计算溶液所需的质量、体积或浓度。
镍柱原位清洁试剂盒
有货
为什么选择此级别
BioReagent,无菌过滤 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
是否无菌：是
镍柱介质是分离纯化His标记蛋白的重要材料，但反复使用后非常容易污染杂蛋白和杂质，是对His标记蛋白的回收率越来越低。为此本公司开发了镍柱原位清洁试剂盒，它具有下列特点:
1. 即开即用，不用单独准备各种溶液。
2. 原位清洁，直接在亲和层析柱中进行清洁，免去繁琐的装柱步骤。
3. 操作步骤简单，半小时即可完成。
4. 清洁的效果好，极大恢复镍柱介质的活性。
5. 可清除污染的离子型结合蛋白、沉淀蛋白、弱疏水性蛋白、脂蛋白、强疏水性蛋白和脂类。
6. 适用于各种镍柱介质(包括Ni-NTA或Ni-IDA)。
7. 本产品仅供科研使用。
产品组分
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使用方法
如果镍柱介质污染了其他蛋白和杂质，需要按下列步骤清洗；如果镍柱介质对His标记蛋白结合降低(蓝色越来越淡)，则需要另购镍柱原位再生试剂盒。
1. 去除镍柱中的离子型结合蛋白：用10倍介质体积的溶液A洗涤镍柱(对 1 mL的镍柱介质需要用10 mL溶液A洗涤)，再用10倍介质体积的自 备去离子水洗涤镍柱。处理后的镍柱介质根据需要可以直接使用，也可以保存在20%的乙醇溶液中于4℃长期保存，还可以继续下面的清洁步骤。
2. 去除镍柱中的沉淀蛋白、弱疏水性蛋白和脂蛋白：封堵住层析介质的下端出口，然后加入2倍介质体积的溶液B，再盖上上端口，颠倒混匀后浸泡2小时，再用10倍介质体积的溶液C洗涤镍柱介质，最后用10倍介质体积的自备去离子水洗涤镍柱。处理后的镍柱介质根据需要可以直接使 用，也可以保存在20%的乙醇溶液中于4℃长期保存，还可以继续下面的清洁步骤。
3. 去除镍柱中的强疏水性蛋白和脂类：封堵住层析介质的下端出口，然后加 入10倍介质体积的溶液D，再盖上上端口，颠倒混匀后浸泡20分钟， 再用10倍介质体积的自备去离子水洗涤镍柱。处理后的镍柱介质根据需要可以直接使用，也可以保存在20%的乙醇溶液中于4℃长期保存。
Nickel affinity resin is essential for purification of His-tagged recombinant proteins. However, repeated usage inevitably causes accumulation of miscellaneous proteins and contaminants, resulting in continuous decline of target protein recovery. To address this issue, we developed the In-situ Nickel Column Cleaning Kit with the following features:
1. Ready-to-use formulation; no extra buffer preparation required.
2. In-column cleaning: all treatments are performed directly in the affinity chromatography column without tedious resin repacking.
3. Straightforward procedures; the whole cleaning process can be finished within 30 minutes.
4. Excellent cleaning performance to effectively restore the binding activity of nickel resin.
5. Capable of removing ionic-bound proteins, precipitated proteins, weakly hydrophobic proteins, strongly hydrophobic proteins, lipoproteins and lipids.
6. Compatible with all types of nickel-charged resins including Ni-NTA and Ni-IDA.
7. For research use only.
Components
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Instruction for use
Proceed with the following cleaning steps if the nickel resin is contaminated by foreign proteins and impurities. Purchase the separate In-situ Nickel Column Regeneration Kit when the resin shows reduced binding toward His-tagged proteins accompanied by obvious fading of light-blue color.
1. Removal of ionically bound proteins: Wash the column with Solution A at 10 bed volumes (10 mL Solution A for 1 mL resin), then rinse with 10 bed volumes of user-provided deionized water. The treated resin can be used directly, stored long-term in 20% ethanol at 4 ℃, or subjected to subsequent cleaning steps as needed.
2. Removal of precipitated proteins, weakly hydrophobic proteins and lipoproteins: Seal the column bottom outlet, add Solution B at 2 bed volumes and cap the top inlet. Invert to mix thoroughly and incubate for 2 hours. Afterwards wash sequentially with 10 bed volumes of Solution C and 10 bed volumes of user-provided deionized water. The treated resin is available for immediate use, long-term storage in 20% ethanol at 4 ℃ or further cleaning.
3. Removal of strongly hydrophobic proteins and lipids: Block the column bottom outlet, add Solution D at 10 bed volumes and seal the top inlet. Invert for mixing and soak for 20 minutes, then rinse with 10 bed volumes of user-provided deionized water. The treated resin can either be used directly or preserved long-term in 20% ethanol at 4 ℃.
规格
别名
镍柱在位清洁试剂盒 | 镍琼脂糖层析柱清洁试剂盒
英文别名
Ni-Agarose Column in situ Cleaning Kit | Ni Column In-Place Cleaning Kit
规格或纯度
BioReagent, 无菌过滤
英文名称
Ni-Agarose Column In-Place Cleaning Kit
应用
Purification
储存与运输
储存条件
室温
运输条件
常规运输
稳定性与储存
各组分在相应的储存条件下保质期一年。
组分与储存
N1524123 | 组份 | 物理外观 | 10T | 20T | 储存 | 单次测试使用量 |
N1524123A | 溶液A | 无色透明液体 | 100 mL | 200 mL | 室温 | 10 mL |
N1524123B | 溶液B | 无色透明液体 | 20 mL | 40 mL | 室温 | 2 mL |
N1524123C | 溶液C | 无色透明液体 | 100 mL | 200 mL | 室温 | 10 mL |
N1524123D | 溶液D | 无色透明液体 | 100 mL | 200 mL | 室温 | 10 mL |
Note: The recommended amount of Ni resin for each test is 1 mL.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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