Name: 酰基辅酶A合成酶 (ACS)
SKU: R1506902
Availability: InStock

酰基辅酶A合成酶 (ACS)

CAS: 9013-18-7 货号: R1506902 EC号: 232-747-5

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,生物活性,重组,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

Acyl-CoA synthetase catalyzes the activation reaction of fatty acids. In the presence of ATP, CoA and Mg²⁺, it can catalyze the activation of fatty acids to form acyl-CoA.

Application: Suitable for the enzymatic determination of free fatty acid content. Each laboratory shall determine the optimal working titer for its specific application. Other applications have not been tested, but their use in such assays should not be excluded.

Assay Procedure

Principle

Unit Definition

One unit of enzyme activity unit is equal to the amount of enzyme required to convert 1 micromole of palmitic acid in 1 minute under the following test conditions.

Reagents

Reagent A: Prepare a 0.05M potassium phosphate buffer solution with pH 7.5.

Reagent B: Add 0.6053 g of ATP·Na₂, 0.2033 g of hexahydrate magnesium chloride, and 0.8216 g of Coenzyme A to 0.8 L of Reagent A, adjust pH to 7.5, and adjust to 1 L with Reagent A. (Preparation is optional; Coenzyme A is unstable and can be added as needed.)

Reagent C: Add 50 g Triton X-100 and 2 mmol palmitic acid to 0.8 L of water. Heat to dissolve the palmitic acid completely, then cool to room temperature. Adjust pH to 7.5 with sodium hydroxide and adjust to 1 L with Reagent A (preparation by proportion).

Reagent D: Add 0.1251 g NEM (maleimide), 0.505 g 4-aminotetraphenylpyrimidine, 8kU peroxidase, and 0.25 g sodium azide to 0.8 L of Reagent A, adjust pH to 7.5, and adjust to 1 L with Reagent A (preparation by proportion).

Reagent E: Add 2 grams of phenol to 80ml of Reagent A and adjust the volume to 100ml with Reagent A (preparation by proportion).

Reagent F: ACS Dilution Buffer: Add 2 mM ATP and 1 g Triton X-100 to 1 L of 10 mM potassium phosphate buffer at pH 7.5. (Preparation can be scaled up proportionally).

Reagent G: ACOD Dilution Buffer: Add 0.02 mmol FAD and 0.2 g bovine serum albumin to 1 L of 25 mM potassium phosphate buffer at pH 7.5. (Preparation by proportion is possible).

Reagent H: Prepare a 240 kU/L ACOD (acetyl-CoA oxidase) enzyme solution using Reagent G.

Test sample: Weigh the required enzyme in a Weighing tube before testing. When weighing the enzyme, at least 10 mg of enzyme should be weighed to ensure accuracy. Dissolve the enzyme in diluent (Reagent F) and adjust to the specified volume.

Procedure

1. Mix 320μl of Reagent B with 80μl of Reagent C and incubate at 37℃ for 1 minute.

2. Add 20μl of diluted ACS enzyme solution and incubate at 37℃ for 10 minutes.

3. Add 800μl of Reagent D and 20μl of Reagent E, incubate at 37℃ for 2 minutes; then add 20μl of Reagent H and incubate at 37℃ for 5 minutes, recording the absorbance value (As).

4. Repeat steps 1-3, but replace the ACS enzyme solution with 20 μL of ACS dilution. Record the blank absorbance value (Ab).

Note: △A=As-Ab≤0.300; the optimal linear range for this method is 100-200 U/L.

Calculation

The enzyme activity is calculated using the following formula:

Vt: Total volume of the reaction mixture (1.26ml)

ε: The generated quinuimine exhibits an extinction coefficient at 500 nm of 12 (cm²/μmol)

Vs: Volume of the enzyme solution to be tested (ml)

d: Light path length of the cuvette (cm)

D: Dilution factor of the enzyme solution

△A/min: As-Ab

规格

产品名称

酰基辅酶A合成酶 (ACS)

英文别名

long-chain-fatty-acid--CoA ligase | acyl-activating enzyme | acyl-CoA synthetase | fatty acid thiokinase (long chain) | lignoceroyl-CoA synthase | FAA1 | FAA4 | FAA3 | FAA2 | FAM1

规格或纯度

生物活性,重组,ActiBioPure™,高性能,EnzymoPure™,≥1 U/mg enzyme powder

生物活性

≥1 U/mg enzyme powder

表达系统

E. coli

来源

重组表达

CAS编号和信息

9013-18-7

酶学委员会编号

EC 6.2.1.3

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥1 U/mg enzyme powder

复溶

Reconstitute in pure water.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃（24个月）；复溶后建议分装，避免反复冻融。

单位定义

One unit of enzyme activity unit is equal to the amount of enzyme required to convert 1 micromole of palmitic acid in 1 minute under the following test conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ26F0434113	分析证书	R1506902
ZJ26F0434112	分析证书	R1506902
ZJ26F0434111	分析证书	R1506902

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目录：技术文章

探索主题:

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酰基辅酶A合成酶 (ACS)

储存与运输

质量文档

文献证明

概述

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

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