计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
中性红是一种弱碱性 pH 指示剂,变色范围在pH 6.4~8.0之间(由红变黄)。在中性或微碱性环境中,植物的活细胞能大量吸收中性红并向液泡中排泌。
中性红染色液 (1%) 是一种组织或细胞染色时常用的可以把细胞核染成红色的染色液,常用于组织或细胞染色中细胞核的红色复染。
使用方法
一、样品处理
1. 对于石蜡切片
(1) 二甲苯中脱蜡5~10 min。
(2) 换用新鲜的二甲苯,再脱蜡5~10 min。
(3) 无水乙醇5 min。
(4) 90% 乙醇2 min。
(5) 70% 乙醇2 min。
(6) 蒸馏水2 min。
2. 对于冰冻切片:恢复至室温后蒸馏水2 min。
3. 对于培养细胞
(1) 用4% 多聚甲醛固定10 min以上。
(2) 蒸馏水洗涤2 min。
(3) 换用新鲜的蒸馏水,再洗涤2 min。
二、中性红染色
1. 对于上述处理好的样品,用中性红染色液染色0.5~1 h。
2. 用蒸馏水或自来水充分洗涤。
3. 进行观察和拍照。
染色结果
| 细胞核 | 红色 |
注意事项
1. 如果需要脱水、透明和封片处理,还需自备二甲苯,中性树胶或其它封片剂。如果样品是石蜡切片,需自备70%和90% 乙醇,无水乙醇以及二甲苯。
2. 首次使用本试剂时建议先取1~2个样品做预实验。
3. 中性红染色时,可以根据染色结果和要求调整时间。
4. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
Neutral Red is a weakly basic pH indicator with a transition range of pH 6.4–8.0 (from red to yellow). In neutral or slightly alkaline environments, living plant cells can absorb a large amount of Neutral Red and secrete it into the vacuoles. Neutral Red Staining Solution (1%) is a commonly used reagent for tissue or cell staining, which stains the nucleus red. It is often used as a red nuclear counterstain in tissue or cell staining.
Protocol
I. Sample Preparation
1. For Paraffin Sections
(1) Deparaffinize in xylene for 5–10 min.
(2) Replace with fresh xylene and deparaffinize for another 5–10 min.
(3) Absolute ethanol for 5 min.
(4) 90% ethanol for 2 min.
(5) 70% ethanol for 2 min.
(6) Distilled water for 2 min.
2. For Frozen Sections
Equilibrate to room temperature, then rinse with distilled water for 2 min.
3. For Cultured Cells
(1) Fix with 4% paraformaldehyde for ≥10 min.
(2) Rinse with distilled water for 2 min.
(3) Replace with fresh distilled water and rinse for another 2 min.
II. Neutral Red Staining
1. Stain the prepared samples with Neutral Red Staining Solution for 0.5–1 h.
2. Wash thoroughly with distilled or tap water.
3. Observe and photograph.
Staining Result
| Structure | Color |
| Nuclei | Red |
Precautions
If dehydration, clearing and mounting are required, prepare xylene, neutral balsam or other mounting media by yourself. For paraffin sections, prepare 70% and 90% ethanol, absolute ethanol and xylene by yourself.
It is recommended to perform a pilot experiment on 1–2 samples when using this reagent for the first time.
The staining time with Neutral Red Staining Solution can be adjusted according to the staining results and requirements.
For your safety and health, please wear a lab coat and disposable gloves during operation.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | N774840 | |
| 分析证书 | N774840 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →