级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。高性能?高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc标签?Fc 标签级 —— 带有 Fc 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc 标签有助于二聚化和 protein A/G 捕获。≥95%(SDS-PAGE)
Accession #
Q9Y6Q6
蛋白标签
C-His & hFc
表达系统
HEK293
内毒素水平
<0.1 EU/μg
生物活性
Measured by its ability to inhibit TRANCE-induced osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 0.005‑0.035 µg/mL in the presence of 2 ng/mL of rmTRANCE.
Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2. Involved in the regulation of interactions between T-cells and dendritic cells.
生物活性
Measured by its ability to inhibit TRANCE-induced osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 0.005‑0.035 µg/mL in the presence of 2 ng/mL of rmTRANCE.
Recombinant Human RANK/TNFRSF11A Protein (rp150852) - SDS-PAGE
2 μg/lane of Recombinant Human RANK/TNFRSF11A Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 57 kDa under reducing conditions and 118 & 250 kDa under non-reducing conditions.
The stability of the RANK protein's extracellular domain is conferred by intramolecular disulfide bonds formed by its cysteine residues. Prior to ligand binding, the Pre-Ligand Binding Assembly Domain (PLAD) mediates constitutive dimerization, a prerequisite for forming the active RANK-RANKL trimeric complex. This complex assembly triggers downstream signaling essential for bone metabolism and immune regulation, a well-defined mechanism in structural biology.
ZJ25F1029142
危险品化学品经营许可证（带存储）