中性红是一种弱碱性pH指示剂,变色范围在 pH6.4~8.0之间 (由红变黄),分子式为 C₁₅H₁₆N₄・HCl,分子量为 288.78,在中性或微碱性环境中植物的活细胞能大量吸收中性红并向液泡中排泌,由于液泡在一般情况下呈酸性反应,因此进入液泡的中性红便解离出大量阳离子而呈现樱桃红色,在这种情况下原生质和细胞壁一般不着色。死细胞由于原生质变性凝固,细胞液不能维持在液泡内,用中性红染色后不产生液泡着色现象,相反中性红的阳离子,却与带有一定负电荷的原生质及细胞核结合,而使原生质与细胞核染色。
阿拉丁中性红染色液(1/3000)是一种组织或细胞染色时常用的可以把细胞核染成红色的染色液,常用于组织或细胞染色中细胞核的红色复染。该试剂仅用于科研领域,不适用于临床诊断或其他用途。由中性红、水等组成。
自备材料:
4%多聚甲醛、蒸馏水或PBS、系列乙醇
操作步骤(仅供参考):
1、细胞涂片用4%多聚甲醛固定10min以上。
2、中性红染色:Neutral Red Stain (1/3000) 染色2~5min。
3、用蒸馏水或PBS等充分洗涤。
4、进行观察和拍照。
注意事项:
1、首次使用本试剂时建议先取1~2个样品做预实验。
2、中性红染色液时,可以根据染色结果和要求调整时间。
3、试剂开封后请尽快使用,以防影响后续实验效果。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Neutral Red is a weakly basic pH indicator with a color change range of pH 6.4 to 8.0 (turning from red to yellow). Its molecular formula is C₁₅H₁₆N₄·HCl and molecular weight is 288.78. In a neutral or slightly alkaline environment, living plant cells can absorb a large amount of Neutral Red and secrete it into vacuoles. Since vacuoles generally show an acidic reaction, the Neutral Red entering the vacuoles dissociates a large number of cations and presents a cherry red color. Under such circumstances, the protoplast and cell wall are generally not stained. Due to the denaturation and coagulation of protoplast in dead cells, the cell sap cannot be maintained in the vacuoles, so no vacuole staining occurs after Neutral Red staining. On the contrary, the cations of Neutral Red combine with the protoplast and cell nucleus with a certain negative charge, thus staining the protoplast and cell nucleus.
Aladdin Neutral Red Staining Solution (1/3000) is a commonly used staining solution for tissue or cell staining that stains cell nuclei red, and is often used for the red counterstaining of cell nuclei in tissue or cell staining. This reagent is for research use only and not intended for clinical diagnosis or any other applications. Composed of Neutral Red, water, etc.
Materials to Be Prepared by the User:
4% paraformaldehyde, distilled water or PBS, graded ethanol series
Operating Procedures (For Reference Only):
1. Fix cell smears with 4% paraformaldehyde for more than 10 minutes.
2. Neutral Red staining: Stain with Neutral Red Stain (1/3000) for 2–5 minutes.
3. Rinse thoroughly with distilled water, PBS or other suitable solutions.
4. Perform observation and photography.
Precautions:
1. It is recommended to conduct a pre-experiment with 1–2 samples when using this reagent for the first time.
2. The staining time of Neutral Red Staining Solution can be adjusted according to staining results and requirements.
3. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
4. For your safety and health, wear a lab coat and disposable gloves during operation.