L2522682计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
EnzymoPure™ 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。
概述
Characteristics of Micrococcal Nuclease from S. aureus:
Specificity
Exhibits both exo- and endo-5'-phosphodiesterase activities against both DNA and RNA (Alexander et al. 1961). The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate (Roberts et al. 1962; Rushisky et al. 1962).
Composition
The amino acid sequence has been reported by Cone et al. (1971). The active site has been reported on by Chaiken and Anfinsen (1971), Cuatrecasas (1970), and Cuatrecasas et al. (1968). See also: Furie et al. 1973), Bonhert and Taniuchi (1972), Sacks et al. (1972), Arnone et al. (1971), Epstein et al. (1971), Chaiken and Anfinsen (1970), Cohen et al. (1970), Markley et al. (1970), Putter et al. (1970), and Schechter et al. (1970).
Characteristics of Nuclease, Micrococcal
Molecular Weight
16,807 (Taniuchi et al. 1967b).
Optimal pH
The pH optimum is reported as 9.2. However, the enzymic activity has an absolute requirement for Ca2+ and the pH optimum varies according to Ca2+ concentration (Heins et al. 1967).
Extinction Coefficient
= 9.2 (Fuchs et al. 1967).
Inhibitors
5'-dioxynucleotides and 5'-ribonucleotides are inhibitory with the former demonstrating greatest inhibition.
Activity
Ca2+ is essential for activity. (See, however, Frank et al. 1975). Both RNase and DNase activities are competitively inhibited by deoxythymidine 3',5' diphosphate (Cuatrecasas et al. 1967). Sulkowski and Laskowski, Sr. (1970) indicate that the kinetics of degradation of RNA and DNA differ. They found no activation with Sr2+. See also: Dunn et al. (1973), Rammler et al. (1972), Chaiken and Anfinsen (1971), Markley and Jardetzy (1970) and Mikulski et al. (1969).
规格
产品名称
微球菌核酸酶 来源于金黄色葡萄球菌(菌株ATCC #27735)
规格或纯度
EnzymoPure™, ≥6,000 units/mg protein
生化机理
微球菌核酸酶对 DNA 的裂解作用在 A 或 T 核苷酸的 5′侧比在 G 或 C 侧更强。
CAS编号和信息
9013-53-0
酶学委员会编号
3.1.31.1
分子类型
酶
储存与运输
浓度
≥6,000 units/mg protein
储存条件
2-8°C储存
运输条件
低温运输
单位定义
Unit Definition: One Unit corresponds to a change in optical density of 1.0 at 260 nm at 37°C, pH 8.0, using DNA as the substrate.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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