计算溶液所需的质量、体积或浓度。
| 活性类型 | 活性值-log(M) | 作用机制 | 期刊 | 参考文献(PubMed IDs) |
|---|
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 24 篇同行评审文献引用。
概述
Nocodazole is a reversible anti-neoplastic agent which exerts its effect in cells by interfering with the polymerization of microtubules (tubulin). Several drμgs including vincristine and colcemid are similar to nocodazole in that they interfere with microtubule polymerization. Nocodazole is frequently employed in cell biology laboratories to synchronize the cell division cycle; cells treated with nocodazole arrest with a G2- or M-phase DNA content when analyzed by flow cytometry. Nocodazole has also been used to induce the formation of Golgi ministacks in eukaryotic cells due to lack of proper Tubulin function.A potent Tubulin production inhibitor and anti-neoplastic agent
Information
Nocodazole (R17934, Oncodazole, NSC238159) is a rapidly-reversible inhibitor of microtubule polymerization, also inhibitsAbl,Abl(E255K)andAbl(T315I)withIC50of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole inducesapoptosis.
In vitro
Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. Nocodazole doubles HDR efficiency to up to 30% in iPSCs. It improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing.
In vivo
The tumor volume and tumor weight of the mice treated with Ketoconazole \u2009plus\u2009 Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole \u2009plus\u2009 Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole \u2009or\u2009 Nocodazole alone.
Cell Data
cell lines：
Concentrations：
Incubation Time：
Powder Purity:≥99%
规格
英文别名
Oncodazole, NSC238159 | methyl 6-(thiophene-2-carbonyl)-1H-benzo[d]imidazol-2-ylcarbamate
规格或纯度
10mM in DMSO
英文名称
Nocodazole (R17934)
生化机理
Nocodazole（R17934，Oncodazole，NSC238159）是一种快速可逆的微管聚合抑制剂，也能抑制 Abl、Abl(E255K) 和 Abl(T315I)，在无细胞实验中的 IC50 分别为 0.21 μM、0.53 μM 和 0.64 μM。Nocodazole 可诱导细胞凋亡。
储存条件
-80℃储存
运输条件
特低温运输
名称和识别符
EC号
250-626-5
分子类型
小分子
Isomeric SMILES
COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3
UN Number
Packing Group
I
分子量
301.32
Beilstein号
1085978
Reaxy-Rn
1018009
Reaxys-RN link address
https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1018009&ln=
技术文档
高级数据
关联靶点（人）
CYP1A2
Tchem
细胞色素 P450 1A2(Cytochrome P450 1A2)
(1 活性数据)
化学和物理性质
溶解性
Solubility (25°C) In vitro DMSO: 47 mg/mL (198.85 mM); Ethanol: 47 mg/mL (198.85 mM); Water: Insoluble;
熔点
260 °C
安全和危险性（GHS）
象形图
信号词
警告
危险声明
H227: 可燃液体
H341: 怀疑引起遗传缺陷
H361: 怀疑破坏生育力或未出生的孩子
预防措施声明
P201: 使用前获取特殊说明
P210: 远离热源，热表面，火花，明火和其他点火源。 - 禁止抽烟。
P202: 在阅读并理解所有安全预防措施之前，不要进行操作。
P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。
P308+P313: 如接触到或有疑虑：求医/就诊。
P370+P378: 火灾时：使用干砂、干粉或抗醇泡沫灭火。
P403: 存放在通风良好的地方。
P405: 密闭存放
P501: 将内容物/容器处理到。。。
WGK Germany
3
RTECS
DD6521000
Class
6.1
个人防护装备
Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 F2425005 |
分析证书 | N408505 |
技术文档和文章
Small-Molecule Inhibitors: A Practical Guide for Research Selection — A Minimal Evidence Chain and Scenario-Based Validation Framework (Including a Selection Matrix, Case Cards, and Product Tables 1–4)
目录： 技术文章
Mechanisms of Action, Research Applications, and Cold-Induced Effects of Colchicine: An Overview
目录： 技术文章
Furin: A Review of Its Structural Features, Substrate Recognition, and Research Applications
目录： 技术文章
细胞周期与DNA损伤应答机制研究
目录： 技术文章
此产品的引用文献
溶液计算器
常见问题
本产品应如何储存？
请于?80 °C条件下保存。需超低温保存以维持规定的有效期。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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危险品化学品经营许可证（带存储）