Aquaculture Reports
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2025
·
IF 3.7
Guoyong Huang,Weibin Huang,Guangcai Fu,Yansheng Liu,Hengyang Song,Wenshan Cai,Yubin Liu,Yuxuan Han,Yilin Yao,Beiping Tan,Xiaohui Dong
This study assessed the efficacy of dietary vitamin E in ameliorating growth and liver function in Jinhu grouper compromised by the inclusion of oxidized fish oil (OFO; peroxide value: 121.68 mmol/kg) in the diet, compared with a fresh fish oil control (FFO; peroxide value: 1.25 mmol/kg). The eight-week feeding trial utilized five experimental diets that were isonitrogenous and isolipidic: FFO, OFO (0 mg/kg VE), or OFO supplemented with 200 (VE1), 400 (VE2), or 600 (VE3) mg/kg vitamin E. Fish receiving oxidized fish oil showed slower growth, indicated by reduced weight gain rate (WGR) and increased feed conversion ratio (FCR). Intake of oxidized lipids altered lipid metabolism, lowering serum total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) but elevating low-density lipoprotein cholesterol (LDL-C) and malondialdehyde (MDA) levels. Hepatic antioxidant capacity was compromised, as revealed by the concurrent suppression in both the activities and gene expression levels of key antioxidant enzymes (SOD, CAT, and GPx). Concurrently, oxidized fish oil induced liver inflammation, significantly upregulating pro-inflammatory cytokine genes (notably il-1β , il-6 , il-8 , tnf-α ), and caused histopathological damage including hepatocyte nuclear displacement and lipid droplet accumulation. Vitamin E supplementation ameliorated these effects. The VE2 group (400 mg/kg) maximally restored growth performanc, reduced serum MDA and LDL-C levels, enhanced hepatic antioxidant enzyme activities and gene expression, suppressed inflammatory cytokine expression, and attenuated histopathological lesions. In conclusion, dietary vitamin E supplementation at 400 mg/kg effectively counteracts growth impairment and hepatotoxicity induced by oxidized fish oil in Jinhu grouper.
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高影响因子
FOOD RESEARCH INTERNATIONAL
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2025
·
IF 8
Xuan Zhou,Yingxin Wei,Ziqing Meng,Yiming Feng,Zhaojie Li,Hongwei Zheng,Changhu Xue
This study aims to develop a safe, efficient, and serum-free induction platform for the adipogenic differentiation of adipose-derived stem cells (ASCs) from Larimichthys crocea , to advance the production of cultivated fat for cellular agriculture. Through systematic screening of plant-derived small molecules, bavachinin and dehydroabietic acid were identified as core inducing components, with their concentrations and exogenous lipid supplementation strategies optimised. A serum substitute culture system (SS) was successfully established, comprising growth factors, antioxidants, and carrier proteins, which effectively supports ASCs adipogenesis. Further investigation under three-dimensional culture on gelatin-based porous microcarriers revealed that lower stiffness (10 kPa) enhanced lipid accumulation and adipogenic gene expression. Transcriptomic and metabolomic analyses indicated that the induction system activates the PPARγ pathway and promotes lipid synthesis and accumulation. This work provides a robust chemical biology strategy and theoretical foundation for the scalable production of cultivated fish adipose tissue.
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PubMed
高影响因子
ANALYTICAL SCIENCES
·
2023
·Vol. 39
·期 7
·1129-1142
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IF 1.6
Jin Yating,Hu Di,Shi Cui,Chen Qianqian,Lu Yanbin,Chen Juanjuan
Abstract In the present work, a potential solid-phase extraction (SPE) material based on graphene anchored with platinum nanoparticles (Pt-Graphene) was prepared and characterized by scanning electron micrographs and transmission electron micrograph. The carbamates residues in fish were enriched by SPE filled with Pt-Graphene and detected by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). The proposed extraction protocol exhibited satisfactory recoveries (76.5–115.6%), low limit of quantitation values in μg kg −1 level, and good precision for the studied ten carbamates. These results demonstrated the feasibility of the proposed protocol. The developed Pt-Graphene nanoparticles showed excellent performance for extracting analytes at trace levels, indicating that it could be used as a potential SPE sorbent in food residue analysis. Graphical abstract
研究领域:
分析化学
·
食品安全
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PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 23
·7140
·
IF 4.412
Jingwen Chen,Yinxuan Li,Wenzheng Shi,Hui Zheng,Li Wang,Li Li
This study aimed to develop an active biodegradable bilayer film and to investigate the release behaviors of active compounds into different food matrices. Cinnamaldehyde (CI) or thymol (Ty) was encapsulated inβ-cyclodextrin (β-CD) to prepare the activeβ-CD inclusion complex (β-CD-CI/β-CD-Ty). The tilapia fish gelatin-sodium alginate composite (FGSA) containingβ-CD-CI orβ-CD-Ty was coated on the surface of PLA film to obtain the active bilayer film. Different food simulants including liquid food simulants (water, 3% acetic acid, 10% ethanol, and 95% ethanol), solid dry food simulant (modified polyphenylene oxide (Tenax TA)), and the real food (Japanese sea bass) were selected to investigate the release behaviors of bilayer films into different food matrixes. The results showed that the preparedβ-CD inclusion complexes distributed evenly in the cross-linking structure of FGSA and improved the thickness and water contact angle of the bilayer films. Active compounds possessed the lowest release rates in Tenax TA, compared to the release to liquid simulants and sea bass. CI and Ty sustained the release to the sea bass matrix with a similar behavior to the release to 95% ethanol. The bilayer film containingβ-CD-Ty exhibited stronger active antibacterial and antioxidant activities, probably due to the higher release efficiency of Ty in test mediums.Keywords:biodegradable bilayer film;tilapia fish gelatin;β-cyclodextrin inclusion complex;release kinetics;food simulants;active activities
研究领域:
食品科学
·
材料科学
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高影响因子
Food Bioscience
·
2026
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IF 6.2
Gang Han,Yuhao Zhang,Xu Zhao,Chun Cui
Collagen-rich aquatic materials are promising sources of food-derived bioactive peptides, yet the DPP-IV inhibitory potential of fish maw collagen remains insufficiently explored. In this study, fish maw collagen was hydrolyzed to prepare DPP-IV inhibitory peptides, and their peptide basis and potential inhibitory mechanisms were investigated. Among five commercial proteases, Novozymes 37071 was selected for its superior hydrolytic efficiency and DPP-IV inhibitory activity. A practical hydrolysis condition of 55 °C, 6 h, 2.0% enzyme dosage, and pH 9.0 was selected and verified, yielding a hydrolysate with 97.03% protein recovery, 20.68% degree of hydrolysis, and 66.62% DPP-IV inhibition. The optimized hydrolysate showed good pH and thermal stability, retained inhibitory activity during simulated gastrointestinal digestion, and exhibited concentration-dependent antioxidant activities. LC-MS/MS identified 927 peptides, mainly low-molecular-weight short peptides enriched in proline and hydrophobic residues. A stepwise in silico screening strategy based on abundance, predicted bioactivity, safety, solubility, and ADMET properties narrowed the peptide pool to 22 candidates. Molecular docking suggested that several peptides could interact with key DPP-IV recognition residues through hydrogen bonding and hydrophobic interactions. Among the synthesized peptides, GPA showed the strongest DPP-IV inhibitory activity, with an IC 50 value of 0.464 mg/mL, whereas PGAGF was the most active peptide selected through the virtual screening workflow, with an IC 50 value of 10.449 mg/mL. Kinetic analysis indicated reversible competitive inhibition, and molecular dynamics simulations supported stable PGAGF-DPP-IV and GPA-DPP-IV binding. Overall, fish maw collagen is a promising source of DPP-IV inhibitory peptides for glycemic regulation-oriented food applications.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2016
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IF 3.926
Lu Huang,Haiyun Zhai,Guohuan Liang,Zihao Su,Kaisong Yuan,Guoqiang Lu,Yufang Pan
An analytical technique for selective, simultaneous determination of bisphenol A (BPA) and nonyl phenol (NP) in fish samples was established by a solid-phase extraction chip integrated with array columns of dual-molecularly imprinted polymer-coated silver-modified graphene oxide (Ag/GO-dual-MISPE-chip). Ag/GO dual-molecularly imprinted polymers (Ag/GO-dual-MIPs) were synthesized by in situ polymerization using Ag/GO as the supporting matrix which extracted templates easily. The affinity and specificity on the Ag/GO-dual-MISPE monolithic column were also investigated. The array extraction chip showed high binding capacity and selectivity to BPA and NP, with the imprinting factors of BPA and NP reaching 2.6 and 2.9, respectively. Ag/GO-dual-MIPs were characterized by scanning electron microscopy and Fourier transform-infrared (FT-IR) spectroscopy. As Low as 2.4 ng L −1 BPA and 4.7 ng L −1 NP were detected by high performance liquid chromatography coupled with fluorescence detection. The enrichment factor was 113-fold for BPA and 92-fold for NP. Therefore, the chip-based array columns are feasibly applicable to facile extraction of BPA and NP and effective clean-up of impurities in fish samples.
研究领域:
分析化学
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环境监测
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食品安全
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材料科学
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Hongping Liao,Dandan Gao,Chunmiao Kong,Muhammad Junaid,Ye Li,Xikun Chen,Qingzhi Zheng,Guanglong Chen,Jun Wang
Nanoplastics and di(2-ethylhexyl) phthalate (DEHP) are ubiquitous emerging contaminants that are transferred among organisms through food chain in the ecosystem. This study evaluated the trophic transfer of polystyrene nanoplastics (PSNPs) and DEHP in a food chain including Chlorella pyrenoidosa , Daphnia magna and Micropterus salmoides (algae-crustacean-fish) and lipid metabolism at a higher trophic level in fish. Our results showed that the PSNPs and DEHP accumulated in C. pyrenoidosa or D. magna were transferred to the M. salmoides , of which the DEHP were not biomagnified, while the PSNPs were trophically amplified by the food chain. It is suggested that more PSNPs might be accumulated by higher level consumers in a longer food chain. Additionally, the trophic transfer of PSNPs and DEHP resulted in antioxidant response and histopathological damage in M. salmoides . Moreover, the lipid biochemical parameters and lipid metabolism related genes ( fasn , hsl , cpt1a , atgl , apob , fabp1 , lpl , cetp ) of M. salmoides were significantly affected, which indicated disturbance of lipid metabolism. This study offers great insight into the transfer of contaminants by trophic transfer and their negative effects on organisms at higher trophic levels, which cause human exposure to MNPs and organic contaminants in the ecosystem.
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PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Hongyue Li,Yang Wang,Jiaxin Zhang,Xuepeng Li,Jinxiang Wang,Shumin Yi,Wenhui Zhu,Yongxia Xu,Jianrong Li
Electronic nose (E-nose), electronic tongue (E-tongue) and colorimeter combined with data fusion strategy and different machine learning algorithms (artificial neural network, ANN; extreme gradient boosting, XGBoost; random forest regression, RFR; support vector regression, SVR) were applied to quantitatively assess and predict the freshness of horse mackerel ( Trachurus japonicus ) during the 90-day frozen storage. The results showed that the fusion data of the E-nose, E-tongue and colorimeter could contain more information (with a total variance contribution rate of 94.734 %) than that of the independent one. ANN, RFR and XGBoost showed good performance in predicting biochemical indexes with the R P 2 (the square correlation coefficient of the Test set) ≥ 0.929, 0.936, 0.888, respectively, while SVR models showed a bad performance ( R P 2 ≤ 0.835). In addition, among the established quantitative models, the RFR model had the best prediction effect on K value (freshness index) with R p 2 of 0.936, ANN model had the highest fitting degree in predicting carbonyl content (protein oxidation degree) with R p 2 of 0.978, XGBoost model had the best performance in predicting the TBA value (lipid oxidation degree) with R p 2 of 0.994, RFR model was the best strategy for predicting Ca 2+ -ATPase activity (protein denaturation degree) with R p 2 of 0.969. The results demonstrated that the freshness of frozen fish can be effectively evaluated and predicted by the combination of electronic sensor fusion signals.
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高影响因子
AQUACULTURE NUTRITION
·
2023
·
IF 3.5
Shanshan Wu,Jiamin Li,Yao Deng,Peng Fang,Wei Lei,Ao Luo,Zhengwei He,Liufeng Xiong,Gang Yang,Vikas Kumar,Mo Peng
The aim of this trial was to investigate the effects of apple polyphenols (AP) and taurine (TA) on the growth performance, tissue morphology, and lipid and glucose metabolism in rice field eel fed diets with high oxidized fish oil (OFO). A 10-week feeding experiment was conducted using juveniles (initial body weight 16.66 ± 0.02 g) fed five different diets. Three diets were formulated with various levels of OFO at 9.5, 600, and 800 meq·kg−1 and named as Control, POV600, and POV800 diet, respectively. The other two diets were POV600 and POV800 supplemented with 0.5% AP and 0.2% TA, respectively. Compared to the Control group, only the eels fed POV800 exhibited an increase in weight gain and specific growth rate along with a reduction in feed conversion ratio. AP and TA did not affect growth performance; juveniles fed AP, however, showed a decrease in liver weight. Both POV600 and POV800 decreased nuclei number and increased vacuoles size in the liver. POV800 damaged the intestinal structure integrity and reduced goblet cells number. AP repaired the liver damage on nuclei number and vacuoles size in fish fed with POV600 diet, while TA mitigated intestinal histopathological damage on intact structure and goblet cells number. The mRNA expression level of liver ampkα in fish fed AP was upregulated, while dietary TA upregulated the mRNA expression levels of liver ampkα and accα. In the muscle, POV600 downregulated mRNA expression levels of accα, cpt1, and lipin, whereas POV800 upregulated mRNA expression levels of accα, pparα, and lipin. Dietary AP and TA could counteract the effects of POV600 and POV800 diet on muscle lipid metabolism. Both POV600 and POV800 diets upregulated mRNA expression levels of liver pck1 and gsk3α. AP and TA both downregulated mRNA expression level of liver pck1, while only TA downregulated the expression of liver gsk3α. AP increased the mRNA expression level of gsk3α in muscle. In summary, inclusion of AP and TA did not affect growth performance but showed a potential to alleviate liver or intestinal damages induced by a high OFO diet. Dietary AP and TA were also found to regulate mRNA expression of genes related to lipid and glucose metabolism.
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PubMed
高影响因子
COMPOSITES COMMUNICATIONS
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2021
·
IF 6.617
Aying Zhou,Yingying Zhang,Qingli Qu,Fanghua Li,Tao Lu,Kunming Liu,Chaobo Huang
In recent years, the development of effective antibacterial surfaces and preventing ambient contamination are critically important for medical devices and public hygiene products. In this manuscript, we prepared a multifunctional nanofiber membrane ( PU–CNC–TiO 2 -CNFs-PDMS, PCTCP ) by integrating electrospinning technology and co-blending technique. It is shown that the superhydrophobic composite fiber membrane ( PCTCP ) was endowed with excellent mechanical properties , high elasticity, self-cleaning, anti-contamination, high leakage-proof ability and low cytotoxicity. Furthermore, photothermal and photodynamic behaviors made the fiber film have better bactericidal performance, which will provide additional benefit for medical applications. Hemolysis test demonstrated the ultra-low toxicity of composite fibrous membranes . Finally, the ultrahigh compatibility of fibrous membranes with L929 cells was confirmed by cell imaging using fluorescent confocal microscopy , and the co-culture of PCTCP with zebrafish further demonstrated the potential of fibrous membranes as biomedical materials .
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高影响因子
AQUACULTURE NUTRITION
·
2020
·Vol. 26
·期 3
·954-963
·
IF 2.231
Evelina Emmanuel Wabike,Xun Wu,Wenliang Zhu,Bao Lou,Ruiyi Chen,Dongdong Xu,Ligai Wang,Shanshan Zhou,Peng Tan
The imbalance of fish oil (FO) supply and demand has motivated efforts to identify an alternative for aqua feed. An 8-week feeding trial was conducted to evaluate the effects of dietary fish oil partial replacement with mixed oil from linseed and lard (1:1) on growth, body composition and immunity when the N3 long-chain polyunsaturated fatty acid (N3 LC-PUFAs) requirement is met for Nibea albiflora . Two types of experimental diets were formulated with 100% fish oil (FO) or 69% mixed oil (Mix). The results indicated that the Mix diet significantly improved the specific growth rate (SGR), weight gain (WG), feed efficiency ratio (FER), muscle tissue growth hormone receptor ( GHR ), insulin-like growth factor 1 ( IGF1 ) and insulin-like growth factor 1 receptor ( IGF1R ) gene expression of the yellow drum ( p < .05). No significant difference in the survival rate (SR), feed intake (FI), hepatosomatic index (HSI), viscerosomatic index (VSI), condition factor (CF), N3 PUFA percentage, liver antioxidant enzyme activities or pro-inflammatory gene expression was observed between the two treatments. Therefore, terrestrial blend lipid from linseed and lard could be used as an alternative for dietary fish oil without compromising growth performance and immunity for the juvenile yellow drum.
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高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 8
·1871-1885
·
IF 2.413
Xu Lijun,Miao Xiaohuan,Yang Zhaoguang,Li Haipu,Qiu Bo
Solid-phase extraction (SPE) was combined with dispersive liquid-liquid microextraction based on solidification of floating organic droplet (DLLME-SFO) as a pretreatment technique for the simultaneous analysis of 11 organochlorine pesticides (OCPs) and 5 polychlorinated biphenyls (PCBs) in fish tissue. Gas chromatography with electron capture detector was used for the identification and quantification of these compounds. In the newly developed method, the acetonitrile extractant of the fish sample was cleaned up by commercialized SPE cartridges packed with acid alumina, and then mixed with water, sodium-chloride, and n-pentadecane for the purpose of target analyte enrichment via DLLME-SFO process. Effect of the parameters including SPE packing material, amount and chemical nature of extraction solvent, water volume, and salt concentration on the signal responses were investigated, and the recoveries of this method ranged between 70.1% and 112.3% at three spiked levels under the optimized conditions. The calibration curves were found to be linear in the range of 1.56–312.5 μg L −1 , and the limits of detection ranged from 0.372 to 1.12 μg kg −1 . Comparing the other reported pretreatment method, the presented one was sensitive, time-saving, and environmentally friendly.
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高影响因子
危险品化学品经营许可证（带存储）