INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Chang-Wei Zhang,Ming-Fei Li,Ran Tao,Mi-Jun Peng,Zhi-Hong Wang,Zhi-Wen Qi,Xing-Ying Xue,Cheng-Zhang Wang
Polyprenol is an important active ingredient in Ginkgo biloba leaves (GBL) with great industrial application potential. In this paper, a novel high energy ball milling extraction technology coupled with silver-thiolate material purification method was developed, with which a high purity of GBL polyprenol (GBP) was obtained. Results showed that this method had a high extraction efficiency, and GBP of a purity up to 99.8 % was produced. GBP liposomal gel (GLG) was prepared, and its stability, rheological property, release behavior and antibacterial activity were evaluated. According to the results, GLG had a small particle size and high homogeneity which was desirable for topical application, and it also manifested good physiochemical stability and suitable plastic flow property. In addition, GLG illustrated excellent inhibitory effect against Staphylococcus aureus , Escherichia coli , Candida albicans and Streptococcus pneumoniae , and the minimal inhibitory concentration was 25∼200 μg/mL. The systematic research provided a useful method for the biorefining of GBL.
研究领域:
植物化学
·
药剂学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2015
·
IF 2.516
Chang-Wei Zhang,Cheng-Zhang Wang,Ran Tao,Chuanjie Fang
Polyprenols are one of the most important lipid components in Ginkgo biloba leaves (GBLs). In this study, an enzymolysis-based ultrasound extraction procedure of GBL polyprenol (GBP) lipids was established, and the antioxidant activities of polyprenols and six kinds of polar fractions separated from GBL lipids via an optimal extraction procedure were studied. The results showed that the optimum conditions were an enzyme quantity of 0.5 g (the mass ratio of cellulase and pectinase was 1:2, and the enzyme activity was 60 U/mg), enzymolysis pH of 4.5, and temperature of ultrasound of 45 °C. Under these conditions, the yield of polyprenols was 0.80% ± 0.22%, which is 69.70% higher than that obtained by direct petroleum ether extraction. In addition, concentration c (content of polyprenols was 91.53%), fraction 3 (content of polyprenols was 43.28%), polyprenols (content of polyprenols >99%), and fraction 3 showed the strongest scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)), superoxide anion, and hydroxyl radicals with the corresponding IC 50 values of 236.9, 76.84, 80.23, and 113.9 μg/mL. It is inferred that GBL lipids with different concentrations of polyprenols could play different roles in the research and development of cosmetics and functional food.
研究领域:
植物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
Pharmaceutics
·
2022
·Vol. 14
·期 5
·923
·
IF 6.525
Ting Zhang,Xixi Li,Juewen Xu,Jingbao Shao,Meihong Ding,Senlin Shi
As a biopharmaceutics classification system (BCS) class IV drug, breviscapine (Bre) has low solubility in water, poor chemical stability, a short biological half-life and rapid removal from plasma. This paper prepared a Bre nanosuspension (Bre-NS) by an ultrasound-assisted anti-solvent precipitation method. Characterization of Bre-NS was studied using a Box–Behnken design concerning drug concentration in DMSO, an anti-solvent-to-solvent ratio, and sonication time. Under the optimized conditions of 170 mg/mL for the drug concentration, a 1:60 solvent-to-anti-solvent ratio, and a 9 min sonication time, the particle size of Bre-NS was 303.7 ± 7.3 nm, the polydispersity index was 0.178 ± 0.015, and the zeta potential was −31.10 ± 0.26 mV. Combined with the results from differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), and Fourier transform-infrared spectroscopy (FT-IR), the findings indicated that the crystal form and chemical structure of Bre-NS did not change during the entire process. The optimized formulation displayed good stability, increased solubility, and better in vitro release. Therefore, the results of this study can be a reference for the delivery system design of insoluble active components and effective parts in traditional Chinese medicine.Keywords:breviscapine;nanosuspension;Box–Behnken design
研究领域:
药学
·
药物递送系统
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Hong Shen,Changwei Zhang,Hao zhou,Chuan Li,Hua Yuan,Jianxin Jiang,Chengzhang Wang
Chlorophyll compounds (Chls) from Ginkgo biloba leaves (GBL) are non-toxic natural pigments characterized by highly conjugated molecules and special spectra, making them ideally potential photosensitizers (PSs) in photodynamics therapy (PDT). In this study, 4 Chls were isolated from Ginkgo biloba polyprenols (GBPPs) wastes by using silica gel column separation and high performance liquid chromatography (HPLC). In addition, two new chlorophyll derivatives were successfully synthesized with chlorin e6 as the primary raw material. To evaluate and identify superior chlorophyll PSs, Density Functional Theory (DFT) and Density Functional Theory (TD-DFT)were employed to decode the microscopic and spectral properties of Ginkgo biloba Chls (GBChls). The spectral absorption, optical stability and reactive oxygen species (ROS) production of GBChls were further analyzed through comparisons of their frontier molecular orbital layout, charge distribution and excitation energy properties. Our results show that about 63 % absorption in the Q band for 14 GBChls comes from the π⇢π* transition from the highest occupied orbital (HOMO) to the lowest unoccupied orbital (LUMO), indicating the favorable delocalization effect of these GBChls. In particular, those GBChls with the long chain of the phytyl group exhibited higher total electron energy and thus could significantly increase the light stability. However, the large spatial volume and hydrophobicity of these compounds with the phytyl group chain was unfavorable for cellular uptake, leading to low ROS in cells. The electronegativity of porphin macrocycles in GBChls positively correlates with the intracellular ROS production rate, meaning that the former likely promotes the energy transfer between PSs and molecular oxygen during type II photoreaction. Among the GBChls, chlorin e6 demonstrated the highest ROS yields in DMF, while purpurin 18 compounds displayed long-wavelength absorption (698 nm), strong Q-band absorption, good optical stability, and high intracellular ROS production. Our findings highlight that chlorin e6 and purpurin 18 are most potential PSs anticancer drugs and offer new research perspectives for the processing and utilization of GBL resources.
研究领域:
植物化学
·
药物化学
·
光动力疗法
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Ziyun Liu,Jianming Wang,Chen Yang,Zhuo Wang
Binary gels with various microstructures, including apricot kernel protein isolate-gellan gum gels with high freeze-thaw stability, were obtained by combining transglutaminase covalent cross-linking and gellan gum modification. Adding 0–0.1% gellan gum (w/w) can form continuous protein network structures dominated by covalent networks and disulfide bonds. In addition to covalent cross-linking, the increase in the strength of hydrophobic and electrostatic interactions at gellan gum concentrations below 0.2% (w/w) facilitated the formation of a bicontinuous network (AGG3). When excess gellan gum was added (0.03%, w/w), gellan gelation occurred in the system, leading to the formation of heterogeneous aggregated structures (AGG4). Confocal laser microscopy revealed a dense porous structure in the continuous protein network after freeze-thaw treatment, and AGG4 showed heterogeneous and loose aggregated microstructure, while the bicontinuous network structure was maintained. The water holding capacity of AGG3 was increased by 21.36% after freezing compared with the gels without gellan gum, and AGG3 maintained high hardness and springiness. Low-field nuclear magnetic resonance and magnetic resonance imaging showed that the bicontinuous network was more conducive to the tethering ability and uniform distribution of hydrogen protons than the continuous network structure of proteins. Therefore, AGG3 exhibited excellent freeze-thaw stability.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Zemin Li,Shuyan Xiang,Yin Pan,Li Fu
In this work, gold nanoparticles were prepared by reduction using Ginkgo biloba extract and modified with a glassy carbon electrode to investigate the electrochemical behavior of diclofenac. The effect of temperature on the synthesis of gold nanoparticles was investigated. The results showed that the best gold nanoparticles were synthesized at 180 °C. SEM characterized the prepared gold nanoparticles, and the size distribution was about 50~100 nm. During the electrochemical detection of diclofenac, two experimental parameters, the modification amount and the sweep rate were optimized, and a modification amount of 5 μL and a sweep rate of 100 mV/s were determined. The performance of the green synthesized gold nanoparticle modified electrode for the electrochemical detection of diclofenac was investigated using cyclic voltammetry with linear detection limits ranging from 5 to 600 μM and the lowest detection limit of 0.524 μM. In addition, the effect of impurity ions (molecules) on the electrochemical detection of diclofenac at gold nanoparticle-modified electrodes was investigated, and the results showed that none of the impurities measured affected the qualitative detection of diclofenac.
研究领域:
材料科学
·
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
Alexandria Engineering Journal
·
2024
·
IF 6.2
Tingting Yu,Ling Liu,Yutong Liu,Wanzhong Yin
Green synthesis of gold nanoparticles (AuNPs) using Ginkgo biloba leaf aqueous extract was optimized by response surface methodology to yield monodispersed, crystalline nanoparticles of 18 ± 4 nm diameter. Fourier transform infrared spectroscopy confirmed capping by flavonoids, terpenoids and polyphenols providing stability for over 6 months. The biosynthesized AuNPs exhibited promising in vitro dose and time-dependent antiproliferative effects against human nasopharyngeal carcinoma CNE1 cells with IC50 reducing from 65.7 to 39.2 μg/mL over 24–72 hrs. Distinct cytoplasmic damage was observed under microscope. Treatment with 50 μg/mL AuNPs for 48 hrs dramatically upregulated pro-apoptotic Bax, Caspase-3 and p53 by 8.7, 6.5 and 4.3 fold respectively while downregulating anti-apoptotic Bcl-2 by 11.2 fold, indicating apoptosis induction via mitochondrial intrinsic pathway. The green fabricated G. biloba AuNPs displayed preferential toxicity towards carcinoma cells compared to normal fibroblasts, indicating potential for targeted nasopharyngeal cancer therapy.
研究领域:
纳米医学
·
肿瘤学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Functional Foods
·
2024
·
IF 3.8
Yuyang Yao,Juer Liu,Qiming Miao,Xinyue Zhu,Lu Sun,Wei Hua,Na Zhang,Guangwei Huang,Roger Ruan,Yanling Cheng,Shengquan Mi
Controlling postprandial hyperglycemia is considered as a viable prophylactic treatment for type 2 diabetes mellitus. A major strategy in decreasing postprandial glucose involves inhibition of the carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase. The aim of this study was to evaluate the hypoglycemic potential of almond hull extract in vitro and in vivo. The in vitro results showed that, among the 0 %, 30 %, 50 % and 80 % ethanol extracts of almond hull, the 50 % ethanol extracts demonstrated the most significant inhibition of digestive enzymes. With in vivo tests, the blood glucose concentration in the control rats increased sharply near the peak glucose level in 15 min, while the peak glucose level of rats loading the almond hull extract occurred in 30 min. Therefore, almond hull extract can reduce postprandial blood glucose levels and delay the time to peak by about 15 min, providing a sustained and gradual release of energy.
研究领域:
药理学
·
代谢病研究
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR MODELING
·
2020
·Vol. 26
·期 8
·1-10
·
IF 1.346
Huang Xusheng,Zhang Wencheng,Wu Zeyu,Li Honghong,Yang Chengying,Ma Wanru,Hui Ailing,Zeng Qingmei,Xiong Baoyi,Xian Zhaojun
In this study, the preparation of molecularly imprinted polymers for bilobalide (BBMIPs) was successfully achieved by bulk polymerization with methacrylamide (MAM), trimethylolpropane triacrylate (TMPTA), and acetonitrile (ACN) as functional monomer, cross-linker, and solvent, respectively. After Gaussian software simulation and single factor experiments, the prepared MIPs with a molar ratio of 1:4:15 for BB-MAM-TMPTA were systematically characterized. The hydrogen bonding interaction between BB and MAM was confirmed by a combination of FTIR and NMR analysis. Thermal gravimetric analysis results displayed that MIPs have excellent thermal stability under high temperature. Additionally, the average pore size and surface area of MIPs were found to be higher than those of NIPs through nitrogen adsorption results. The results of static adsorption and kinetic adsorption suggested that the adsorption equilibrium concentration was 0.6 mg/mL and the equilibrium time was 5 h, and the Langmuir and pseudo-second-order kinetic models were proven to fit with static and kinetic adsorption behaviors, respectively. Meanwhile, the selective adsorption study revealed that MIPs show high adsorption and great selectivity towards BB in comparison with other substances having similarly structure. MIPs also possessed a good performance on reusability, maintaining a high recovery rate after being reused 5 times. The application experiment further indicated that MIPs can effectively separate BB from low purity samples. Therefore, the prepared MIPs had a great potential for BB separation.
研究领域:
分析化学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2023
·
IF 6
Qihui Dong,Huimin Wang,Rong Wu,Jun Cao,Fuliang Cao,Erzheng Su
For the quantification of ginkgolic acids (GAs) within ginkgo products, a very effective liquid–liquid microextraction pretreatment method based on hydrophobic deep eutectic solvent (HDES) was created. The HPLC detection conditions for GAs were optimized before establishing the pretreatment method. Then, the DES composition, DES volume, sample amount, extraction temperature and time were investigated to obtain the optimal pretreatment conditions. This developed method had satisfactory analytical performance, the linearity was r 2 = 0.9997, and its low limit of detection (LOD) was 0.81 mg/kg. The recovery value ranged from 95.10 % to 98.75 %. When compared with the Ch.P.2010 and EP10.0 recommended methods, this developed method gave a higher efficiency and showed obvious advantages. Furthermore, the developed method was effectively used to identify GAs in a variety of Ginkgo biloba medications, Ginkgo biloba leaves from various cultivars and collection times. These results highlighted the superiorities of the HDES-based liquid–liquid microextraction pretreatment technique for the measurement of GAs, providing insights for future development of efficient methods in active ingredients analysis.
研究领域:
分析化学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER INTERNATIONAL
·
2021
·Vol. 71
·期 3
·301-309
·
IF 2.99
Xusheng Huang,Qingsong Liu,Wencheng Zhang,Zeyu Wu,Yunchun Wang,Jianwen Li,Baoyi Xiong,Zhaojun Xian
The preparation of raw materials is an important prerequisite for studying the function of natural active ingredients. Here, the preparation of MWCNTs/ZIF-67@MIPs based on surface molecular imprinting technology for the separation of bilobalide (BB) from real samples was proposed. The successful preparation of MWCNTs/ZIF-67@MIPs was demonstrated using several techniques. Adsorption equilibrium was achieved when the time was 4 h, and the rate of adsorption calculated from the dynamic adsorption results was 14.2 μg mL –1 h –1 . The maximum adsorption amount for MWCNTs/ZIF-67@MIPs of BB was calculated to be 59.2 mg g –1 , which was more than three times that for MWCNTs/ZIF-67@NIPs (23.1 mg g –1 ), and the Freundlich isotherm model and pseudo-second-order kinetic model were more suitable for the explanation of isothermal and kinetic adsorption behaviors, respectively. The results of selective adsorption indicated that MWCNTs/ZIF-67@MIPs show excellent selectivity and specificity for BB compared to structurally similar compounds such as ginkgolides A, B and C, with an excellent imprinting factor (2.83). The results of reuse studies indicated that MWCNTs/ZIF-67@MIPs exhibited great reproducibility with a recovery rate of 70.8% after repeated use for five times. Finally, the developed MWCNTs/ZIF-67@MIPs were used as a solid-phase extraction sorbent to directly separate BB from treated gingko leaf extract and crude extract of BB, and the results suggested that the purity of BB in the two samples was increased by about 30%, and the recoveries reached 68.34% and 71.54%, respectively. Meanwhile, the separation effect of MWCNTs/ZIF-67@MIPs on BB was better than that of BBMIP prepared by bulk polymerization. © 2021 Society of Industrial Chemistry.
研究领域:
分析化学
·
天然产物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 19
·5740
·
IF 4.412
Kai Zhang,Yanchao Liu,Sheng Cui,Yimin Xie
To elucidate the chemical linkages between lignin and carbohydrates in ginkgo cell walls,13C-2H-enriched cell wall-dehydrogenation polymers (CW-DHP) were selectively prepared with cambial tissue fromGinkgo bilobaL. by feeding D-glucose-[6-2H2], coniferin-[α-13C], and phenylalanine ammonia-lyase (PAL) inhibitor. The abundant detection of13C and2H confirmed that D-glucose-[6-2H2] and coniferin-[α-13C] were involved in the normal metabolism of ginkgo cambial cells that had been effectively labelled with dual isotopes. In the ginkgo CW-DHP, ketal and ether linkages were formed between the C-α of lignin side chains and carbohydrates, as revealed by solid state CP/MAS13C-NMR differential spectroscopy. Furthermore, the DMSO/TBAH ionic liquids system was used to fractionate the ball-milled CW-DHP into three lignin-carbohydrate complex (LCC) fractions: glucan–lignin complex (GL), glucomannan–lignin complex (GML), and xylan–lignin complex (XL). The XRD determination indicated that the cellulose type I of the GL was converted into cellulose type II during the separation process. The molecular weight was in the order of Ac-GL > Ac-GML > XL. The13C-NMR and1H-NMR differential spectroscopy of13C-2H-enriched GL fraction indicated that lignin was linked with cellulose C-6 by benzyl ether linkages. It was also found that there were benzyl ether linkages between the lignin side chain C-α and glucomannan C-6 in the13C-2H-enriched GML fraction. The formation of ketal linkages between the C-α of lignin and xylan was confirmed in the13C-2H-enriched XL fraction.Keywords:lignin–carbohydrate complexes;CW-DHP;isotope labelling;ginkgo;NMR
研究领域:
植物科学
·
化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）