计算溶液所需的质量、体积或浓度。
磷钨酸负染色液（5%）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
负染色又称阴性染色，是由Hall发现的相对于普通染色（即正染色）而言的染色技术。其原理在于利用重金属盐包绕低电子密度的样品，增强样本四周的电子密度，造成细微结构之间的质量－厚度差异，增强散射吸收反差，使样品在黑暗的背景上呈现明亮的结构。负染色液有磷钨酸、钼酸铵、印度墨汁等，其中最常用的是1~3%磷钨酸。
磷钨酸负染色液（5%）适用于显示大分子、细菌、病毒、原生动物、噬菌体、细胞器、核酸大分子、蛋白质晶体及其他大分子材料等。染色后的样品图像呈现透明的亮光，而背景图像呈黑色。
使用说明
一、滴染法
1. 样品低速离心 (2000g，10min) 或采用其他方法浓缩样品，制成悬浮液并且使其达到一定浓度和纯度。
2. 将样品悬浮液直接滴于带有支持膜的载网上，静置3~5min。
3. 用滤纸条从液滴边缘吸去多余液体，稍干燥。
4. 滴加负染色液，静置2~3min。
5. 吸去多余染色液，自然干燥，进行显微镜观察。
二、漂浮法
1. 样品低速离心 (2000g，10min) 或采用其他方法浓缩样品，制成悬浮液并且使其达到一定浓度和纯度。
2. 将带有支持膜的载网置于样品液滴上漂浮以沾取样品。
3. 载网置于负染色液上漂浮1~2min。
4. 吸去多余染色液，自然干燥，进行显微镜观察。
注意事项
1、目的样本尽量新鲜。
2、样品应为均匀的悬浮液，其纯度和浓度应适宜，否则无法与染色剂之间产生特异和清晰的结合反应。
3、对于大多数样品的负染，2～3%磷钨酸已经足够，5%磷钨酸可用于较难染色的样本。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
染色结果
| 样品 | 透明的亮光 |
| 背景 | 黑色 |
Negative staining, also known as negative contrast staining, is a staining technique discovered by Hall, as opposed to conventional staining (i.e., positive staining). Its principle lies in using heavy metal salts to surround low-electron-density samples, enhancing the electron density around the specimen and creating differences in mass-thickness between fine structures. This enhances the scattering absorption contrast, making the sample appear as bright structures against a dark background. Negative staining solutions include phosphotungstic acid, ammonium molybdate, India ink, etc., with 1–3% phosphotungstic acid being the most commonly used.
Phosphotungstic Acid Negative Staining Solution (5%) is suitable for visualizing macromolecules, bacteria, viruses, protozoa, bacteriophages, organelles, nucleic acid macromolecules, protein crystals, and other macromolecular materials. After staining, the sample appears as transparent bright structures, while the background appears black.
Instructions for Use
1. Droplet Staining Method
- Concentrate the sample by low-speed centrifugation (2000g, 10 min) or other methods to prepare a suspension with appropriate concentration and purity.
- Directly drop the sample suspension onto a grid with a support film and let it stand for 3–5 minutes.
- Use filter paper strips to absorb excess liquid from the edge of the droplet and allow it to dry slightly.
- Add a drop of negative staining solution and let it stand for 2–3 minutes.
- Absorb the excess staining solution, allow it to air-dry naturally, and proceed with microscopic observation.
2. Floating Method
- Concentrate the sample by low-speed centrifugation (2000g, 10 min) or other methods to prepare a suspension with appropriate concentration and purity.
- Float the grid with a support film on a droplet of the sample suspension to pick up the sample.
- Float the grid on the negative staining solution for 1–2 minutes.
- Absorb the excess staining solution, allow it to air-dry naturally, and proceed with microscopic observation.
Precautions
The target sample should be as fresh as possible.
The sample should be a homogeneous suspension with appropriate purity and concentration; otherwise, a specific and clear binding reaction with the staining agent may not occur.
For most sample negative staining, 2–3% phosphotungstic acid is sufficient, while 5% phosphotungstic acid can be used for samples that are more difficult to stain.
For your safety and health, please wear a lab coat and disposable gloves during operation.
Staining Results
| Sample | Transparent bright structures |
| Background | Black |
规格
别名
阴性染色 | 负染色
英文别名
Hosphotungstic Acid Stain
规格或纯度
BioReagent, 生物染色剂, 用于显微镜, 5%
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Hosphotungstic Acid Negative Staining Solution (5%)
储存条件
避光,室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131055 |
分析证书 | P1508549 |
技术文档和文章
溶液计算器
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