May-Grunwald-Giemsa Stain 经常用于组织切片、血液和细胞涂片、细菌、染色体显带、原生动物寄生虫等染色，尤其适用于胃幽门螺杆菌染色。胃幽门螺杆菌 (HelicobacterPyloric) 又称胃幽门弯曲菌 (CampylobacterPyloric)，现已证实这种细菌与慢性胃炎和消化性溃疡有密切关系，胃幽门螺杆菌一般呈弧形、S形或海鸥状，有时可见3～4个弯曲呈螺旋状，常呈鱼群状分布；该菌多见于胃黏膜表面上皮与黏膜层之间，并贴近表面上皮细胞，部分进入上皮细胞胞质内，胃小凹和黏膜浅层腺腔内亦有此菌。
幽门螺旋杆菌染色的方法主要有亚甲蓝法、硝酸银法、迈格林华-姬姆萨法 (May-Grunwald-Giemsa，MGG法)、碱性品红法等，硝酸银法对比清楚，染片可以长期保存，但操作较为麻烦耗时，其他方法较为简便、但染片容易褪色。切片经May-Grunwald染色后胃幽门螺旋杆菌呈蓝色，胶原纤维呈红色，红细胞呈绿色，胃黏膜上皮呈淡蓝色，细胞核呈深蓝色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
M1510448
| Component
| 2×100mL
| Storage
|
M1510448A
| May-Grunwald Stain
| 100mL
| RT
|
M1510448B
| MG磷酸盐缓冲液
| 100mL
| RT |
自备材料：
1、二甲苯或浸蜡脱蜡透明液、10%福尔马林固定液
2、蒸馏水、梯度乙醇、中性树胶
操作步骤(仅供参考)：
1、组织固定于10%福尔马林，常规脱水包埋。
2、切片厚4μm，常规二甲苯或浸蜡脱蜡透明液脱蜡至水，蒸馏水洗1次。
3、把切片周围水分抹干，滴入May-GrunwaldStain工作液数滴染10～15min，倾去染液。
4、无水乙醇快速洗去余液，稍烤干。
5、二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
胃幽门螺旋杆菌，细胞核，胃黏膜上皮
| 蓝色
|
胶原纤维
| 红色
|
红细胞
| 绿色
|
注意事项：
1、May-Grunwald工作液须于临用前配制，不易保存。
2、最后的无水乙醇要速洗，否则容易脱色。
3、胃幽门螺旋杆菌仅用 Giemsa Stain 工作液染色也可以显示出来，也较清晰；其最佳效果应为 May-Grunwald Stain 和 Giemsa Stain合用，参考阿拉丁 幽门螺旋杆菌染色液 (MGG法) (H1510399)。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用，以防影响后续实验效果。
May-Grunwald-Giemsa Stain is commonly used for staining tissue sections, blood and cell smears, bacteria, chromosome banding, and protozoan parasites, and is particularly suitable for staining Helicobacter pylori. Helicobacter pylori, also known as Campylobacter pyloric, has been confirmed to be closely associated with chronic gastritis and peptic ulcers. Helicobacter pylori is generally arc-shaped, S-shaped or seagull-shaped; sometimes it shows 3–4 curves to form a spiral shape, and is often distributed in a fish-school-like pattern. The bacterium is mostly found between the surface epithelium and the mucosal layer of the gastric mucosa, adhering closely to the surface epithelial cells, with some entering the cytoplasm of epithelial cells. It can also be detected in gastric pits and the glandular lumens of the superficial mucosa.
The main staining methods for Helicobacter pylori include the methylene blue method, silver nitrate method, May-Grunwald-Giemsa method (MGG method), and basic fuchsin method. The silver nitrate method yields clear contrast and stained slides can be preserved for a long time, but the operation is cumbersome and time-consuming. Other methods are relatively simple, yet the stained slides are prone to fading. After staining sections with May-Grunwald Stain, Helicobacter pylori appears blue, collagen fibers appear red, red blood cells appear green, gastric mucosal epithelium appears light blue, and cell nuclei appear dark blue. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
M1510448
| Component
| 2×100mL
| Storage
|
M1510448A
| May-Grunwald Stain
| 100mL
| RT
|
M1510448B
| MG Phosphate Buffer Solution
| 100mL
| RT |
Self-prepared Materials:
1. Xylene or paraffin-embedded dewaxing and clearing solution, 10% formalin fixative
2. Distilled water, graded ethanol, neutral balsam
Operating Procedures (For Reference Only):
1. Fix the tissue in 10% formalin, then perform routine dehydration and embedding.
2. Cut the tissue into sections with a thickness of 4 μm, conduct routine dewaxing to water using xylene or paraffin-embedded dewaxing and clearing solution, and rinse once with distilled water.
3. Wipe off the water around the sections, add several drops of May-Grunwald Stain working solution, stain for 10–15 minutes, and pour off the staining solution.
4. Quickly rinse off the residual solution with anhydrous ethanol and dry slightly by heating.
5. Clear the sections with xylene or dewaxing and clearing solution, then mount the sections with neutral balsam.
Staining Results:
Helicobacter pylori, Cell nucleus, Gastric mucosal epithelium
| Blue
|
Collagen fibers
| Red
|
Red blood cells
| Green |
Precautions:
1. May-Grunwald working solution must be prepared immediately before use and is not suitable for long-term storage.
2. The final rinsing step with anhydrous ethanol should be performed quickly; otherwise, the stain is prone to fading.
3. Helicobacter pylori can also be clearly visualized by staining with Giemsa Stain working solution alone. For optimal staining results, the combined use of May-Grunwald Stain and Giemsa Stain is recommended (refer to Aladdin Helicobacter pylori Staining Solution (MGG Method) (H1510399)).
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
5. Please use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.
ZJ26F0433837
危险品化学品经营许可证（带存储）