计算溶液所需的质量、体积或浓度。
免疫球蛋白G1 (IgG1), FC片段 来源于人骨髓瘤血浆
有货
为什么选择此级别
天然 级 提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
IgG Fc 段不具备抗原结合能力，但可调控抗体依赖性细胞毒性、补体依赖性细胞毒性与吞噬作用，通过 Fc 受体结合介导免疫功能。
蛋白生物学功能
免疫球蛋白 G 的 Fc（可结晶片段）区域无法结合抗原，但具备重要生物学活性与抗原决定簇。该同源二聚体糖蛋白由 CH2、CH3 结构域组成，通过与 Fc 受体、C1q 及 FcRn 相互作用介导免疫效应功能。Fc 片段调控抗体依赖性细胞毒性（ADCC）、补体依赖性细胞毒性（CDC）和吞噬作用；并依靠 pH 依赖性结合 FcRn，调控 IgG 血清半衰期。位于 Asn-297 的保守 N - 连接糖基化位点对维持结构稳定以及受体结合至关重要。
常见研究用途
Fc 糖基化模式改变与类风湿关节炎等自身免疫病相关；半乳糖基化、唾液酸化水平下降提示炎症状态。Fc 受体多态性同样与自身免疫病发病相关。常见应用：治疗药物研发、免疫系统研究、流式细胞检测实验。
While lacking antigen binding capacity, IgG Fc orchestrates antibody-dependent cytotoxicity, complement-dependent cytotoxicity, and phagocytosis. It mediates immune function through FC-receptor binding.
Role of protein in biology
The Fc (fragment crystallizable) region of immunoglobulin G, while not binding antigen, contains crucial biological activities and antigenic determinants. This homodimeric glycoprotein comprises CH2 and CH3 domains and mediates immune effector functions through interactions with Fc receptors, C1q, and FcRn.
Fc fragments orchestrate antibody-dependent cellular cytotoxicity, complement-dependent cytotoxicity, and phagocytosis while controlling IgG's serum half-life via pH-dependent FcRn binding. The conserved N-linked glycosylation at Asn-297 is essential for structural stability and receptor interactions.
Common research uses
Altered Fc glycosylation patterns correlate with autoimmune conditions including rheumatoid arthritis, where reduced galactosylation and sialylation indicate inflammatory disease states. Fc receptor polymorphisms are also associated with autoimmune disease development.
Common uses include therapeutics research, immune system research, flow cytometry assays.
规格
产品名称
免疫球蛋白G1 (IgG1), FC片段 来源于人骨髓瘤血浆
规格或纯度
天然, ≥95%(SDS-PAGE), 蛋白浓度见COA
生化机理
Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure. The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE.The constant region of Ig gamma-1 (IgG1) isotype interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions, including antibody-dependent cellular cytotoxicity or phagocytosis, complement-dependent cytotoxicity and inflammatory responses.
Post-translational: N-glycosylated. Carries predominantly biantennary complex-type glycans attached at Asn-180 residue on the Fc region of each heavy chain. Unique Fc glycan profiles found in secreted IgGs are induced in an antigen-specific way, likely programmed during B cell priming to mount an appropriate Ig effector response. The core glycan is composed of two sequential N-acetylglucosamine (GlcNAc) moieties followed by a central mannose (Man) from which two additional Man residues branch out (alpha1,3 and alpha1,6 antennae) each capped with a GlcNAc. Additional sugar molecules can be added to generate over 30 possible glycans. Such sugar modifications include the addition of one fucose at the initial GlcNAc, galactose (Gal) and sialic acid (Neu5Ac) residues at antennary GlcNAc or a bisecting GlcNAc to the core Man. Variable addition of sugars account for different IgG functional states associated with antibody-dependent cellular cytotoxicity or phagocytosis and inflammatory responses such as complement activation and cytokine secretion. Fc N-glycan diversity is further enhanced by asymmetric glycan pairing on the heavy chains. Fc N-glycan sialylation is linked to anti-inflammatory effects. It regulates Fc effector functions through conformational changes leading to preferential interaction with type II Fc receptors while reducing binding to type I Fc receptors. During plasmablast response, sialylated Fc domains within immune complexes signal via FCER2/CD23 and drive the selection of B cells with high affinity for antigen. Fc Igs carrying afucosylated N-glycans preferentially activate FCGR3A, antigen-dependent cellular cytotoxicity and antitumor immunity. (Microbial infection) Deglycosylation on Asn-180 by S. pyogenes EndoS or Endos2 endoglucosidases prevents interaction between immunoglobulin-gamma (IgG) and Fc receptors, impairing ability to activate the complement pathway.
种属
人(Human)
来源
天然
预测分子量
50 kDa
分子类型
蛋白质
产品线
biological_products
储存与运输
物理形态
液体
储存缓冲液
50 mM Tris-HCl, 200 mM NaCl, 0.05% NaN₃, pH 8.0.
防腐剂
0.05% Sodium azide
浓度
蛋白浓度见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃（>1年）；收货后建议分装，避免反复冻融。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
常见问题
本产品的纯度是多少？如何测定？
本产品的纯度为 ≥95%，采用SDS-PAGE测定。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）