BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
天然果胶类物质以原果胶、果胶(Pectin)、果胶酸的形态广泛存在于植物的果实、根、茎、叶中，是细胞壁的一种组成成分，它们伴随纤维素而存在，构成相邻细胞中间层粘结物，使植物组织细胞紧紧黏结在一起。果胶酶(Pectinase)是一类分解果胶质酶类的总称，实质是多聚半乳糖醛酸水解酶，包括原果胶酶，果胶酯酶，多聚半乳糖醛酸酶和果胶裂解酶四大类，广泛存在于植物果实和微生物中，主要用于食品、酿酒、环保、医药、纺织及日化用品行业。 该试剂盒检测原理是果胶酶水解果胶生成β-半乳糖醛酸，与二硝基水杨酸(DNS)反应形成有色化合物，该化合物呈色强度与半乳糖醛酸浓度成正比，于分光光度计540nm处测定吸光度，通过与标准曲线比较计算出样品中果胶酶活性，主要用于定量检测植物组织或果实中果胶酶，反应颜色越深，吸光度越大，果胶酶的活性越强。本产品仅用于科研领域，不适用于临床诊断或其他用途。
Natural pectin substances exist widely in the fruits, roots, stems, and leaves of plants in the forms of protopectin, pectin, and pectic acid. They are components of the cell wall, accompanying cellulose, and constitute the intercellular adhesive layer between adjacent cells, firmly bonding plant tissue cells together. Pectinase is a general term for a class of enzymes that decompose pectin substances. Essentially, they are polygalacturonate hydrolases, including four main types: protopectinase, pectinesterase, polygalacturonase, and pectin lyase. They are widely found in plant fruits and microorganisms and are primarily used in the food, brewing, environmental protection, pharmaceutical, textile, and daily chemical industries. The detection principle of this kit is as follows: Pectinase hydrolyzes pectin to produce β-galacturonic acid, which reacts with dinitrosalicylic acid (DNS) to form a colored compound. The color intensity of this compound is proportional to the concentration of galacturonic acid. The absorbance is measured at 540 nm using a spectrophotometer. By comparing with a standard curve, the pectinase activity in the sample is calculated. This kit is primarily used for the quantitative determination of pectinase activity in plant tissues or fruits. The darker the reaction color and the higher the absorbance, the stronger the pectinase activity. This product is for research use only and is not intended for use in diagnostic procedures.
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