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Recombinant Rat GM-CSF Protein (rp155067)-Protein Bioactivity
Measured in a cell proliferation assay using FDC-P1 cells. The ED₅₀ for this effect is typically <0.01ng/mL.
ActiBioPure™,PBS Only,无动物源,无叠氮钠,无载体,生物活性,高性能 级 提供 ≥98%(SDS-PAGE&HPLC) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
GM-CSF是一种造血生长因子,刺激中性粒细胞和巨噬细胞的发育,促进早期红系巨核细胞和嗜酸性祖细胞的增殖和发育。它由内皮细胞、单核细胞、成纤维细胞和t淋巴细胞产生。GM-CSF抑制中性粒细胞迁移,增强成熟端细胞的功能活性。GM-CSF也被报道对非造血细胞有功能性作用,并可诱导人内皮细胞迁移和增殖。此外,它还能刺激多种肿瘤细胞系的增殖,包括成骨肉瘤、癌和腺癌细胞系。据报道,GM-CSF没有跨物种的生物学效应。重组鼠GM-CSF是一个14.5kDa的球状蛋白,由127个氨基酸残基组成。
Purity
>98% SDS-PAGE. > 98 % by HPLC.
Additional sequence information
This product is for the mature full length protein. The signal peptide is not included.
Function
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Background
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. It is reported that GM-CSF has no biological effects across species. Recombinant Rat GM-CSF is a 14.5kDa globular protein consisting of 127 amino acid residues.
Recombinant Rat GM-CSF Protein (rp155067)-Protein Bioactivity
Measured in a cell proliferation assay using FDC-P1 cells. The ED₅₀ for this effect is typically <0.01ng/mL.
Recombinant Rat GM-CSF Protein (rp155067)-SDS-PAGE
3μg/lane of Recombinant Rat GM-CSF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.7 kDa.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp155067 | |
| 分析证书 | rp155067 | |
| 分析证书 | rp155067 | |
| 分析证书 | rp155067 |
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