Em:517nm,Ex:498nm,无叠氮钠 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光,禁止冷冻。低温运输,禁止冷冻 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
背景
链霉亲和素（Streptavidin）是由streptomyces avidinii菌分泌的四聚体蛋白，可以高度特异性地和生物素（Biotin）结合，结合常数为10⁻¹⁴ mol/L，一分子 Streptavidin 可以结合 4 个 Biotin 分子，这种结合是目前自然界已知最强的非共价作用。 Streptavidin 和鸡蛋清来源的亲和素（Avidin）相比，两者与 Biotin 结合的亲和力高度相似性， 但Streptavidin在中性条件下几乎不带电荷，因此Streptavidin的非特异性结合比Avidin低很多， 检测时的非特异性背景也就少很多。Streptavidin 可以用于 Biotin 标记的抗体、核酸、蛋白或其他生物素标记分子的检测。Streptavidin (FITC)产品是用高纯度的 Streptavidin 和高纯度的 FITC 染料共价连接，并去除游离FITC。FITC标记的链霉亲和素是间接法荧光染色中一种非常有用的二抗，可以高亲和性地结合生物素化抗体。FITC由488nm的激光激发，可以在最大波长为520nm处检测到其发射光。
推荐工作浓度
Flow cytometry: 1:500-1:5000
IF: 1:50-1:1000
Background
Streptavidin is a tetrameric bacterial protein isolated from Streptomyces avidinii providing 4 high-affinity biotin binding sites. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin. With a dissociation constant on the order of ≈10⁻¹⁴ mol/L, the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Unlike egg-white avidin, which has a net positive charge at neutral pH and contains about 7% carbohydrate, streptavidin has almost no net charge at neutral pH, does not contain carbohydrate, and exhibits lower non-specific background. Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules. This FITC-streptavidin conjugate was prepared by highly purified Streptavidin and free FITC was removed. Streptavidin (FITC) is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. Excitation at 488nm light leads to a fluorescence emission maximum of 520 nm.
Recommended Usage：
Every lot of Streptavidin-FITC is tested by flow cytometry using biotinylated primary antibodies. From this testing it is recommended that between 0.02 and 0.25 µg of streptavidin be used per 106 cells in a 100 µl staining volume.
ICC/IF-Streptavidin protein (FITC) (rp155876)
ICC/IF with Streptavidin protein (FITC) (rp155876) stained HeLa cells. The cells were methanol fixed (5 min) and blocked with 1% BSA . The cells were then incubated with Biotin-γ-actin antibody overnight at 4°C. Then Streptavidin protein (FITC) rp155876 (green) was used at 1:1000 for 1h. DAPI was used to stain the cell nuclei (blue) .
ICC/IF-Streptavidin protein (FITC) (rp155876)
ICC/IF with Streptavidin protein (FITC) (rp155876) stained HeLa cells. The cells were methanol fixed (5 min) and blocked with 1% BSA . The cells were then incubated with Biotin-γ-actin antibody overnight at 4°C. Then Streptavidin protein (FITC) rp155876 (green) was used at 1:1000 for 1h. DAPI was used to stain the cell nuclei (blue) .
Flow-Streptavidin protein (FITC) (rp155876)
BALB/c splenocytes stained with Biotin-CD45R antibody, followed by Streptavidin protein (FITC) (rp155876) at 1/2500 dilution.
Streptavidin Protein (FITC) (rp155876) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Streptavidin Protein (FITC) (rp155876) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
1:500-1:5000
1:50-1:500
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0319537 |
分析证书 | rp155876 |
| ZJ24F1216185 |
分析证书 | rp155876 |
| ZJ24F1216186 |
分析证书 | rp155876 |
| ZJ23F1202116 |
分析证书 | rp155876 |
| ZJ23F1202117 |
分析证书 | rp155876 |
| ZJ23F1202118 |
分析证书 | rp155876 |
| ZJ23F0300089 |
分析证书 | rp155876 |
| ZJ23F0300090 |
分析证书 | rp155876 |
| ZJ23F0300091 |
分析证书 | rp155876 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Azide Free 级别指南 → 查看 Ex:498nm 级别指南 → 查看 Em:517nm 级别指南 →
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