本产品是一种新型无血清快速细胞冻存液，能够有效提高细胞冻存活率和复苏活力，不含血清，可减少各类病毒、细菌、霉菌和支原体等污染，保证冻存细胞的安全。该产品既适用于一般培养细胞的冻存，也适用于无血清培养细胞的冻存。
产品特点
1、省时省力——直接存放于-80℃冰箱冻存，无需程序降温；
2、安全性高——无血清，化学成分明确；
3、高品质——细胞复苏率在90%以上；
4、使用方便——2~8℃即可稳定保存，无需冷冻，无需分装，即取即用。
已验证细胞
目前已验证的细胞：HEP3B，SNU-387，SNU-423，HLE，JHH-7，A375，A2058，A431，HEK-293，Hela，A549，NCI-H460，CHO，MCF-7，HepG2，C2C12，COS7， DU145，MDCK，NIH-3T3，MSC，PC12，RAW264.7，769-P，HK-2，T24，M14，U87，U251，293FT，Jurkat，Raji，K562，HCCLM3，Vero，MC3T3-E1，HCT116，H9C2，SiHa，SK-OV-3等。
使用说明
细胞冻存步骤
1、收集处于对数生长期贴壁细胞或悬浮细胞于离心管中，并进行细胞计数；
2、取所需冻存细胞数的细胞悬浮液置于离心管中，离心收集细胞(参考离心条件：1,000~2,000rpm，3~5min)。彻底移去离心管中的上清液；
3、加入适量的细胞冻存液于离心管中，调整细胞浓度至1~5×10⁶cells/mL。轻柔混匀，制成细胞冻存悬液；
4、将离心管中的细胞冻存悬液分装于已标示完全的冻存管中；
5、直接将含细胞悬液的冻存管放入-80℃冰箱冷冻保存；
6、若需液氮保存，需放-80℃冰箱过夜后移至液氮罐。
细胞复苏步骤
1、取出冻存的细胞，立即放入37℃水浴槽中快速解冻；
2、待冻存管中细胞混合液完全融化后，立即移入含5mL细胞培养基的离心管中，离心收集细胞(参考离心条件：1,000~2,000rpm，3~5min)；
3、小心移去上清液。加入适量的新鲜细胞培养基，轻轻混匀，将细胞混合液移至事先准备好的培养容器中；
4、镜检后，可根据具体研究需要及方法进行细胞常规培养。
注意事项
1、本产品长期反复开盖使用可能会有微量晶体状沉淀出现，此为正常现象，不影响细胞冻存效果。建议分装使用，即可避免此情况发生，同时也可减小操作过程中交叉污染的风险；
2、冻存细胞分装后，应尽快移入-80℃冰箱；
3、若需长期冻存细胞(≥6个月)，请转移至液氮罐中贮存；
4、冻存干细胞或原代细胞时，建议事先使用该产品对所冻存的细胞进行为期至少1周的预实验，确认性能后再进行正式冻存；
5、冻存液中含有DMSO成分，为了您的安全和健康，请穿实验服并戴一次性手套操作；
6、本产品仅限科研使用。
This product is an advanced serum-free cell cryopreservation solution designed to significantly improve cell survival and recovery rates after freezing. Without containing any serum, it reduces the risk of contamination by viruses, bacteria, fungi, and mycoplasma, ensuring the safety of preserved cells. It is suitable for cryopreserving both conventionally cultured cells and serum-free adapted cell lines.
Features
Time-Saving & Convenient: Cells can be directly stored in a -80°C freezer without the need for programmed cooling.
High Safety: Serum-free and chemically defined composition.
High Quality: Ensures post-thaw cell viability exceeding 90%.
Ease of Use: Stable at 2–8°C; ready to use without thawing.
Validated Cell Lines
The following cell types have been successfully preserved with this product:
HEP3B, SNU-387, SNU-423, HLE, JHH-7, A375, A2058, A431, HEK-293, HeLa, A549, NCI-H460, CHO, MCF-7, HepG2, C2C12, COS7, DU145, MDCK, NIH-3T3, MSC, PC12, RAW264.7, 769-P, HK-2, T24, M14, U87, U251, 293FT, Jurkat, Raji, K562, HCCLM3, Vero, MC3T3-E1, HCT116, H9C2, SiHa, SK-OV-3, among others.
Instructions for Use
Cell Freezing Procedure
Harvest adherent or suspension cells in the logarithmic growth phase and perform cell counting.
Transfer the cell suspension into a centrifuge tube and pellet the cells by centrifugation (recommended: 1,000–2,000 rpm, 3–5 min). Aspirate the supernatant completely.
Add an appropriate amount of cryopreservation medium into the tube and adjust the cell concentration to 1–5×10⁶ cells/mL. Mix gently to form a homogeneous suspension.
Aliquot the cell suspension into clearly labeled cryovials.
Place the cryovials directly into a -80°C freezer for storage.
For long-term storage in liquid nitrogen, transfer cryovials to a liquid nitrogen tank after overnight storage at -80°C.
Cell Thawing Procedure
Quickly thaw frozen cryovials in a 37°C water bath with gentle agitation.
Once fully thawed, immediately transfer the contents to a centrifuge tube containing 5 mL of cell culture medium. Centrifuge (1,000–2,000 rpm, 3–5 min) to pellet the cells.
Carefully remove the supernatant. Resuspend the cells in an appropriate volume of fresh culture medium and transfer to a prepared culture vessel.
After microscopic examination, proceed with routine cell culture as required.
Precautions
Repeated opening and use of this product may cause minor crystalline precipitation, which is normal and does not affect performance. Aliquot usage is recommended to avoid this and minimize cross-contamination.
After aliquoting cells, transfer cryovials to the -80°C freezer as soon as possible.
For long-term storage (≥6 months), transfer cryovials to a liquid nitrogen tank.
When cryopreserving stem cells or primary cells, conduct a preliminary test using this product for at least one week to verify performance.
This product contains DMSO. Wear a lab coat and disposable gloves during use.
For research use only.
ZJ26F0738091
危险品化学品经营许可证（带存储）