BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
NADPH氧化酶（NAO）是一个典型的膜蛋白，催化NADPH氧化生成NADP⁺，并将电子传递给氧原子从而产生超氧阴离子。广泛存在于动物、植物和真菌中。该酶异常可导致人慢性肉芽肿病（GCD），在植物中，该酶与其抗病性和各种胁迫有密切关系。 NADPH氧化酶（NAO）将NADPH氧化为NADP⁺的同时生成超氧阴离子(O₂⁻ )，接着与显色剂反应生成水溶性的黄色物质。对照通过添加该酶的特异性抑制剂DPI排除背景值。最终检测生成的有色物质在450nm处的吸光值，即可计算得出NAO酶活性大小。
NADPH oxidase (NAO) is a typical membrane protein that catalyzes the oxidation of NADPH to NADP⁺ while transferring electrons to oxygen to generate superoxide anions. It is widely distributed in animals, plants, and fungi. Dysfunction of this enzyme can lead to chronic granulomatous disease (CGD) in humans, while in plants, it is closely associated with disease resistance and various stress responses. NADPH oxidase (NAO) converts NADPH to NADP⁺ and simultaneously generates superoxide anions (O₂⁻), which subsequently react with a chromogenic agent to form a water-soluble yellow product. Background values are excluded by adding the specific inhibitor diphenyleneiodonium (DPI) as a control. The activity of NAO is calculated by measuring the absorbance of the resulting colored product at 450 nm.
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