L1811136计算溶液所需的质量、体积或浓度。
| 活性类型 | 活性值-log(M) | 作用机制 | 期刊 | 参考文献(PubMed IDs) |
|---|
为什么选择此级别
提供 ≥95%(UV) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 3 篇同行评审文献引用。
概述
7-Ethoxyresorufin is a fluorimetric substrate and suicide inhibitor of CYP (cytochrome P450). Also shown to inhibit several reductase enzymes, specifically CYPOR (NADPH-P450 reductase). The binding to CYP (cytochrome P450) is thoμght to inhibit the vasorelaxant response to acetylcholine. 7-Ethoxyresorufin has also demonstrated inhibition of NO synthesis by uncoupling neuronal NOS1 (nitric oxide synthase); it is a noncompetitive inhibitor of neuronal NOS1 with respect to L-arginine with a Ki value of 0.76 +/- 0.06 μM. 7-Ethoxyresorufin is a substate of CYP1A1.7-Ethoxyresorufin (7-ER) is a substrate used in environmental toxicology studies to monitor ethoxyresorufin-O-deethylase catalytic activity in the EROD assay. The EROD assay monitors the induction of the xenobiotic-metabolizing enzyme cytochrome P-450 (CYP) 1A1 and is a widely used biomarker for exposure of wildlife to substances that bind the aryl hydrocarbon (Ah) receptor. It provides evidence of receptor-mediated induction of cytochrome P450-dependant monooxygenases (the CYP1A subfamily specifically) by xenobiotic chemicals.
7-Ethoxyresorufin is a fluorimetric substrate and suicide inhibitor of CYP (cytochrome P450). Also shown to inhibit several reductase enzymes, specifically CYPOR (NADPH-P450 reductase). The binding to CYP (cytochrome P450) is thought to inhibit the vasorelaxant response to acetylcholine. 7-Ethoxyresorufin has also demonstrated inhibition of NO synthesis by uncoupling neuronal NOS1 (nitric oxide synthase); it is a noncompetitive inhibitor of neuronal NOS1 with respect to L-arginine with a Ki value of 0.76 +/- 0.06 μM. 7-Ethoxyresorufin is a substate of CYP1A1.
7-Ethoxyresorufin (7-ER) is a substrate used in environmental toxicology studies to monitor ethoxyresorufin-O-deethylase catalytic activity in the EROD assay. The EROD assay monitors the induction of the xenobiotic-metabolizing enzyme cytochrome P-450 (CYP) 1A1 and is a widely used biomarker for exposure of wildlife to substances that bind the aryl hydrocarbon (Ah) receptor. It provides evidence of receptor-mediated induction of cytochrome P450-dependant monooxygenases (the CYP1A subfamily specifically) by xenobiotic chemicals.
规格
别名
7-乙氧基resorufin
英文别名
7-ER | 7-Ethoxy-3H-phenoxazin-3-one | SCHEMBL104497 | FT-0668125 | UNII-59AF853SW7 | BDBM21360 | J-100239 | 59AF853SW7 | HY-D0145 | AS-59402 | CHEBI:34480 | RESORUFINETHYLETHER | Q27116097 | Resorufinethyl ether | 7-Ethoxyresorufin | 3H-Phenoxazin-3-one,
规格或纯度
≥95%(UV)
英文名称
Resorufin ethyl ether
生化机理
荧光微粒体脱烷基酶和CYP450底物。产生红色荧光染料试卤灵（Ex / Em 571/585 nm）。适用于xA0区分CYP同工酶1A1和1A2。
应用
7-Ethoxyresorufin (7-ER) is a substrate used in environmental toxicology studies to monitor ethoxyresorufin-O-deethylase catalytic activity in the EROD assay. The EROD assay monitors the induction of the xenobiotic-metabolizing enzyme cytochrome P-450 (CYP) 1A1 and is a widely used biomarker for exposure of wildlife to substances that bind the aryl hydrocarbon (Ah) receptor. It provides evidence of receptor-mediated induction of cytochrome P450-dependant monooxygenases (the CYP1A subfamily specifically) by xenobiotic chemicals.
储存条件
-20°C储存
运输条件
超低温运输
备注
有关更多信息，请参阅SDS。有关溶解度，用法和处理的更多建议？请访问我们的常见问题（FAQ）页面以获取更多详细信息。
纯度
≥95%(UV)
名称和识别符
PubChem SID
分子类型
小分子
IUPAC Name
7-ethoxyphenoxazin-3-one
INCHI
1S/C14H11NO3/c1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h3-8H,2H2,1H3
InChi Key
CRCWUBLTFGOMDD-UHFFFAOYSA-N
Smiles
CCOC1=CC2=C(C=C1)N=C3C=CC(=O)C=C3O2
Isomeric SMILES
CCOC1=CC2=C(C=C1)N=C3C=CC(=O)C=C3O2
PubChem CID
分子量
241.24
Beilstein号
225973
技术文档
高级数据
系统学分类
分类树（Taxonomy Tree）
| 界（kingdom) | 有机化合物 |
|---|---|
| 超类（Superclass) | 有机杂环化合物 |
| 类（Class) | 苯并噁嗪类 |
| 亚类（Subclass） | 吩噁嗪类 |
| 中间层级节点（Intermediate Tree Nodes） | 暂无 |
| 直接上位类(Direct Parent) | 吩噁嗪类 |
| 其他上位类（Alternative Parents） | 烷基芳基醚 苯类化合物 杂芳族化合物 环酮 氧环化合物 氮杂环化合物 有机光子化合物 有机氮化合物 有机氧化物 碳氢化合物衍生物 |
| 分子骨架（Molecular Framework) | 芳香族杂多环化合物 |
| 取代基（Substituents） | 芳基醚 - 芳香族杂多环化合物 - 氮杂环 - 苯基化合物 - 环酮 - 乙醚 - 杂芳香族化合物 - 烃衍生物 - 有机硝基化合物 - 有机氧化物 - 有机氧化合物 - 有机氮化合物 - 有机氧化合物 - 有机氮化合物 - 氧杂环 - 苯氧嗪 |
| 描述（Description） | 该化合物属于苯并噁嗪类有机化合物。这类多环芳香化合物含有苯并噁嗪结构单元，该单元为由两个苯环经1,4-噁嗪环连接而成的线性三环体系。 |
| 外部描述符（External Descriptors） | phenoxazine |
三维结构
交互式化学结构模型
关联靶点（人）
CYP1A2
Tchem
细胞色素 P450 1A2(Cytochrome P450 1A2)
(2 活性数据)
APP
Tclin
淀粉样β蛋白A4(Amyloid-beta A4 protein)
(1 活性数据)
APP
Tclin
Amyloid-beta A4 protein
(8510 活性数据)
CYP1A2
Tchem
Cytochrome P450 1A2
(26471 活性数据)
作用机制
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
化学和物理性质
溶解性
Soluble in DMSO (5 mg/mL), ethanol (5 mg/mL), chloroform, dichloromethane, DMF (5 mg/mL), alcohols, and methanol. Insoluble in water.
分子量
241.240 g/mol
XLogP3
1.800
氢键供体数Hydrogen Bond Donor Count
0
氢键受体数Hydrogen Bond Acceptor Count
4
可旋转键计数Rotatable Bond Count
2
精确质量Exact Mass
241.074 Da
单同位素质量Monoisotopic Mass
241.074 Da
拓扑极表面积Topological Polar Surface Area
47.900 Ų
重原子数Heavy Atom Count
18
形式电荷Formal Charge
0
复杂度Complexity
450.000
同位素原子数Isotope Atom Count
0
定义的原子立体中心计数Defined Atom Stereocenter Count
0
未定义的原子立体中心计数Undefined Atom Stereocenter Count
0
定义的键立体中心计数Defined Bond Stereocenter Count
0
未定义的键立体中心计数Undefined Bond Stereocenter Count
0
所有立体化学键的总数The total count of all stereochemical bonds
0
共价键合单元计数Covalently-Bonded Unit Count
1
质检证书（CoA,COO,BSE/TSE 和分析图谱)
此产品的引用文献
引用文献
| 1. Wei Liu, Mi Zhang, Yan Xiao, Zhaoyang Ye, Yan Zhou, Meidong Lang, Wen-Song Tan. (2020) Fabrication and in vitro evaluation of a packed-bed bioreactor based on galactosylated poly(ethylene terephthalate) microfibrous scaffolds. BIOCHEMICAL ENGINEERING JOURNAL, [10.1016/j.bej.2020.107565] |
| 2. Tongtong Shen, Yufan Miao, Chenchen Ding, Wentao Fan, Shuhui Liu, Yanan Lv, Xiaona Gao, Marthe De Boevre, Liping Yan, Sheila Okoth, Sarah De Saeger, Suquan Song. (2019) Activation of the p38/MAPK pathway regulates autophagy in response to the CYPOR-dependent oxidative stress induced by zearalenone in porcine intestinal epithelial cells. FOOD AND CHEMICAL TOXICOLOGY, [PMID:31173817] [10.1016/j.fct.2019.05.035] |
| 3. Yingchang Song, Jiayu Zeng, Jianglan Long, Aiting Wang, Kuan Chen, Jia'an Qin, Dan Yan. (2024) Rapid multichannel fluorescent probe assay for CYP450 inhibition screening and drug interaction monitoring. MICROCHEMICAL JOURNAL, [10.1016/j.microc.2024.110185] |
溶液计算器
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