细胞或组织用福尔马林固定后，会导致蛋白之间、蛋白与核酸之间的交联，从而遮蔽抗原表位，影响免疫组化的染色结果。选用合适的抗原修复液，采用热修复方法，可打开上述交联，充分暴露抗原表位，达到抗原修复的目的，同时本品中含有的脱蜡试剂成分可将切片中所含石蜡从组织中分离，从而在抗原热修复的过程中同时完成脱蜡和水化过程。
石蜡切片脱蜡抗原修复液，又称一步法脱蜡/抗原修复液、脱蜡热修复液、脱蜡修复液，由Tris、EDTA、脱蜡试剂、PC300及去离子水配制而成，使用时需用去离子水稀释20倍，并调整pH至6.0。本产品摆脱传统石蜡切片处理对实验的限制和束缚，无需通风橱和传统的脱蜡缸，将传统的2-3次二甲苯脱蜡、3-5次梯度乙醇水化和抗原修复整合成一个溶液，有效的缩短了操作时间，简化了繁琐的操作步骤，同时，减少了操作中的变量，提高了后期染色的稳定性。本产品采用新的环保技术，不仅把对身体的危害降到了最低，而且对环境不会造成任何污染。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、系列乙醇、双蒸水或去离子水、免疫染色洗涤液
2、加热设备 (微波炉、水浴锅、高压锅等)
3、经福尔马林固定、石蜡包埋 (FFPE)的组织切片
操作步骤 (仅供参考)：
1、配制脱蜡抗原修复工作液：石蜡切片脱蜡抗原修复液 (20×, 低pH)为20倍浓缩液，pH呈酸性，实验前用19倍去离子水稀释成脱蜡抗原修复工作液。例如：取25 ml石蜡切片脱蜡抗原修复液 (20×, 低pH)，加入去离子水定容至500 ml，调整pH至6.0即可。
2、脱蜡水化及抗原修复：
(1) 将脱蜡抗原修复工作液放入修复缸中，使用加热设备加热至沸腾。
(2) 将切片放入沸腾的脱蜡抗原修复工作液中 (为保证修复液的pH值，不能使用金属切片架)，保证液体完全浸没切片上的组织。
(3) 中小火持续加热15-30 min，此过程中勿使组织干片，将修复缸撤离加热源，自然冷却至室温。
(4) 取出切片，用去离子水冲洗切片，确保没有脱蜡抗原修复液的残留 (此过程中切勿对着组织冲洗，以免弄破组织)。
(5) 参考IHC实验操作步骤进行后续实验。
注意事项：
1、切片浸泡在脱蜡抗原修复工作液中的最佳时间需根据不同的样品自行摸索。
2、实验人员必须经专业培训，使用前应仔细阅读本说明书。
3、本产品仅用于免疫组化的脱蜡及抗原修复，不做其他用途。
4、本说明书所述脱蜡、抗原修复方式为通常情况所用方式，用户可根据具体切片性质和抗原类型进行调整或预实验，脱蜡或抗原修复不充分可能造成染色不均匀或强度不足。
5、过度修复可能造成组织破坏或脱落。
6、加热过程完成后应自然降温，否则会影响抗原修复效果。
7、为了您的安全和健康，请穿实验服并戴一次性手套操作。
8、试剂开封后请尽快使用，以防影响后续实验效果。
Formalin fixation of cells or tissues causes cross-linking between proteins and between proteins and nucleic acids, which masks antigenic epitopes and impairs the results of immunohistochemical staining. Selecting a suitable antigen retrieval solution and adopting a heat-induced retrieval method can break these cross-links and fully expose antigenic epitopes to achieve the purpose of antigen retrieval. Meanwhile, the deparaffinization reagent components contained in this product can separate paraffin from tissues in sections, thus completing the deparaffinization and hydration processes simultaneously during heat-induced antigen retrieval.
Paraffin Section Deparaffinization Antigen Retrieval Solution, also known as One-step Deparaffinization/Antigen Retrieval Solution, Deparaffinization Heat Retrieval Solution and Deparaffinization Retrieval Solution, is prepared from Tris, EDTA, deparaffinization reagents, PC300 and deionized water. It should be diluted 20-fold with deionized water and adjusted to pH 6.0 before use. This product breaks the limitations of traditional paraffin section processing on experiments and eliminates the need for fume hoods and traditional deparaffinization jars. It integrates the traditional procedures of 2-3 rounds of xylene deparaffinization, 3-5 rounds of gradient ethanol hydration and antigen retrieval into a single solution, effectively shortening the operation time and simplifying tedious steps. At the same time, it reduces variables in the operation and improves the stability of subsequent staining. Adopting a new environmentally friendly technology, this product not only minimizes harm to the human body but also causes no environmental pollution. This reagent is for research use only and not intended for clinical diagnosis or any other applications.
Materials to Be Prepared by the User
1. Graded ethanol, double-distilled water or deionized water, immunostaining wash buffer
2. Heating equipment (microwave oven, water bath, autoclave, etc.)
3. Formalin-fixed, paraffin-embedded (FFPE) tissue sections
Operating Procedures (For Reference Only)
1. Prepare Deparaffinization Antigen Retrieval Working Solution: Paraffin Section Deparaffinization Antigen Retrieval Solution (20×, Low pH) is a 20-fold concentrated solution with alkaline pH. Dilute it 19-fold with deionized water to prepare the working solution before the experiment. Example: Take 25 ml of Paraffin Section Deparaffinization Antigen Retrieval Solution (20×, Low pH) and add deionized water to make the final volume up to 500 ml, and adjust the pH to 6.0.
2. Deparaffinization, Hydration and Antigen Retrieval:
(1) Place the deparaffinization antigen retrieval working solution in a retrieval jar and heat it to boiling using heating equipment.
(2) Immerse the sections in the boiling working solution (metal slide racks are not allowed to ensure the pH of the retrieval solution) and ensure the tissue on the sections is completely submerged.
(3) Continue heating on low to medium heat for 15-30 minutes. Do not let the tissue dry out during this process. Remove the retrieval jar from the heat source and allow it to cool to room temperature naturally.
(4) Take out the sections and rinse them with deionized water to ensure no residual deparaffinization antigen retrieval solution is left (Do not rinse directly at the tissue to avoid tissue damage).
(5) Perform subsequent experiments with reference to the standard IHC experimental procedures.
Precautions
1. The optimal immersion time of sections in the deparaffinization antigen retrieval working solution needs to be optimized by the user according to different samples.
2. Operators must receive professional training and read this instruction manual carefully before use.
3. This product is exclusively for deparaffinization and antigen retrieval in immunohistochemistry and no other uses are permitted.
4. The deparaffinization and antigen retrieval methods described in this manual are standard protocols. Users may adjust the protocols or perform pre-experiments according to the specific properties of sections and types of antigens. Insufficient deparaffinization or antigen retrieval may lead to uneven staining or reduced staining intensity.
5. Over-retrieval may cause tissue damage or detachment.
6. The solution must be cooled naturally after heating; otherwise, the antigen retrieval effect will be impaired.
7. For your safety and health, wear a lab coat and disposable gloves during operation.
8. Use the reagent as soon as possible after opening to avoid compromising the results of subsequent experiments.
ZJ26F0433944
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