1、Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells.Recombinant Human Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.2-0.8 µg/mL. 2、Measured by its binding ability in a functional ELISA. When Recombinant Human Osteopontin (rp149597) is immobilized at 4 µg/mL, biotinylated Recombinant Human Mesothelin (rp176301) binds with an ED50 of 40.2 ng/mL.
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Post-translational
Extensively phosphorylated on clustered serine residues. N- and O-glycosylated. Phosphorylation sites are present in the extracelllular medium.
规格
产品名称
Recombinant Human Osteopontin Protein
别名
重组人 Osteopontin 蛋白 | 重组人骨桥蛋白
英文别名
BNSP | Bone sialoprotein 1 | Bone sialoprotein I | BSP I | BSPI | Early T lymphocyte activation 1 | ETA 1 | ETA1 | MGC110940 | Nephropontin | OPN | Osteopontin | osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein | OSTP_HUMAN |
1、Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells.Recombinant Human Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.2-0.8 µg/mL. 2、Measured by its binding ability in a functional ELISA. When Recombinant Human Osteopontin (rp149597) is immobilized at 4 µg/mL, biotinylated Recombinant Human Mesothelin (rp176301) binds with an ED50 of 40.2 ng/mL.
Reconstitute in water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可存放 1 年以上
图片
Recombinant Human Osteopontin Protein (rp149597) - Protein Bioactivity Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Recombinant Human Osteopontin Protein (rp149597) coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37℃. The ED₅₀ for this effect is 0.2 - 0.8 µg/mL.
Recombinant Human Osteopontin Protein (rp149597) - Protein Bioactivity Measured by its binding ability in a functional ELISA. When Recombinant Human Osteopontin (rp149597) is immobilized at 4 µg/mL, biotinylated Recombinant Human Mesothelin (rp176301) binds with an ED₅₀ of 40.2 ng/mL.
Recombinant Human Osteopontin Protein (rp149597) - SDS-PAGE 3 μg/lane of Recombinant Human Osteopontin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 59.7 & 17.0 & 13.1 kDa.
ZJ24F0505686
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