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Recombinant Human Clusterin Protein (rp144473) - Protein Bioactivity
Measured by its ability to induce clustering of Caki‑2 human clear cell carcinoma epithelial cells. The effective concentration is 10μg/mL.
ActiBioPure™,His标签,无动物源,无叠氮钠,无载体,生物活性,高性能 级 提供 ≥90%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Purity
>90% SDS-PAGE.
Function
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
Post-translational
Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Polyubiquitinated, leading to proteasomal degradation. Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
Recombinant Human Clusterin Protein (rp144473) - Protein Bioactivity
Measured by its ability to induce clustering of Caki‑2 human clear cell carcinoma epithelial cells. The effective concentration is 10μg/mL.
Recombinant Human Clusterin Protein (rp144473) - SDS-PAGE
3 μg/lane of Recombinant Human Clusterin Protein (rp144473) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 36.2 kDa under reducing conditions and 60.7 kDa under non-reducing conditions.
Recombinant Human Clusterin Protein (rp144473) - ELISA
Immobilized Recombinant Human Clusterin Protein (rp144473) at 1.0 μg/mL can bind AB-16B5 (anti-Clusterin) (Ab177846) with the EC50 of 33.58 ng/mL.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp144473 | |
| 分析证书 | rp144473 | |
| 分析证书 | rp144473 |
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