计算溶液所需的质量、体积或浓度。
黏液卡红染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
黏液物质染色有多种方法，如AB-PAS染色、黏液HID-AB染色、标准阿利新蓝染色等，以上方法大多是利用阿利新蓝 (Alcian)属于阳离子染料可与酸性基团结合，也即阿尔辛蓝与组织内含有的阴离子基团如羧基和硫酸根形成不溶性复合物这一原理，然而上述方法不是专门显示中性黏液物质的。
黏液卡红染色主要用于显示中性黏液物质，也可显示酸性粘液物质和新型隐球菌，特别适用于显示胃和上皮的黏液，临床上多用于分泌黏液的上皮癌的鉴别。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
| M1508531 | Component | 3×50 mL | Storage |
| M1508531A | LM苏木素染色液 | 50 mL | RT. Store in the dark. |
| M1508531B | 酸性乙醇分化液 | 50 mL | RT. |
| M1508531C | 黏液卡红原液 | 10 mL | 2-8℃. Store in the dark. |
自备材料：
1、蒸馏水、系列乙醇/二甲苯或浸蜡脱蜡透明液、中性树胶
2、显微镜、载玻片
操作步骤(仅供参考)：
1、切片二甲苯或脱蜡透明液脱蜡至水，细胞涂片应固定后水洗。
2、LM苏木素染色液染核3-5min，如为细胞涂片染色1-3min即可，流水冲洗5min。
3、酸性乙醇分化液分化2-5s，流水冲洗返蓝。
4、在上述操作过程中提前配制黏液卡红工作液，即按黏液卡红原液:蒸馏水=1:4的比例配制；入黏液卡红工作液复染30min-数小时，应根据室温来调整时间，如为细胞涂片染色20-30min即可。
5、流水冲洗。
6、梯度乙醇脱水，二甲苯或-脱蜡透明液透明，中性树胶封片。
染色结果:
黏液物质: 红色
细胞核: 蓝色
注意事项：
1、固定液采用10%中性福尔马林。
2、LM苏木素染色液染核时应淡染。
3、黏液卡红工作液易挥发、易沉淀，应用立式染色缸染色。如产生沉淀，应过滤后使用。
4、黏液卡红工作液染色后，如果想做对比染色，可采用低浓度淡绿快速染色数秒。
5、乙醇脱水过程同时具有分化作用，应注意控制时间。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
7、试剂开封后请尽快使用，以防影响后续实验效果。
There are various methods for staining mucous substances, such as AB-PAS staining, mucin HID-AB staining, standard Alcian blue staining, etc. Most of these methods utilize the principle that Alcian blue, a cationic dye, can bind to acidic groups-specifically, Alcian blue forms insoluble complexes with anionic groups (e.g., carboxyl and sulfate groups) contained in tissues. However, the aforementioned methods are not designed specifically for demonstrating neutral mucous substances.
Mucicarmine staining is mainly used to display neutral mucous substances; it can also stain acidic mucous substances and Cryptococcus neoformans. It is particularly suitable for showing mucus in the stomach and epithelium, and is clinically widely used for the differentiation of mucus-secreting epithelial carcinomas. This reagent is for research use only and not intended for clinical diagnosis or other purposes.
| M1508531 | Component | 3×50 mL | Storage |
| M1508531A | LM Hematoxylin Staining Solution | 50 mL | RT. Store in the dark. |
| M1508531B | Acidic Ethanol Differentiating Solution | 50 mL | RT. |
| M1508531C | Mucicarmine Stock Solution | 10 mL | 2-8℃. Store in the dark. |
Materials to Be Prepared by the User
1. Distilled water, graded ethanol/xylene or dewaxing-clearing solution, neutral gum
2. Microscope, glass slides
Operating Procedures (For Reference Only)
1. Dewax sections to water using xylene or dewaxing-clearing solution; cell smears should be fixed before washing with water.
2. Stain cell nuclei with LM Hematoxylin Stain Solution for 3-5 minutes (for cell smears, 1-3 minutes is sufficient), followed by rinsing with running water for 5 minutes.
3. Differentiate with Acidic Ethanol Differentiating Solution for 2-5 seconds, then rinse with running water for bluing.
4. Prepare the mucicarmine working solution in advance during the above steps at a ratio of mucicarmine stock solution: distilled water=1:4. Counterstain the samples with the mucicarmine working solution for 30 minutes to several hours; adjust the duration according to room temperature (for cell smears, 20-30 minutes is adequate).
5. Rinse with running water.
6. Dehydrate with graded ethanol, clear with xylene or dewaxing-clearing solution, and mount with neutral gum.
Staining Results
Mucous substances: Red
Cell nuclei: Blue
Precautions
1. Use 10% neutral formalin as the fixative.
2. Stain cell nuclei lightly with LM Hematoxylin Stain Solution.
3. The mucicarmine working solution is volatile and prone to precipitation; use a vertical staining jar for staining. If precipitation occurs, filter the solution before use.
4. For counterstaining after mucicarmine working solution staining, rapid staining with low-concentration light green for a few seconds is recommended.
5. The ethanol dehydration process also has a differentiating effect; strictly control the dehydration time.
6. For your safety and health, wear a lab coat and disposable gloves during operation.
7. Use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
规格
规格或纯度
BioReagent,用于显微镜,生物染色剂
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Mucicarmine Staining Solution
储存条件
2-8°C储存,避光,室温
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131587 |
分析证书 | M1508531 |
| ZJ26F0131586 |
分析证书 | M1508531 |
| ZJ26F0131585 |
分析证书 | M1508531 |
技术文档和文章
Application Differences Among Highman, Bennhold, Puchtler, and Modified Methods in Congo Red Staining for Amyloid
目录： 技术文章
Identification of Tissue Components, Method Selection, and Result Interpretation in Connective Tissue Staining
目录： 技术文章
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