FOOD CHEMISTRY
·
2025
·
IF 9.8
Yu-Jie Shi,Fan- Gong,Chang-Ying Hu,Zhi-Wei Wang,Jun-Peng Hu,Yan-Ping Xian
Bisphenol A diglycidyl ether (BADGE) from the epoxy coating can migrate to food and react with the protein, but the safety of these adducts formed is unknown, besides, after being consumed in large quantities, BADGE may also combine with other proteins supplemented, posing a potential hazard to human health. The binding mechanism and the possible binding products of BADGE and protein were explored by a series of liquid chromatograph-tandem mass spectrometry (LC-MS) methods. Results showed BADGE can modify a variety of amino acid residues with two modification modes, of which the largest number of modifications were cysteine (Cys) and lysine (Lys). Some adducts formed had shown potential toxicity by in-silico toxicity evaluation and needed to be concerned, which provided a new perspective on the safety of epoxy coating. The molecular docking simulation revealed that hydrophobicity and hydrogen bond interactions are the main intermolecular forces in complex formation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shan-Cheng Yi,Juan Wu,Jia-Qing Wang,Xin-Hui Chen,Man-Qun Wang
Odorant binding proteins (OBPs) are involved in odorant discrimination and act as the first filter in the peripheral olfactory system. Previous studies have shown that BhorOBP29 is potentially involved in olfactory perception in an important wood-boring pest Batocera horsfieldi (Hope) (Coleoptera: Cerambycidae), however, its function remains unclear. Here, we investigated the ligand-binding profiles of recombinant BhorOBP29 with 22 compounds from its host plant using fluorescence competitive binding assays and fluorescence quenching assays. The results showed that BhorOBP29 could bind to five ligands relying mainly on hydrophobic interactions. Molecular docking analysis indicated that residues Ile48, Leu51, Met52, Trp57, Asn105, and Val119 were extensively involved in the interactions between BhorOBP29 and the five ligands. Furthermore, the site-directed mutagenesis analysis revealed that Leu51 and Met52 residues were indispensable for BhorOBP29-ligands binding. Finally, electroantennogram (EAG) assays confirmed that hexanal, (−)-limonene, and 2-methylbutyraldehyde elicited a concentration-dependent EAG response with a maximum at the concentration of 1/10 v /v. These findings suggest that BhorOBP29 may play a significant role in the perception of host plant volatiles by B. horsfieldi . This study may help to discover novel behavioral regulation and environmentally friendly strategies for controlling B. horsfieldi in the future.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Additive Manufacturing
·
2024
·
IF 10.3
Yixian Wang,Xin Xu,Desheng Liu,Jiayu Wu,Xingxing Yang,Bin Zhu,Chufeng Sun,Pan Jiang,Xiaolong Wang
Owing to flexible structural design and rapid prototyping manufacturing, 3D printable hydrogels with solid-like and fluid-like characters have emerged as a promising candidate for biomedical engineering, soft robotics, and flexible electronics. However, existing 3D printing of hydrogel place substantial constraints on hydrogel high-fidelity of the printed hydrogel complex structures due to the shape deformation and collapse caused by water evaporation. Herein, we propose a mixed solvent printing strategy followed with water dialysis to achieve vat photopolymerization 3D printing of high-fidelity hydrogel structure by restraining water evaporation. Concretely, dimethyl sulfoxide (DMSO) is introduced into the hydrogel ink as co-solvent to improve the anti-dehydration performance during 3D printing. The as-prepared gels are then transformed into hydrogels with good fidelity and excellent performance through solvent exchange and water dialysis. Enabled by these characteristics, various functional devices with complex structures are demonstrated with the present high-fidelity 3D printing of diverse hydrogels. The solvent assisted high-fidelity hydrogel 3D printing protocol combining with the compatibility to various high-performance hydrogels is believed to be promising for wearable devices, wound dressings, electronic skin, and intelligent robots.
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2024
·
IF 13
Jiahui Zhu,Ran Wei,Guang Hu,Hui Wang,Wenbin Wang,Haiqiang Wang,Jidan Huang,Yu Wang,Yujing Li,Huan Meng
Chondrosarcoma(CS), a prevalent primary malignant bone tumor, frequently exhibits chemotherapy resistance attributed to upregulated anti-apoptosis pathways such as the Bcl-2 family. In this manuscript, a new strategy is presented to augment chemosensitivity and mitigate systemic toxicity by harnessing a nano-enabled drug delivery hydrogel platform. The platform utilizes “PLGA-PEG-PLGA”, an amphiphilic triblock copolymer combining hydrophilic polyethylene glycol (PEG) and hydrophobic polylactide glycolide (PLGA) blocks, renowned for its properties conducive to crafting a biodegradable, temperature-sensitive hydrogel. This platform is tailored to encapsulate a ratiometrically designed dual-loaded liposomes containing a first-line chemo option for CS, Doxorubicin (Dox), plus a calculated amount of small molecule inhibitor for anti-apoptotic Bcl-2 pathway, ABT-737. In vitro and in vivo evaluations demonstrate successful Bcl-2 suppression, resulting in the restoration of Dox sensitivity, evident through impeded tumor growth and amplified necrosis rates at the tumor site. This delivery system showcases remarkable thermal responsiveness, injectability, and biodegradability, all finely aligned with the clinical demands of CS treatment. Collectively, this study introduces a transformative avenue for tackling drug resistance in CS chemotherapy, offering significant clinical potential.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sustainable Chemistry and Pharmacy
·
2024
·
IF 5.5
Xing Zhou,Ping-Yue Wang,Chen-Xi He,En-Jang Liu,Xiao-Hui Yao,Dong-Yang Zhang,Tao Chen
Recently, continuous-fluid catalysis technology has been widely used in biocatalysis. However, the poor stability of the reactor-supported enzyme and expensive manufacturing cost of the reactor restrict its use. Herein, a wood-based bioreactor, β-glucosidase@ zeolitic imidazolate framework-8@delignified wood (β-Glu@ZIF-8@DW), with attractive features was developed. The naturally oriented channels of the xylem-based reactor enable high throughput. The interference of the fluids through the porous inner walls in the directional channels of the xylem-based reactor considerably increased the probability of contact between the substrate and enzyme. Resveratrol with multifaceted biological activities exists in many plants, including Mulberry (Morus spp., Moraceae) and Camellia sinensis and Polygonum cuspidatum (P. cuspidatum) . However, it mainly presents in its glycosylated form (polydatin) rather than as free resveratrol in plant. To improve the acquisition of resveratrol, continuous convert polydatin into resveratrol by a bioreactor is a good strategy. The conversion rate of polydatin using the as-prepared reactor reached 98.98% in 2 h. The uniform distribution of β-Glu@ZIF-8 in the channel increased the stability of the bioreactor with an 80.28% conversion rate after seven cycles. The reactor provided an excellent strategy for continuous catalysis, which can be used for industrial production with low synthesis costs and high mass transfer efficiency. Therefore, it has great potential for commercial applications.
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2024
·
IF 3.7
Dandan Ren,Jiale Xie,Tianle Chen,Haibin Qu,Xingchu Gong
ABSTRACT The current methods used to industrially produce sinomenine hydrochloride involve several issues, including high solvent toxicity, long process flow, and low atomic utilization efficiency, and the greenness scores of the processes are below 65 points. To solve these problems, a new process using anisole as the extractant was proposed. Anisole exhibits high selectivity for sinomenine and can be connected to the subsequent water-washing steps. After alkalization of the medicinal material, heating extraction, water washing, and acidification crystallization were carried out. The process was modeled and optimized. The design space was constructed. The recommended operating ranges for the critical process parameters were 3.0–4.0 h for alkalization time, 60.0–80.0 °C for extraction temperature, 2.0–3.0 (volume ratio) for washing solution amount, and 2.0–2.4 mol·L −1 for hydrochloric acid concentration. The new process shows good robustness because different batches of medicinal materials did not greatly impact crystal purity or sinomenine transfer rate. The sinomenine transfer rate was about 20% higher than that of industrial processes. The greenness score increased to 90 points since the novel process proposed in this research solves the problems of long process flow, high solvent toxicity, and poor atomic economy, better aligning with the concept of green chemistry.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 12
·1-10
·
IF 5.3
Peng Tao,Liang Zhanwei,Si Jihao,Huang Lu,Ye Hua,Li Xiaoqing,Sun Miao,Xie Jie,Dai Xinhua
Novel green-emitting fluorescent microspheres (GreFMPs) were assembled by loading highly luminescent gold nanoclusters (Arg/ATT/AuNCs) on dendritic mesoporous silica nanoparticles (DMSNs) via PEI-mediated electrostatic adsorption. The fluorescence microspheres exhibit excellent monodispersion with average diameters about (254.5 ± 34.6 nm). Compared with free Arg/ATT/AuNCs, the GreFMPs have similar fluorescent properties and biocompatibility but superior environmental tolerance. Subsequently, an immunochromatographic assay based on GreFMPs (GreFMP-ICA) has been successfully developed, which is highly selective toward alpha fetoprotein (AFP) in human serum. Under the optimal parameters, there is a good linear range between 0.5 and 160.0 ng/mL, the limit of detection was found to be about 0.5 ng/mL, and the recovery and relative standard deviation are 81.0 ~ 100.6% and 3.5 ~ 16.6%, respectively. These results manifested that the GreFMPs are beneficial for the rational design of the ICA platform, and the proposed GreFMP-ICA has the potential to screen AFP in clinical applications. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Qiaolin Tang,Feng Zhu,Yanqi Li,Sijie Yin,Yunbo Xu,Huicheng Yan,Ming Kang,Guanjun Chang
In the present study, a novel silane coupling agent, designated INSi, was synthesized via a facile synthetic route, incorporating indole-functional moieties. This agent was further employed for the surface modification of homemade silica nanomicrospheres (SMPs). The ensuing nanomicrosphere composite, denoted as SiO2@IN, exemplified pronounced interfacial π–π interactions. Optimization of the reaction conditions was conducted using the response surface optimization technique. Subsequent validation of interfacial π–π interactions was accomplished through a synergistic approach, integrating theoretical calculations and comprehensive analyses of spectral and morphological attributes exhibited by the SiO2@IN.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Dongdong Xu,Zongli Li,Zhennan Deng,Xin Nie,Yihuai Pan,Gu Cheng
Degradation profiles are critical for the optimal application of electrospun polymer nanofibers in tissue regeneration, wound healing, and drug delivery systems. In this study, natural and synthetic polymers and their composites were subjected to in vivo transplantation and in vitro treatment with lipases, macrophages, and acetic acid to evaluate their degradation patterns. The effects of environmental stimulation, surface wettability, and polymer components on the degradation profiles of the electrospinning poly(ε-caprolactone)/silk fibroin (PCL/SF) nanofibers were first evaluated. In vivo degradation study demonstrated that bulk degradation, characterized by the transition from microfibers to nanofibers, and surface erosion, characterized by fusion between the microfibers or direct erosion from both ends of the microfibers, occurred in the electrospun membranes; however, bulk degradation dominated their overall degradation. Furthermore, the degradation rates of the electrospun PCL/SF membranes varied according to the composition, morphology, and surface wettability of the composite membranes. After the incorporation of silk fibroin (SF), the degradation rate of the SF/PCL composite membranes was faster, accompanied by larger values of weight loss and molecular weight ( Mw ) loss when compared with that of the pure poly(ε-caprolactone) (PCL) membrane, indicating a close relationship between degradation rate and hydrophilicity of the electrospinning membranes. The in vitro experimental results demonstrated that enzymes and oxidation partially resulted in the surface erosion of the PCL/SF microfibers. Consequently, bulk degradation and surface erosion coordinated with each other to enhance the hydrophilicity of the electrospinning membranes and accelerate the in vivo degradation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Yang Zhang,Lingwei Gao,Juhong Zhan,Erzhuo Zhao,Huijiao Wang,Chunrong Wang,Yujue Wang
In this study, degradation of the neonicotinoid insecticide imidacloprid (IMI) by ozonation and the E-peroxone process was investigated. Results show that IMI is an O 3 -refractory compound with second-order rate constants for the reaction with O 3 and • OH ( k O3,IMI and k •OH,IMI ) being 3.9 and 3.95 × 10 9 M −1 s −1 at pH 7, respectively. Due to its low O 3 reactivity, IMI was only abated by < 20 % in a selected groundwater during ozonation with typical ozone doses applied in water treatment (1–5 mg/L). In comparison, the E-peroxone process considerably enhanced IMI removal efficiency to > 80 % under similar reaction conditions. During both ozonation and the E-peroxone process, IMI was mainly abated by • OH oxidation. The reaction mechanism of IMI with • OH was investigated with quantum computations, which show that H-abstraction at H23 site was the most favorable pathway. Sixteen transformation products (TPs) generated from IMI degradation were identified using various chromatographic and mass-spectrometric techniques. The acute and chronic toxicities of IMI and its TPs to different aquatic organisms were evaluated with Microtox bioassay and the ECOSAR program, which indicate that some TPs (e.g., TP 2 –TP 5 and TP 7 –TP 9 ) are even more toxic than IMI to aquatic organisms (e.g., daphnia). The results of this study suggest that IMI along with its TPs should be sufficiently abated during water treatment, and the E-peroxone process provides an efficient option to upgrade conventional ozonation for enhanced IMI abatement.
✓ 已验证
产品关联
DOI
高影响因子
Biomolecules
·
2024
·Vol. 14
·期 8
·907
·
IF 4.8
Yusu Wang,Ying Zong,Weijia Chen,Naichao Diao,Quanmin Zhao,Chunyi Li,Boyin Jia,Miao Zhang,Jianming Li,Yan Zhao,Rui Du,Zhongmei He
Due to the limited supply of autologous bone grafts, there is a need to develop more bone matrix materials to repair bone defects. Xenograft bone is expected to be used for clinical treatment due to its exact structural similarity to natural bone and its high biocompatibility. In this study, decellularized antler cancellous bone matrix (DACB) was first prepared, and then the extent of decellularization of DACB was verified by histological staining, which demonstrated that it retained the extracellular matrix (ECM). The bioactivity of DACB was assessed using C3H10T1/2 cells, revealing that DACB enhanced cell proliferation and facilitated cell adhesion and osteogenic differentiation. When evaluated by implanting DACB into nude mice, there were no signs of necrosis or inflammation in the epidermal tissues. The bone repair effect of DACB was verified in vivo using sika deer during the antler growth period as an animal model, and the molecular mechanisms of bone repair were further evaluated by transcriptomic analysis of the regenerated tissues. Our findings suggest that the low immunogenicity of DACB enhances the production of bone extracellular matrix components, leading to effective osseointegration between bone and DACB. This study provides a new reference for solving bone defects.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER
·
2024
·
IF 4.1
Xiaoyan Wang,Pengsheng Jing,Jinmei Du,Guowei Xiao,Yang Jiang,Dongyan Shao,Yanmei Qu,Changhai Xu
Traditional polyurethane fibers (CPUF) are widely used in the preparation of blend clothing in order to improve the elasticity and comfort of fabrics. However, there has been a problem of low acid dye adsorption for CPUF, resulting in light hue of dyed CPUF. In this work, a poly (HMDI-BHOPA) was prepared with dicyclohexylmethane 4,4′-diisocyanate (HMDI) and 1,4-(2-hydroxyethyl) piperazine (BHOPA), and then added into the CPUF spinning solution for the preparation of modified polyurethane fibers (BPUFs) through dry-spinning technology. Tertiary amine groups were introduced after the addition of poly (HMDI-BHOPA), which could be protonated at acidic conditions to form binding sites for acid dyes, further enhancing the adsorption capacity of the fibers. Nuclear magnetic resonance spectroscopy ( 1 H and 13 C NMR) was used to confirm the structure of poly (HMDI-BHOPA). The results of differential scanning calorimetry (DSC) and thermogravimetry analysis (TGA) demonstrated that the addition of poly (HMDI-BHOPA) had little effect on the glass transition temperature and thermal stability of BPUFs. The addition of poly (HMDI-BHOPA) affected the ordered arrangement of hard segments and reduced crystallization enthalpy of hard and soft segments in polyurethane. Hence, the elastic recovery of BPUFs showed little change, and the elongation at break and break strength decreased compared to CPUF. Zeta potential testing results showed that BPUFs was protonated under acidic conditions. Dyeing results proved the enhancement of dyeability of BPUFs. The dyeing kinetics and thermodynamics of acid dyes on BPUFs were studied to investigate the dyeing mechanism. From the dyeing kinetics results, both CPUF and BPUFs fitted Elovich model. From dyeing thermodynamics results, Langmuir model showed a better applicability for BPUFs at a low pH and temperature condition, while Freundlich model fitted better for CPUF.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）