计算溶液所需的质量、体积或浓度。
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在色谱分析、有机合成和交叉偶联反应领域已被 2 篇同行评审文献引用。
D-Lactate Dehydrogenase (LDHD) is an enzyme that catalyzes the oxidation of D-lactate to pyruvate, and it belongs to the lactate dehydrogenase family.
Application: This enzyme can be used for the enzymatic determination of various metabolites, such as ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol, as well as the enzyme activity when coupled with related enzymes including GPT, PK and CPK.
Procedure
Principle

Unit Definition
Reagents
A. Sample diluent: Add 1 g BSA and 1.36 g potassium dihydrogen phosphate to 800 mL deionized water, adjust pH to 7.0 with sodium hydroxide (25℃), and adjust to 1 L. (Stable at 2-8℃ for 2 months)
B. Reagent 1: Add 12.1 g Tris to 800 mL deionized water, adjust pH to 7.70 with hydrochloric acid (25℃), then add 1 g sodium azide and stir until dissolved. Finally, make up to 1 L with water. To prepare, add an appropriate volume of sodium pyruvate to 0.4g/L of the solution. (Store at 2-8℃. Keep for one week.)
C. Reagent 2: Dissolve 21.1 grams of CAPS (3-cyclohexylamino propionium sulfate) in 800 mL deionized water, adjust pH to 9.70 with sodium hydroxide, then add 1.20 grams of NADH and 1 gram of sodium azide. Stir until fully dissolved and adjust to 1 liter. (Store at 2-8℃; shelf life: 1 week)
D. Sample to be tested: Before testing, weigh the required enzyme in a Weighing tube. When weighing the enzyme, at least 10mg of enzyme should be weighed to ensure the accuracy of weighing. Dissolve the enzyme in the dilution solution and adjust the volume to the preparation volume.
Procedure
Calculation

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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | R1506878 | |
| 分析证书 | R1506878 | |
| 分析证书 | R1506878 | |
| 分析证书 | R1506878 |
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