2.6
Yao Wang,Pan Wang,Fangfang Xu,Han Zhou,Xiaomin Xie,Tao Hou,Fengbin Luo,Yuchang Sun,Yanfang Liu,Jixia Wang,Dongmei Fu
ATP citrate lyase (ACLY) is a crucial enzyme in glycolipid metabolism. To date, only its one prodrug bempedoic acid has been approved. The discovery of direct ACLY inhibitors is important for developing new lipid-lowering drugs. Grape seeds, used in functional foods and medicines, have lipid-lowering efficacy. However, it remains unknown whether their components directly inhibit ACLY. To investigate it, a multi-component screening method was used and proanthocyanidin B2 was identified as a novel ACLY inhibitor with an IC 50 value of 2.73 μM. Differential scanning fluorimetry confirmed its directly binding to ACLY. Proanthocyanidin B2 acted as a noncompetitive inhibitor in kinetic studies and bound stably with ACLY in molecular dynamics simulations. In vitro , proanthocyanidin B2 reduced lipid accumulation via inhibiting key transcription factors associated with ACLY. This study revealed a novel ACLY inhibitor contributing to lipid-lowering efficacy of grape seeds and provided a unique scaffold for ACLY drug design.
研究领域:
生物化学
·
药理学
·
分子生物学
·
天然产物化学
·
代谢疾病
·
药物发现
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PROTEOME RESEARCH
·
2016
·
IF 4.173
Zhilei Liu,Yadong Hu,Haisha Liang,Zhongyuan Sun,Shan Feng,Haiteng Deng
PRDX3 is a mitochondrial peroxide reductase that regulates cellular redox state. It has been reported that PRDX3 is overexpressed in liver cancer, but how PRDX3 is involved in hepatocellular carcinoma (HCC) tumorigenesis and progression has not been well-characterized. In the present study, we established two stable cell lines by overexpressing or knocking down PRDX3 in HepG2 cells. We found that PRDX3 silencing decreased the growth rate of HepG2 cells and increased mtDNA oxidation. Quantitative proteomics identified 475 differentially expressed proteins between the PRDX3 knockdown and the control cells. These proteins were involved in antioxidant activity, angiogenesis, cell adhesion, cell growth, ATP synthesis, nucleic acid binding, redox, and chaperones. PRDX3 knockdown led to the down-regulation of ATP synthases and the decreased cellular ATP level, contributing to the slow-down of cell growth. Furthermore, silencing PRDX3 enhanced invasive properties of HepG2 cells via TIMP-1 down-regulation and the increased ECM degradation. Taken together, our results indicate that PRDX3 promotes HCC growth and mediates cell migration and invasiveness and is a potential therapeutic target for HCC treatment.
研究领域:
肿瘤学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Yong-Ping Dong,Ying Zhou,Jiao Wang,Jun-Jie Zhu
In the present work, strong anodic electrogenerated chemiluminescence (ECL) of Ru(bpy)32+ was observed at a graphene oxide modified glassy carbon electrode (GO/GCE) in the absence of coreactants. The electrocatalytical effect of GO on the oxidation of Ru(bpy)32+ suggested that GO itself can act as the coreactant of Ru(bpy)32+ ECL, which can be used to fabricate the ECL biosensor. Thiol group terminated adenosine triphosphate (ATP) aptamer was immobilized on the GO film via DNA hybridization. When gold nanoparticles/graphene oxide (AuNPs/GO) nanocomposites were modified on the aptamer through the S–Au bond to form a sandwich-like structure, the ECL resonance energy transfer (ECL-RET) could occur between Ru(bpy)32+ and AuNPs/GO nanocomposites, resulting in an apparent decrease of ECL signal. After the ECL sensor was incubated in ATP solution, the AuNPs/GO nanocomposites were released from the electrode due to the specific interaction between aptamer and ATP, leading to the increased ECL signal. On the basis of these results, an ECL aptasensor was fabricated and could be used in the sensitive and selective detection of ATP in the range of 0.02–200 pM with a detection limit of 6.7 fM (S/N = 3). The proposed ECL aptasensor can be applied in the detection of ATP in real samples with satisfactory results.
研究领域:
分析化学
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 22
·3931
·
IF 5.719
Weikai Sun,Hongxiang Ou,Ziwei Chen
Adsorption is one of the effective methods of treating dye wastewater. However, the selection of suitable adsorbent materials is the key to treating dye wastewater. In this paper, GO−ATP was prepared by an intercalation method by inserting graphene oxide (GO) into the interlayer of alabaster attapulgite (ATP), and GO−ATP@CS−PVA aerogel was prepared by co−blending−crosslinking with chitosan (CS) and polyvinyl alcohol (PVA) for the adsorption and removal of crystalline violet dye from the solution. The physicochemical properties of the materials are characterized by various methods. The results showed that the layer spacing of the GO−ATP increased from 1.063 nm to 1.185 nm for the ATP, and the specific surface area was 187.65 m2·g−1, which was 45.7% greater than that of the ATP. The FTIR results further confirmed the success of the GO−ATP intercalation modification. The thermogravimetric analysis (TGA) results show that the aerogel has good thermal stability properties. The results of static adsorption experiments show that at 302 K and pH 9.0, the adsorption capacity of the GO−ATP@CS−PVA aerogel is 136.06 mg·g−1. The mass of the aerogel after adsorption−solution equilibrium is 11.4 times that of the initial mass, with excellent adsorption capacity. The quasi−secondary kinetic, Freundlich, and Temkin isotherm models can better describe the adsorption process of the aerogel. The biobased composite aerogel GO−ATP@CS−PVA has good swelling properties, a large specific surface area, easy collection and a low preparation cost. The good network structure gives it unique resilience. The incorporation of clay as a nano−filler can also improve the mechanical properties of the composite aerogel.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Ru-Ru Gao,Wei Dong
A new strategy was put forward to fabricate amorphous coordination polymer nanoparticles (CPNs) self-assembled by lanthanide ions (Ln 3+ ) and ATP whose adenine subunit and phosphate group served as bidentate ligands for Ln 3+ . These ATP/Ln 3+ CPNs showed weak luminescence but could be sensitized by introduction of external stimuli. Hence, they could be “artificial tongues” to respond to multifarious analytes (pyridine carboxylic acids or metal ions). Inspired the fact that both ATP/Tb 3+ and ATP/Eu 3+ CPNs presented ultrasensitive responses to 2, 6-pyridinedicarboxylic acid, a tunable chromaticity of emission from red to green was achieved by simply varying the doping ratio of Tb 3+ and Eu 3+ into ATP. Moreover, the ATP/Ln 3+ CPNs exhibited surprisingly encapsulation properties for guest dye, indicating the feature of self-adaptive assembly. These features above provided the possibility of performing logic operations. By employing these CPNs as logic operators, the implementation of Boolean logic gates (INH and AND) and integrative logic operation (INH-INH) connecting the elementary logic operations has been successfully achieved.
✓ 已验证
产品关联
DOI
高影响因子
Clinical and Translational Medicine
·
2026
·Vol. 16
·期 2
·e70619
·
IF 7.9
Yifan Ren,Yuxuan Lu,Qing Cui,Hao Shang,Meng Fan,Yun Sun,Xiali Shi,Rongqian Wu,Hongwei Lu
During AP, MFG‐E8 expression in pancreatic acinar cells is downregulated, resulting in disruption of the ABCE1/MFG‐E8 complex. This impairment leads to defective mitophagy flux, accumulation of lipid peroxides, endoplasmic reticulum stress and ferroptosis, ultimately contributing to pancreatic damage .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Tian Yuan,Pu Yang,Shiyu Lv,Kerong Fu,Qiuxian Zhang,Xia Yao,Yanli Luo,Feng Wang
A novel Mg-based defect-modified composite material (MOF-Mg(OH) 2 -HP) was prepared using MOF as a precursor, featuring a unique layered structure with abundant oxygen defects and delocalized electrons on its surface. • MOF-Mg(OH) 2 -HP can be activated by natural light, generating a large amount of reactive species through the “light-Fenton-like” pathway. Superoxide radicals (·O 2 − ) and holes (h + ) are the primary reactive species, which simultaneously oxidize non-reactive organophosphorus compounds and recover orthophosphate (achieving a 100% phosphorus removal rate within 90 min). • It demonstrates exceptional multifunctionality in actual aquaculture wastewater, effectively eliminating 92.84% of Escherichia coli and 98.69% of Chlorella pyrenoidosa , providing a green synergistic solution for integrated water purification and resource recovery strategies. The low-cost and harmless treatment of dairy wastewater is a focal point in agricultural development today. This study innovatively utilizes hydrogen peroxide to modify Mg-MOF-derived oxides, preparing a series of hydrogen peroxide-modified composite materials rich in defects. For the first time, an integrally formed composite material consisting of Mg(OH) 2 with O 2 2− on the outside and MgO on the inside is synthesized. Compared to modified materials prepared using commercially available MgO as the raw material, this material possesses unique Mg(OH) 2 /MgO heterojunctions, a larger specific surface area, abundant oxygen defects, and higher active oxygen content. Starting from the current issues of poor universality, high energy consumption, and difficulties in simultaneous harmless treatment of wastewater in organic phosphorus oxidation materials, we designed an efficient degradation of various organic phosphorus compounds and resource recovery of orthophosphorus using sunlight. This is mainly achieved through the synergistic effect of surface O 2 2− hydrolysis (Fenton-like pathway) and the generation of oxidizing active species through photoexcitation at oxygen vacancies (photocatalytic pathway), resulting in efficient degradation of organic phosphorus and simultaneous recovery (with a removal rate of 100% in 90 min).This material is the first to demonstrate the ability to simultaneously kill 92.84% of Escherichia coli and 98.69% of Chlorella pyrenoidosa during phosphorus removal, proposing a novel collaborative solution for green recovery and harmless treatment of pollutants. Download: Download high-res image (163KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Decai Zeng,Kai Zhang,Haoran Huang,Xiaohua Zhang,Zongde Wang,Shangxing Chen,Guangbin Liu,Yangping Wen,Peng Wang
A general strategy for the in-situ hydrothermal synthesis of transition metal sulfides functionalized hierarchically porous carbons (FeS 2 -HPC, Co 9 S 8 -HPC, Ni 3 S 2 -HPC, Cu 2 S-HPC, and ZnS-HPC) is proposed using different transition metal chlorides as metal sources and activators, sustainable agroforestry waste biomass ( Citrus sinensis peel sponge layer) as a carbon source, sulfuric acid as a sulfur source, and silica colloid as a hard template. The introduction of both transition metal salts and silica colloids facilitate the adjustment of the pore structure. The added sulfuric acid promotes the degradation of the agroforestry waste biomass, which are conducive to the formation of porous carbons. The in-situ synthesis of transition metal sulfide particles and the sulfur doping improve the conductivity, electrocatalytic and enzyme-like activities of obtained composite materials. The FeS 2 -HPC with multiple enzyme-like activities and the best electrocatalytic activity is applied as a nanozyme electrochemical sensing platform for the simultaneous detection of three ATP metabolites with a limit of detection (LOD) of 0.069 μM for uric acid in a linear range of 0.3–200 μM, a LOD of 0.047 μM for xanthine in 0.3–100 μM, and a LOD of 0.21 μM for hypoxanthine in 0.7–300 μM. This work provides a new idea for the development of transition metal sulfides functionalized porous carbons from agroforestry waste biomass, and a theoretical basis for the fabrication of nanozyme electrochemical sensing platform for health assessment and disease prevention.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）