计算溶液所需的质量、体积或浓度。
Mallory PTAH染色液 (自然氧化法)
有货
为什么选择此级别
BioReagent,生物染料级,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
肌纤维(Musclefiber)属于肌组织成分，由肌细胞组成，根据形态和功能特点，肌纤维可以分为平滑肌(又称横纹肌)、骨骼肌、心肌。肌纤维染色的方法有很多种，如丽春红法、苯胺蓝法、钨磷钨酸苏木素法等，最初发明磷钨酸苏木素染色液时，Mallory的PTAH方法中有多种钨磷酸苏木素方法，1900年左右Mallory将磷钨酸水溶液和苏木素染液联用，发现该法对于肌纤维染色较好，现在广泛使用的即为Mallory磷钨酸苏木素染色液(PTAH自然氧化法)。苏木素可用PTAH化学氧化法，但有效期较短且染色力易下降，尽管自然氧化耗费时间，但制得的苏木素可用2年以上，染色力也不易丢失，是较为理想的染色液，适用于CNS、一般组织结构以及所有标准固定液固定的组织，染色时间依配制方法、所用固定液和所显示的组织结构而异。
Mallory PTAH染色液 (自然氧化法) 又称Mallory磷钨酸苏木素染色液，为自然成熟的染液，染色力较好，保存时间长，多用于显示横纹肌的横纹，临床上应用该法对横纹肌肉瘤进行诊断，横纹肌肉瘤的组织学形态变化多样，与未分化的间胚叶肿瘤很难鉴别；采用磷钨酸苏木素染色后，如果在瘤细胞胞质内发现蓝色横纹，则可以证明该肿瘤是呈横纹肌分化；该染色液也可以对炎症渗出的纤维素、DIC的毛细血管中纤维素以及神经病理等方面进行染色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、10%福尔马林固定液、酸化高锰酸钾溶液、草酸溶液、蒸馏水、系列乙醇
2、二甲苯或环保脱蜡透明液、中性树胶
3、光学显微镜
操作步骤(仅供参考)：
1、组织固定于10%福尔马林固定液中，常规脱水包埋。
2、石蜡切片厚4μm，常规二甲苯或脱蜡透明液脱蜡至水。
3、入酸化高锰酸钾氧化5min，稍水洗。
4、入草酸溶液漂白1~2min，自来水冲洗2min，用蒸馏水洗1次。
5、入Mallory PTAH染色液 (自然氧化法)浸染(加盖)24~48h。
6、取出切片，直接用95%的乙醇迅速洗去多余染液。
7、常规脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
横纹肌的横纹、纤维素、胞核、红细胞和神经胶质纤维：深蓝色
胶原纤维、软骨基质：棕红色
粗的弹性纤维：紫色
注意事项：
1、在染色时若显示横纹的蓝色不够或横纹呈鲜红色，说明氧化时间不足或者可能是已过度氧化，需要重新换染液或配制新液。
2、Mallory PTAH染色液染色后不要水洗，在95%乙醇洗时也要迅速，因为水洗或乙醇洗的时间稍长，都可以洗脱磷钨酸苏木素所着染的颜色。
3、Mallory磷钨酸苏木素为进行性染色，不要过染，在染色24小时后，可取出在显微镜下观察着色程度。4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Muscle fibers are a component of muscle tissue and are composed of muscle cells. According to their morphological and functional characteristics, muscle fibers can be classified into smooth muscle, skeletal muscle (also known as striated muscle), and cardiac muscle. There are many methods for staining muscle fibers, such as the Ponceau method, Aniline Blue method, and Phosphotungstic Acid Hematoxylin method. When the phosphotungstic acid hematoxylin staining solution was first developed, several versions existed within Mallory’s PTAH method. Around 1900, Mallory combined aqueous phosphotungstic acid solution with hematoxylin staining solution and found it produced excellent staining of muscle fibers. The Mallory Phosphotungstic Acid Hematoxylin Staining Solution (PTAH, natural oxidation method) is now widely used. Hematoxylin can also be prepared by the PTAH chemical oxidation method, but it has a shorter shelf life and its staining ability tends to decline. Although natural oxidation takes longer, the resulting hematoxylin can be stored for more than 2 years with stable staining capacity, making it an ideal staining solution. It is suitable for central nervous system (CNS) tissues, general histological structures, and tissues fixed with all standard fixatives. Staining time varies according to the preparation method, fixative used, and histological structure to be demonstrated.
Mallory PTAH Staining Solution (Natural Oxidation Method), also known as Mallory Phosphotungstic Acid Hematoxylin Staining Solution, this is a naturally ripened staining solution with strong staining capacity and long storage stability. It is mainly used to demonstrate cross-striations in striated muscle. Clinically, this method is applied in the diagnosis of rhabdomyosarcoma, which shows diverse histological morphology and is difficult to distinguish from undifferentiated mesenchymal tumors. After phosphotungstic acid hematoxylin staining, the identification of blue cross-striations in the cytoplasm of tumor cells confirms rhabdomyoblastic differentiation. This staining solution can also stain fibrin in inflammatory exudates, fibrin in capillaries in DIC, and structures in neuropathology. This reagent is for research use only. Not for clinical diagnosis or other purposes.
Materials to be Prepared by the User:
1. 10% formalin fixative, acidified potassium permanganate solution, oxalic acid solution, distilled water, graded ethanol series
2. Xylene or eco-friendly dewaxing and clearing agent, neutral balsam
3. Light microscope
Operating Procedures (For Reference Only):
1. Fix tissue in 10% formalin fixative, followed by routine dehydration and embedding.
2. Prepare paraffin sections at 4 μm thickness. Dewax in xylene or dewaxing agent and hydrate to water using routine procedures.
3. Oxidize in acidified potassium permanganate for 5 minutes, then rinse briefly with water.
4. Bleach in oxalic acid solution for 1–2 minutes, wash in running tap water for 2 minutes, then rinse once with distilled water.
5. Stain in Mallory PTAH Staining Solution (Natural Oxidation Method) in a covered container for 24–48 hours.
6. Remove sections and quickly rinse excess stain with 95% ethanol.
7. Dehydrate routinely, clear in xylene or dewaxing agent, and mount with neutral balsam.
Staining Results:
Cross-striations of striated muscle, fibrin, nuclei, red blood cells, neuroglial fibers: Dark blue
Collagen fibers, cartilage matrix: Brownish red
Coarse elastic fibers: Purple
Precautions:
1. If the blue staining of cross-striations is insufficient or the striations appear bright red, this indicates insufficient or excessive oxidation. Replace the staining solution or prepare a new batch.
2. Do not wash sections with water after Mallory PTAH staining. Rinsing in 95% ethanol must be rapid, as prolonged washing or ethanol treatment will remove the stain.
3. Mallory Phosphotungstic Acid Hematoxylin is a progressive stain; avoid overstaining. After 24 hours of staining, sections may be removed and examined microscopically to assess staining intensity.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
Mallory三色染色试剂盒 | Mallory磷钨酸苏木素染色液
英文别名
Mallory PTAH Staining Solution (Natural Oxidation Method)
规格或纯度
BioReagent,生物染料级,用于显微镜
稳定性与储存
Store at room temperature long term (24 months). Store in the dark.
英文名称
Mallory PTAH Stain Solution (Spontaneous Oxidation Method)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333492 |
分析证书 | M1516033 |
技术文档和文章
生物染色剂
目录： 阿拉丁试剂的规格、级别和纯度
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
目录： 技术文章
Staining-Based Differentiation of Collagen Fibers, Muscle Fibers, and Fibrin in Tissue Sections
目录： 技术文章
Mallory Phosphotungstic Acid Hematoxylin Method for Staining Striated Muscle Cross-Striations, Fibrin, and Neuroglial Fibers: Experimental Protocol
目录： 实验方案（Protocols）
溶液计算器
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