Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description: Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), which is a member of orthopoxvirus genus. A29L is an intracellular mature virus (IMV) surface envelope protein that is homologous to vaccinia virus (VACV) A27 and is widely conserved in the Poxviridae family. The A27L protein (p14) is a multifunctional protein that has been implicated in virus attachment, virus–cell fusion, cell–cell fusion, plaque size, and the formation of enveloped virions. The involvement of A27 in mature virus (IMV) inhibits the assembly of fusion protein A26. After MVs are endocytosed by vesicles, the acidic endocytic environment activates the dissociation of A26 protein from MVs, resulting in fusion of the viral membrane with the vesicle membrane. A27 interacts with the integral membrane protein A17, and then forms a protein complex with A26 through disulfide bond linkage to anchor the A26 protein to mature virus (MV) particles.
13.2 kDa, under reducing conditions; 37-46 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
biological_products
储存与运输
应用
WB,ELISA
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, PH7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C stable for 1 year, store at 2-8℃ for 1 month. Avoid freeze / thaw cycle.
图片
Recombinant MPXV A29L Protein (rp155836) - SDS-PAGE 3 μg/lane of Recombinant MPXV A29L Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a band at 13.2 kDa under reducing conditions and 37-46 kDa under non-reducing conditions.