SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Ni Hui,Shiying Wang,Hongbo Xie,Shenghao Xu,Shuyan Niu,Xiliang Luo
A facile two-step electrochemical strategy was reported to synthesize nanocomposite of reduced graphene oxide (RGO) doped conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) decorated with nickel nanoparticles (NiNPs) onto a glassy carbon electrode (GCE). Pure graphene oxide (GO) doped PEDOT composite was firstly electropolymerized onto the GCE through cyclic voltammetry, followed by electrochemical reduction in a solution containing nickel cations at a constant potential of −0.9 V. During the electrochemical reduction process, GO doped in the PEDOT composite would be reduced to a more conductive form of RGO, and at the same time, nickel cations could be reduced to form NiNPs and loaded on the composite surface. The prepared nanocomposite (NiNPs/PEDOT/RGO) modified electrode showed outstanding electrocatalytic activity toward the oxidation of glucose in alkaline media, and it could be developed into a nonenzymatic glucose sensor. Under optimum conditions, the glucose sensor exhibited a linear range from 1.0 μM to 5.1 mM and a detection limit of 0.8 μM (S/N = 3), associated with excellent stability, high reproducibility and favorable selectivity against common interferents. Furthermore, the nonenzymatic sensor was also successfully applied to the detection of glucose in human serum samples, showing promising potential in the clinical application.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2015
·
IF 3.545
Huifang Hu,Miao Feng,Hongbing Zhan
An amperometric glucose biosensor based on direct electron transfer of glucose oxidase (GOD) self-assembled on the surface of partially unzipped carbon nanotubes (PUCNTs) modified glassy carbon electrode (GCE) has been successfully fabricated. PUCNTs were synthesized via a facile chemical oxidative etching CNTs and used as a novel immobilization matrix for GOD. The cyclic voltammetric result of the PUCNT/GOD/GCE showed a pair of well-defined and quasi-reversible redox peaks with a formal potential of −0.470 V and a peak to peak separation of 37 mV, revealing that the fast direct electron transfer between GOD and the electrode has been achieved. It is notable that the glucose determination has been achieved in mediator-free condition. The developed biosensor displayed satisfactory analytical performance toward glucose including high sensitivity (19.50 μA mM −1 cm −2 ), low apparent Michaelis–Menten (5.09 mM), a wide linear range of 0–17 mM, and also preventing the interference from ascorbic acid , uric acid and dopamine usually coexisting with glucose in human blood. In addition, the biosensor acquired excellent storage stabilities. This facile, fast, environment-friendly and economical preparation strategy of PUCNT–GOD may provide a new platform for the fabrication of biocompatible glucose biosensors and other types of biosensors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2016
·
IF 4.803
Huifang Hu,Yingpan Song,Miao Feng,Hongbing Zhan
The one dimensional (1D) carbon nanomaterials have been conceived as the suitable candidate for the biosensor, but the effect of their structures on the biosensing properties was deficient. Here, we chose carbon nanotubes (CNTs), graphene nanoribbons (GONRs) as models for 1D and quasi 1D carbon nanomaterials, and partially unzipped CNTs (PUCNTs) as an intermediate material, to investigate structure-dependent electrocatalysis of 1D carbon nanomaterials. The 1D to quasi 1D carbon nanomaterials with various surface areas, defect densities and oxygen contents were used to modify the electrodes and exhibited excellent electrocatalytic activities and performances towards UA and DA without the influence of AA . The CNTs with low oxygen and defect content helped to induce direct electron transfer and displayed a highest sensitivity; with abundant oxygen functional groups, the GONRs exhibited a broadest linear detection range; for the intermediate material, PUCNTs showed secondary electrochemical response for UA and DA. Additionally, the 1D carbon materials were applied in the real samples with satisfactory results. The 1D carbon materials demonstrated different electrochemical behaviors and thus can provide rational choices for developing higher sensitivity and wider linear range biosensors by optimizing and controlling the nanostructure.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Zhiming Jiang,Guangyu Li,Mingxiao Zhang
A novel electrochemical sensor for gatifloxacin based on one-pot electro-polymerization of β-cyclodextrin (β-CD) and reduced-graphene oxide (rGO) modified glassy carbon electrode (GCE) (β-CD/rGO/GCE) was built for the first time. Scanning electron microscope (SEM) image and Infrared spectroscopy (IR) show that polymer of β-cyclodextrin and reduced graphene oxide (rGO) has been successfully modified on electrode. The electrochemical properties of this polymer were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The optimization of the electro-polymerized cycle, the pH of supporting electrolyte and the accumulation potential and time were discussed in details. Under optimum conditions, the differential pulse voltammogram (DPV) exhibited the oxidation peak currents were linearly proportional to gatifloxacin concentrations in the range from 0.05 μM to 150 μM with a sensitivity of 0.33 μA μM −1 . This electrochemical sensor had a low detection limit of 0.02 μM ( S/N = 3). Moreover, this proposed sensor exhibited good reproducibility, long-term stability and fast current response. To further study the practical applicability of the proposed sensor, the modified electrode was successfully applied to detect gatifloxacin in tablets and human urine samples.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2016
·
IF 4.035
Yating Xu,Ju Liu,Hongyang Zhang,Min Jiang,Lingling Cao,Min Zhang,Wei Sun,Shengli Ruan,Ping Hu
In this study, hypercrosslinked strong cation-exchange polymer resins (HXLPP-SCX) were synthesized and employed as selective sorbents for the solid-phase extraction (SPE) of basic purine metabolites associated with gout. The HXLPP-SCX material was prepared based on hypercrosslinking reactions and sulfonated with concentrated H 2 SO 4 . This synthetic procedure is facile and efficient without using highly toxic reagent. The resulting resins were characterized in the form of monodisperse microspheres (mean diameters of 3‒5 μm) with narrow pore size (2.1 nm) and relatively high specific surface areas (801 m 2 /g). The polymers also possess high ion-exchange capacity (IEC, 2.22 mmol/g) and good adsorption and selectivity performances for basic compounds. The resins used as SPE sorbents permit the selective enrichment of three pivotal purine metabolites (hypoxanthine, xanthine and inosine) in human serum followed by HPLC analysis. Method validation including linearity range, sensitivity, accuracy and reproducibility were evaluated. This method was exemplarily applied in the analysis of serum purines in gout patients and healthy controls. The present results demonstrate a promising potential of this HXLPP-SCX material for the clinical sample pretreatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Jian Ju,Ruizhong Zhang,Wei Chen
In this work, a novel colorimetric sensor is designed for the sensitive detection of glutathione (GSH) based on the intrinsic peroxidase-like activity of Ag nanoparticles supported on the nitrogen-doped graphene quantum dots (Ag NPs-N-GQDs). The Ag NPs-N-GQDs nanocomposites are prepared by a facile one-step photocatalytic strategy and only N-GQDs were used as photocatalytic agents. Therefore, the surface of the obtained Ag NPs is clean without the capping of surfactant. TEM, XRD and XPS characterizations clearly show that Ag NPs-N-GQDs have been successfully prepared. Such non-surfactant capped Ag NPs provide naked catalytic surface with highly catalytic activity, which can catalyze the oxidation of 3,3′5,5′-tetramethylbenzidine (TMB) to oxTMB with the presence of H 2 O 2 along with a visual color variation. Since GSH can reduce oxTMB, the UV–vis absorption intensity of oxTMB shows a strong dependence on the concentration of GSH. The novel Ag NPs-N-GQDs-TMB-H 2 O 2 system exhibits high sensitivity and selectivity for the detection of GSH with a low detection limit of 31 nM and a wide detection linear range of 0.1–157.6 μM. Furthermore, the novel sensor system shows great potential application for GSH detection in blood serum sample, and easy-to-make analytical approaches in the future.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2015
·
IF 2.729
Taotao Yang,Jingkun Xu,Limin Lu,Xiaofei Zhu,Yansha Gao,Huakun Xing,Yongfang Yu,Wanchuan Ding,Zhen Liu
Cu nanoparticle/graphene oxide/single walled carbon nanotube (CuNP/GO/SWCNT) composites were prepared by a facile electrodeposition method and used for constructing nonenzymatic glucose sensor. Scanning electron microscopy (SEM) and Raman spectroscopy were employed to characterize the morphology and structures of the samples. The electrocatalytic performance of CuNP/GO/SWCNT composites towards glucose oxidation was studied by cyclic voltammetry (CV) and current–time measurements. Electrochemical results indicated that CuNP/GO/SWCNT electrode exhibited a higher electrocatalytic activity towards the oxidation of glucose than CuNP, CuNP/GO and CuNP/SWCNT electrodes. This was because the GO/SWCNT composite as substrate material not only possessed excellent conductivity, but also provided large surface area for the high loading of the CuNPs. Meanwhile, the good dispersibility, independent and multi-layer structure of CuNP, could enhance the charge-transport properties, and afford more active sites for the catalytic oxidation of glucose. Under the optimized conditions, the sensor showed a high sensitivity of up to 930.07 μA mM − 1 cm − 2 , with a wide linear range of 1 μM to 4.538 mM and a low detection limit of 0.34 μM (S/N = 3). It also exhibited excellent stability, reproducibility, selectivity, and reliable measurement in real human blood samples. All of these excellent properties made the CuNP/GO/SWCNT composite material promising for the development of effective nonenzymatic glucose sensors.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Qu Sheng,He Mei,Huimin Wu,Xiuhua Zhang,Shengfu Wang
Platinum–cobalt Pt x Ni alloy nanoparticles have been successfully fabricated by the chemical reduction method, using carbon (Vulcan XC-72) as support. The resulting modified glassy carbon electrode shows excellent catalytic activity for the oxidation of glucose. Under physiological conditions , the oxidation current of glucose has two specific linear ranges of 2.0 − 420 μM and 0.5–8.5 mM, with high sensitivities of 1795.1 μA mM −1 cm −2 and 707.72 μA mM −1 cm −2 , respectively. In addition, interference from the oxidation of ascorbic acid , uric acid , or acetamidophenol could be effectively avoided. The non-enzymatic glucose sensors based on this electrode exhibited excellent selectivity, stability, and repeatability. Thus, this novel non-enzyme sensor has potential application in glucose detection.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Lihong Li,Zhao Li,Wen Shi,Xiaohua Li,Huimin Ma
A highly sensitive and selective near-infrared (NIR) fluorescent probe, (E)-2-(2-(6-((2-carboxy-8-oxo-7-(2-phenylacetamido)-5-thia-1-azabicyclo[4.2.0]oct-2-en-3-yl)methoxy)-2,3-dihydro-1H-xanthen-4-yl)vinyl)-3,3-dimethyl-1-propyl-3H-indol-1-ium (1), is developed for the determination of β-lactamase. The probe is designed and synthesized by incorporating the specific substrate (cephalosporin) of β-lactamase into a stable hemicyanine skeleton. The fluorescence of 1 itself is very weak due to the alkylation of the hydroxyl group of the hemicyanine fluorophore; however, β-lactamase can selectively react with its substrate (β-lactam ring) in the probe, thereby causing a spontaneous fragmentation. This action leads to the release of the fluorophore and a large fluorescence enhancement at 707 nm (λex = 680 nm). Under the optimized conditions, the fluorescence response of probe 1 is directly proportional to the concentration of β-lactamase in the range of 0.05–2 nM, with a detection limit of 0.02 nM. The validity of the probe has been confirmed by determining β-lactamase in human urine samples in comparison with that determined by iodimetry. Moreover, by taking advantage of its high sensitivity and NIR emission feature, the probe has also been utilized to image β-lactamase in three types of Staphylococcus aureus, including methicillin-resistant S. aureus ATCC BAA44, penicillin-resistant strain ATCC 11632, and penicillin-susceptible strain ATCC 29213, which clearly reveals the significantly different expression levels of β-lactamase in these S. aureus.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Lin Jiang,Yaping Ding,Feng Jiang,Li Li,Fan Mo
A nitrogen-doped graphene/carbon nanotubes (NGR–NCNTs) nanocomposite was employed into the study of the electrochemical sensor via electrodeposition for the first time. The morphology and structure of NGR–NCNTs nanocomposite were investigated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. Meanwhile, the electrochemical performance of the glassy carbon electrode (GCE) modified with electrodeposited NGR–NCNTs (ENGR–NCNTs/GCE) towards caffeine (CAF) and vanillin (VAN) determination was demonstrated by cyclic voltammetry (CV) and square wave voltammetry (SWV). Under optimal condition, ENGR–NCNTs/GCE exhibited a wide linearity of 0.06–50 μM for CAF and 0.01–10 μM for VAN with detection limits of 0.02 μM and 3.3 × 10 −3 μM, respectively. Furthermore, the application of the proposed sensor in food products was proven to be practical and reliable. The desirable results show that the ENGR–NCNTs nanocomposite has promising potential in electrocatalytic biosensor application.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Qi Wang,Qian Zhou,Qicheng Zhang,Rongjia Shi,Shangshang Ma,Wenbo Zhao,Min Zhou
Preparation of high performance electrochemical biosensing interface for the sensitive and rapid detection of human metabolites is of great interest for health care and biomedical science. In this paper, based on the adhesion technique of marine mussels, we designed and prepared a novel biosensor with a micro/nano-biointerface of Fe 3 O 4 -Mn 3 (PO 3 ) 2 @Ni foam, which offered a three dimensional (3D) living environment for real cell. The constructed biosensor with a 3D micro/nano-biointerface of Fe 3 O 4 -Mn 3 (PO 3 ) 2 @Ni foam was characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and elemental mapping. Furthermore, the electrochemical experiments by electrochemical method for detection of superoxide anion (O 2 •− ) in situ released by cells were carried out by this biosensor we proposed. Results indicated that the 3D interface of mussel-inspired Fe 3 O 4 -Mn 3 (PO 3 ) 2 @Ni foam offered an amicable platform for promoting cell adhesion, which was beneficial for enhancing biosensing activity. This proposed sensing platform provided high electroactivity and excellent electron transport with a lower detection limit (0.0170 μM), wider linear range 0.04–2.44 μM) and short diffusion distance to reaction sites. The case achieved the accurate detection of O 2 •− (in situ released by cells) based on the combination of mussel-inspired biomimetic adhesion technique, 3D micro/nano-biointerface construction and electrochemical biosensing technique.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Wenjun Zhao,Jiayi Jin,Huimin Wu,Shengfu Wang,Chuanqi Fneg,Shuijin Yang,Yu Ding
The Cu@Pt/C nanocomposites have been synthesized via two-step reduction method. Electrochemical observations showed that the Cu@Pt/C had better electrocatalytic activity for the reduction of hydrogen peroxide than Pt/C, with a wide linear range between 0.50 μM and 32.56 mM, a high sensitivity of 351.3 μA mM − 1 cm − 2 , and a low detection limit of 0.15 μM (signal/noise = 3). Furthermore, the sensor based on Cu@Pt/C has potential applications due to its excellent long-time stability, good reproducibility and acceptable selectivity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）