Pharmaceutics
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2025
·Vol. 17
·期 2
·153
·
IF 5.5
Yan Yang,Qu Lin,Zhenhai Hou,Gensheng Yang,Lian Shen
Objectives: Treatment of osteochondral defects is hindered by several challenges, including the failure of traditional scaffolds with a predefined cylindrical or cuboid shape to comprehensively match the natural osteochondral tissue. Herein, we employed reverse modeling and three-dimensional (3D) printing technologies to prepare subchondral bone and cartilage. Methods: The osteochondral scaffold was prepared by bonding the subchondral bone and cartilage layers, and the curvature distribution and biomechanical behavior were compared with those of the native tissue. Biocompatibility and osteochondral regeneration performance were further evaluated using cell adhesion and proliferation assays, as well as animal osteochondral defect repair tests. Results: We found that increasing the printing temperature or decreasing the layer height improved the dimensional accuracy of printed subchondral bones, whereas increasing the exposure time or decreasing the layer height enhanced the dimensional accuracy of the printed cartilage. Biomimetic scaffolds exhibited curvature distribution and biomechanical behavior more similar to native tissues than traditional cylindrical scaffolds. Incorporating gelatin methacryloyl into poly (ethylene glycol) diacrylate markedly improved the biocompatibility, and correspondingly prepared osteochondral scaffolds had better osteochondral regeneration ability than the traditional scaffolds. Conclusions: Osteochondral scaffolds exhibiting biomimetic morphology and an internal structure could be prepared based on reverse modeling and 3D printing, facilitating personalized osteochondral injury treatment.
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Materials Advances
·
2024
·Vol. 5
·期 6
·2456-2469
·
IF 5.2
Haihua Zhou,Hongyan Pan,Faisal Raza,Hajra Zafar,Yu Ge,Nan Wang,Ronglei Zheng,Degeng Zhang,Yanmin Yang
Cancer is a grave threat to human life and well-being today. Photothermal therapy, a non-invasive method that converts light energy into heat energy to eliminate tumor cells, has emerged as a promising approach. In this study, bismuth nanosheets were utilized as photothermal agents, while 5-fluorouracil and metformin served as model drugs. By designing a temperature-sensitive liposome (BiNSs/Met/5-FU@TSL) that encapsulates bismuth nanosheets, a multifunctional drug delivery system was developed, combining photothermal therapy with synergistic chemotherapy for enhanced anti-tumor effects. Through transmission electron microscopy, it was confirmed that bismuthene nanosheets were successfully encapsulated in stable and uniformly spherical liposomes. Photothermal performance tests demonstrated the system's effectiveness and stability in generating heat. In vitro release experiments further confirmed the thermosensitive liposomes’ responsiveness to photothermal stimuli. Both in vitro and in vivo studies showcased the system's excellent anti-tumor effects and the safety of the blank carriers. This research presents a novel concept, providing insights into the combination of tumor photothermal therapy with chemotherapy, opening new avenues for effective cancer treatment.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Min Liu,Lei Liang,Chaojie Yu,Bingyan Guo,Haitao Zhang,Fanglian Yao,Hong Zhang,Junjie Li
Cell cryopreservation is crucial for biomedicine, and the addition of cryoprotectants (CPAs) to protect cells from cryoinjuries is essential at low temperatures. Trehalose (Tre) has been considered a biocompatible CPA because of its unique physicochemical properties, which could provide cryoprotection to cells during cryopreservation by its interaction with cell membrane phospholipids. Nonetheless, there were few studies on the synthesis of Tre-based CPAs. Here, we reported a feasible solution to further improve the cell cryopreservation efficiency by modifying Tre with sulfoxide functional groups, which enabled dimethyl sulfoxide (DMSO)-free cryopreservation of cells. Compared with unmodified Tre, the sulfoxide-modified Tre (Tre-S) could further protect cells from cryoinjuries. At the optimal CPA concentration (8% Tre-S), the survival rate of L929 cells was up to 88.5% after cryopreservation, and the recovered cells could maintain their normal cell biological behaviors. The differential scanning calorimetry (DSC) and ice recrystallization inhibiting (IRI) tests show that Tre-S has good melting point reduction and IRI activities. Compared with DMSO, the Tre-S had good biocompatibility. This study may provide an alternative method for DMSO-free cell cryopreservation.
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MOLECULES
·
2024
·Vol. 29
·期 6
·1292
·
IF 4.2
Siyue Guan,Yue Wang,Ting Hu,Lingling Che,Xiaoqiao Wang,Yike Huang,Zhining Xia
Molecular imprinting technology is widely used for the specific identification of compounds, but the selective recognition mechanisms of the same compounds still need to be further studied. Based on differences in hydrogen bond size and orientation, molecularly imprinted polymers (MIPs) were designed to adsorb flavonols with the same parent core and different hydroxyl groups. A surface-imprinted material was designed with silicon dioxide as the carrier, myricetin as the template molecule, and methacrylic acid (MAA) as the functional monomer. Scanning electron microscopy (SEM), Brunauer–Emmett–Teller surface area (BET) analyses, Fourier-transform infrared spectroscopy (FT-IR), and other characterization experiments were carried out. The intrinsic mechanism of the MIPs was also explored. The MIPs showed good adsorption of myricetin and other flavonoids through hydrogen bonding and steric hindrance. The adsorption capacity was 3.12–9.04 mg/g, and the imprinting factor was 1.78–3.37. Flavonoids with different hydroxyl groups in different numbers and directions had different hydrogen bond strengths with functional monomers. R2, R4, and R1on 2-phenylchromogenone had stronger electronegativity, and the hydroxyl group was also more likely to form and have stronger hydrogen bonds. The hydroxyl negativity and the degree of steric hindrance of flavonoids played a major role in the recognition of molecularly imprinted materials. This study is of great significance for the synthesis of and selection of templates for analogous molecular imprinting materials.
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International Journal of Pharmaceutics-X
·
2024
·
IF 5.2
Liangxing Tu,Ping Han,Yongbing Sun,Yi Jin,Kaili Hu,Meng Cheng,Yisen Shao,Jianfang Feng,Fangying Yuan
Many researchers have studied the oral absorption mechanisms yet, however, considering stabilizers often participate in the absorption process of nanocrystals, these known mechanisms may be incorrect. Hence in this study, we aimed to explore the correct absorption mechanism of nanocrystals by performing related studies on stabilizer-free nanocrystals. We firstly prepared stabilizer-free silymarin nanocrystals by high-pressure homogenization, and then performed absorption-related studies, such as solubility, dissolution rate, pharmacokinetic study, cellular uptake and intracellular transport. Results showed the stabilizer-free silymarin nanocrystals had an average particle size of (450.2 ± 4.46) nm, with PDI of 0.280 ± 0.021 and Zeta potential of −26.9 ± 2.4 mV. The conversion of silymarin crude drug to stabilizer-free silymarin nanocrystals increased the compound's solubility by 1.41 times, with a dissolution rate of 92.2 % in water within 30 min compared to 38.5 % for crude drugs. Pharmacokinetic studies showed the oral bioavailability of stabilizer-free silymarin nanocrystals was found to be 1.48 times greater than that of the crude drugs. The cell experimentation results demonstrated that the stabilizer-silymarin nanocrystals can improve uptake but have poor transmembrane transport properties. Most researchers believe that nanocrystals can enhance transmembrane transport of drugs via an endocytosis-mediated pathway. In fact, nanocrystals are indeed endocytosed more by the cells, but this transport pathway is poor because the cells lack the intracellular transport pathway to transport nanocrystals from the AP side to the BP side. Therefore, we believe that the intracellular transport of nanocrystals can be enhanced by modifications and other carriers if needed to improve nanocrystals' ability to promote oral absorption.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2025
·
IF 8.3
Xu Zhang,Hui Wang,Xiangyu Wang,Ning Wang,Xuehua Wu
The liquid organic hydrogen carriers (LOHCs) system has attracted much attention because of its promising characteristics. In this paper, 4-methylindole (4-MID), was selected and studied in hydrogenation and dehydrogenation processes as a potential liquid organic hydrogen carrier (LOHC) because of its theoretical hydrogen storage density of 5.76 wt%, a melting point of 5 °C and a boiling point of 267 °C. Full hydrogenation of 4-MID was carried out over a 5 wt% Ru/Al 2 O 3 catalyst in the range of 160–200 °C at 7 MPa. The results showed that the hydrogenation reaction rate was positively correlated with the temperature, and the apparent activation energy was 67.3 kJ/mol. The structure of the hydrogenation intermediates was calculated to determine a reasonable reaction pathway by means of Material Studio calculations. The full hydrogenation product, was dehydrogenated over a 5 wt% Pd/Al 2 O 3 catalyst in the range of 150–180 °C at 101 kPa. The fully dehydrogenation was accomplished at 150 °C for 70 min. The cycling performance of 4-MID was investigated and it was found that after three cycles, its hydrogenation and dehydrogenation performance was good.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Lei Xie,Tianxing Wang,Ling Chen,Xiaoxi Li
Our previous research discovered that myricetin could effectively inhibit the formation of heterocyclic aromatic amines (HAAs) in cantonese baked foods by trapping phenylacetaldehyde to form adducts. However, the structure and biological activity of these adducts were still unknown. In this study, we identified two myricetin-phenylacetaldehyde adducts from cantonese mooncakes, BYQ-2 and BYQ-3, using pre-HPLC. These adducts were found to be the products of phenylacetaldehyde addition at the C-8 and C-6 positions of myricetin, followed by cyclization with hydroxyl groups at the C-7 and C-5 positions. Antioxidant assays revealed that BYQ-2 and BYQ-3 have stronger radical scavenging abilities than myricetin in the concentration range of 12.5 ∼ 800 μg/mL in the three samples. Additionally, both adducts showed potential health benefits by inhibiting α-glucosidase and CYP450 1A2, enzymes involved in blood sugar regulation and HAAs metabolism. BYQ-2 exhibited the highest inhibitory potency against α-glucosidase and CYP450 1A2 with IC 50 values of 10.32 μg/mL and 5.44 μg/mL, respectively. Theoretical calculations suggested that hydrogen bonding and hydrophobic interactions are the primary forces driving enzyme binding, with BYQ-2 showing the highest binding energies (−7.75 kcal/mol for α-glucosidase and −9.31 kcal/mol for CYP450 1A2). Our findings suggested that myricetin inhibited HAAs in baked foods while also enhancing food safety and providing health benefits through its adduction with small molecule aldehydes. In future research, it is necessary to further evaluate the absorption and metabolic behavior as well as safety of myricetin-HAAs active intermediate adducts at the cellular and animal experimental level.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2024
·Vol. 110
·期 3
·910-921
·
IF 2.3
Jin Xiaotong,Yuan Kangkang,Ji Xingxiang,Li Chengshun
Porosity and thermal stability are the main constraints that determine the performance of zirconia (ZrO 2 ) for a wide range of applications, including adsorption, catalysis, filtration, etc. Here, we have proposed the rational design of electrospun ZrO 2 nanofibers (NFs) with high surface areas and good thermal stability. Water vapor pre-treatment was used to modify the surface structure of the NFs with the removal of soft templates at a lower temperature. In addition, amorphous B 2 O 3 was introduced into the ZrO 2 NFs to improve the thermal stability of the porous structure. The as-prepared NFs had high surface area of 380 m 2 /g and even 300 m 2 /g after heat treatment at 450 °C. The catalytic activity of modified ZrO 2 NFs as support materials in bromination reaction of phenol red was tested. And a high specific bromination activity of 3.02 mmol h −1 g −1 was obtained. This work could provide promising strategies for preparing electrospun porous oxide NFs with high surface area and good thermal stability in order to optimize performance. Graphical Abstract
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Journal of Energy Chemistry
·
2024
·
IF 14
Ming-Hao Guan,Hao-Nan Xu,Jin Liu,Tao Wu,An-Hui Lu
Regulating the intermediates involved in the electrocatalytic nitrate reduction reaction (NO 3 RR) is crucial for the enhancement of reaction efficiency. However, it remains a great challenge to regulate the reaction intermediates through active site manipulation on the surface of the catalyst. Here, a family of n %-Co 3 O 4 /SiC ( n = 5, 8, 12, 20) catalysts with a delicate percentage of Co 2+ and Co 3+ were prepared for NO 3 RR. We found that Co 3+ primarily acts as the active site for NO 3 − reduction to NO 2 − , while Co 2+ is responsible for the conversion of NO 2 − to NH 3 . Moreover, the conversion of these intermediates over the active sites is autonomous and separately controllable. Both processes synergistically accomplish the reduction of nitrate ions to synthesize ammonia. Combining the experimental studies and density functional theory (DFT) calculations, it is discovered the pathway (*NHO→*NHOH→*NH 2 OH→*NH 2 →*NH 3 ) is more favorable due to the lower Δ G value (0.25 eV) for the rate-limiting step (*NO→*NHO). The NH 3 yield rate of 8%-Co 3 O 4 /SiC reached 1.08 mmol/(cm 2 h) with a Faradaic efficiency of 96.4% at −0.89 V versus the reversible hydrogen electrode (RHE), surpassing those of most reported non-noble NO 3 RR catalysts. This strategy not only provides an efficient catalyst for NO 3 RR but also serves as an illustrative model for the regulation of multi-step reaction intermediates through the design of distinct active sites, thereby presenting a new approach to enhance the efficiency of intricate reactions.
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ChemSusChem
·
2024
·
IF 7.5
Kaidan Yang,Ding Zhou,Yachao Wang,Ruina Chen,Qi Dong,Pu Xiao,Yingshan Zhou,Jing Zhang
Hyaluronic acid hydrogels are promising materials for diverse applications, yet their potential is hampered by limitations such as low self-healing efficiency and insufficient mechanical strength. Inspired by the heterogeneous structures of spider silk, we introduce a novel dual dynamically crosslinked network hydrogel. This hydrogel comprises an acylhydrazone-crosslinked network, utilizing aldehyde hyaluronic acid (AHA) and 3,3'-dithiobis (propionohydrazide) (DTP) as a first network, and a secondary network formed by hydrogen bonds-crosslinked network between tannic acid (TA) and silk fibroin (SF) with β-sheet formation. The hydrogel exhibits exceptional self-healing ability due to the dynamic and reversible nature of Schiff base bonds, disulfide bonds, and hydrogen bonds, achieving complete healing within 5 minutes. Additionally, the spider silk-inspired heterogeneous structures enhance mechanical properties. Furthermore, the incorporation of TA provides enhanced adhesion, as well as remarkable antibacterial and antioxidant properties. This innovative hyaluronic acid-based hydrogel, inspired by spider silk, offers a promising avenue to fortify both the mechanical strength and self-healing capabilities of hydrogels, thus expanding opportunities for applications in tissue engineering and biomedicine.
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MACROMOLECULES
·
2024
·
IF 5.1
Kai Geng,Tengyao Xue,Xiaodan Li,Haoqi Sang,Shichao Feng,Chenyi Wang,Hanli Wang,Yongbing Zhuang
In this study, a novel diamine monomer (BTPA) containing a benzocyclobutene (BCB) cross-linking site was synthesized, and two polyimides (PIs), PI-BCB-1 and PI-BCB-2, were synthesized using BTPA with dianhydrides, 4,4′-(hexafluoroisopropyl) diphenyl anhydride (6FDA) and 4,4′-(4,4-isopropylidenediphenoxy)bis(phthalic anhydride) (BPADA), respectively. The BCB-based PIs were soluble in common organic solvents and exhibited a desirable toughness. The cross-linking behavior of the BCB-based PIs was investigated using nonisothermal differential scanning calorimetry, and a mathematical model for the curing kinetics was established. Further, the effects of BCB cross-linking on the interchain packing, thermal performance, and gas transport properties of the PIs were thoroughly examined. The cross-linked PIs exhibited excellent heat resistance with glass-transition temperatures exceeding 400 °C. In addition, the CO2 and CH4 permeabilities for the cross-linked PI membranes increased 16- and 27-fold, respectively, compared with their counterparts before cross-linking. Remarkably, the CO2 permeability for PI-BCB-1 increased from 33 to 1559 Barrer after cross-linking, while maintaining a nearly unchanged CO2/CH4 selectivity of 35 (39 for its uncross-linked counterpart), surpassing the 2008 Robeson upper limit. In addition, the cross-linked PI membranes showed desirable aging resistance and excellent plasticization resistance. This study offers insights for designing PIs containing BCB side group-cross-linked units to enhance membrane-based gas separation performance.
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JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Renjie Xu,Ganbing Yao
Equilibrium solubility of fenoxaprop-p-ethyl in aqueous mixtures of ethanol/ isopropanol/acetonitrile/ N,N -dimethylformamide (DMF) plus water was experimentally measured by isothermal saturation method at (278.15–323.15) K under 101.2 kPa. The order of solubility (mole fraction) of fenoxaprop-p-ethyl in pure solvents from high to low is 4.233 × 10 −2 (DMF 323.15 K) > 2.044 × 10 −2 (acetonitrile 323.15 K) > 7.727 × 10 −3 (isopropanol 323.15 K) > 5.338 × 10 −3 (ethanol 323.15 K) > 2.565 × 10 −5 (water 323.15 K). Experimental results showed that temperature and type of solvent were decisive factors for the dissolution of fenoxaprop-p-ethyl. The content of fenoxaprop-p-ethyl in solution was analyzed by high-performance liquid chromatography (HPLC). Moreover, the solubility data of fenoxaprop-p-ethyl was correlated by Jouyban-Acree (J-A) model, van’t Hoff-Jouyban-Acree (V-J-A) model and Apelblat-Jouyban-Acree (A-J-A) model. The maximum of root-mean-square deviation ( RMSD ), relative average deviation ( RAD ) and standard deviation ( SD ) predicted via three thermodynamic models were 1.78 × 10 −4 (DMF + water), 2.87 × 10 −2 (acetonitrile + water) and 1.79 × 10 −4 (DMF + water), respectively. In addition, the solvent effect between solute and solvent molecules was deduced by KAT-LSER model and the result showed that dipolarity/polarizability interactions between the solute and solvent molecules were dominant in the dissolution of fenoxaprop-p-ethyl. Finally, the preferential solvation parameter ( δx 1,3 ) of fenoxaprop-p-ethyl was obtained by the method of inverse Kirkwood–Buff integrals and the preferential dissolution behavior of each concentration range is explained by Lewis acid base theory. However, for acetonitrile + water, the change of δx 1,3 may be due to the polarization effects and it is not suitable for Lewis acid base theory.
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