计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
纤维素是由葡萄糖构成的大分子多糖,为植物细胞壁的主要成分,也是自然界分布最广、含量最高的多糖,占植物界碳含量 50% 以上。棉花的纤维素含量接近 100%,是天然最纯的纤维素来源;一般木材中纤维素占 40%~50%,同时含有 10%~30% 的半纤维素和 20%~30% 的木质素。纤维素常与半纤维素、果胶、木质素结合存在,其结合方式与程度对植物源食品的质地影响显著,而植物成熟与后熟过程中的质地变化主要由果胶物质改变引起。人体消化道不含纤维素酶,纤维素是一类重要的膳食纤维。
植物纤维素染色液(氯碘化锌法)基于氯碘化锌显色反应,可使纤维素呈现紫蓝色或蓝色,木质素、角质及软木脂呈橙黄色,适用于新鲜组织切片,以及经乙醇、FAA 固定液固定或冰冻干燥处理的组织切片染色。本品仅用于科研领域,不适用于临床诊断或其他非科研用途。
自备材料
操作步骤(仅供参考)
1. 切片置于载玻片上,立即用入植物纤维素染色液(氯碘化锌法)浸染。
2. 染色5 min后即可置于显微镜下观察,染色时间越长,颜色越深。
染色结果
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注意事项
1. 组织固定常采用乙醇、FAA固定液,避免使用铬酸固定剂。
2. 如果没有切片机可以采用徒手切片法,但需挑选比较薄且均匀的组织切片。
3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
4. 试剂开封后请尽快使用,以防影响后续实验效果。
Cellulose is a macromolecular polysaccharide composed of glucose, serving as the primary component of plant cell walls. It is also the most widely distributed and abundant polysaccharide in nature, accounting for over 50% of the carbon content in the plant kingdom. Cotton fiber contains nearly 100% cellulose, making it the purest natural source of cellulose. In general wood, cellulose constitutes 40%–50%, along with 10%–30% hemicellulose and 20%–30% lignin. Cellulose often coexists with hemicellulose, pectin, and lignin. The manner and degree of their association significantly affect the texture of plant-based foods, while texture changes during plant maturation and post-harvest ripening are primarily caused by alterations in pectic substances. The human digestive tract lacks cellulase, making cellulose an important type of dietary fiber.
Plant Cellulose Staining Solution (Zinc Chloroiodide Method) is based on the colorimetric reaction of chlorine-zinc iodide. It stains cellulose violet-blue or blue, while lignin, cutin, and suberin appear orange-yellow. It is suitable for staining fresh tissue sections, as well as tissue sections fixed with ethanol or FAA fixative, or processed by freeze-drying. This product is intended for research use only and is not suitable for clinical diagnosis or other non-research purposes.
Materials to Be Prepared by User
Procedure (For Reference Only)
1. Place the tissue section on a glass slide and immediately immerse it in the Plant Cellulose Staining Solution (Chlorine-Zinc Iodide Method) for staining.
2. After staining for 5 minutes, the section can be observed under a microscope. Longer staining times result in deeper colors.
Staining Results
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Notes
For tissue fixation, ethanol or FAA fixative is commonly used. Avoid using chromic acid fixatives.
If a microtome is unavailable, free-hand sectioning may be used, but thin and uniform tissue sections should be selected.
For your safety and health, please wear a lab coat and disposable gloves during operation.
Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | P1511470 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →