Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.
Purity >98% SDS-PAGE. Function Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
规格
产品名称
Recombinant Human RANK Protein
别名
重组人 RANK蛋白 | 核因子-κB 受体激活因子 | 重组人类 RANK 蛋白
英文别名
CD 265 | CD265 | FEO | LOH18CR1 | Loss of heterozygosity 18 chromosomal region 1 | mRANK | ODFR | OFE | OPTB7 | Osteoclast differentiation factor receptor | OSTS | Paget disease of bone 2 | PDB 2 | PDB2 | RANK | Receptor activator of NF KB | Receptor acti
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.
20.5 kDa, under reducing conditions; 18.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
应用
Cell Culture,Active,SDS-PAGE
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 2-8 ℃ 下保存一个月。分装后在 -80 ℃ 保存 12 个月。避免冷冻/解冻循环。
图片
Recombinant Human RANK protein (rp150851) - SDS-PAGE Recombinant Human RANK protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 20.5 kDa under reducing conditions and 18.2 kDa under non-reducing conditions.
Recombinant Human RANK protein (rp150851) - Protein Bioactivity Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit RANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/mL, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg in the presence of 15 ng/mL of recombinant RANK Ligand.
ZJ23F0700625
危险品化学品经营许可证（带存储）