Recombinant Human IL-6 Protein

货号: rp156005
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 无叠氮钠 ? 无叠氮(Azide Free)—— 不含叠氮化钠防腐剂。适用于叠氮有毒或有抑制作用的偶联、细胞工作或检测。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 PBS Only ? 仅 PBS 配方 —— 以磷酸盐缓冲液供应,不含其他添加剂。当需要不含稳定剂/载体的洁净 PBS 缓冲液时使用。 ≥95%(SDS-PAGE)
Accession #
P05231
蛋白标签
N-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.123 ng/mL. Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 μg/mL can bind MEDI-5117 (anti-IL-6) (Ab182698) with the EC50 of 13.84 ng/mL.
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规格
库存
价格
数量
10μg
rp156005-10μg
现货 Stock Image
¥799.90
50μg
rp156005-50μg
现货 Stock Image
¥1,999.90
100μg
rp156005-100μg
现货 Stock Image
¥3,599.90
1mg
rp156005-1mg
期货 Stock Image
¥13,999.90
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为什么选择此级别

ActiBioPure™,His标签,PBS Only,无叠氮钠,无载体,生物活性,高性能 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining
Description:
Interleukin-6 (IL-6) is a pleiotropic, alpha-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand-binding subunit (IL-6 R alpha) and a signal-transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. Gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

规格

产品名称
Recombinant Human IL-6 Protein
别名
重组人白细胞介素-6蛋白 | 重组人IL-6蛋白 | 重组人 IL-6 蛋白
英文别名
Interleukin BSF 2 | B cell differentiation factor | B cell stimulatory factor 2 | B-cell stimulatory factor 2 | BSF 2 | BSF-2 | BSF2 | CDF | CTL differentiation factor | Cytotoxic T cell differentiation factor | Hepatocyte stimulatory factor | Hepatocyte
规格或纯度
无载体,生物活性,ActiBioPure™,无叠氮钠,高性能,His标签,PBS Only,≥95%(SDS-PAGE)
生物活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.123 ng/mL. Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 μg/mL can bind MEDI-5117 (anti-IL-6) (Ab182698) with the EC50 of 13.84 ng/mL.
表达系统
E. coli
种属
人(Human)
氨基酸
30-212 aa
序列
MGSSHHHHHHSSGLVPRGSHMVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM
纯度
>95% (SDS-PAGE)
蛋白标签
N-His
无载体
Yes
无动物源
No
来源
重组表达
预测分子量
23.1 kDa
SDS-PAGE
24.1 kDa, under reducing condition
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
图片

Recombinant Human IL-6 Protein (rp156005) - Protein Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.123 ng/mL.

Recombinant Human IL-6 Protein (rp156005) - SDS-PAGE
3 μg/lane of Recombinant Human IL-6 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 24.1 kDa.

Recombinant Human IL-6 Protein (rp156005) - ELISA
Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 μg/mL can bind MEDI-5117 (anti-IL-6) (Ab182698) with the EC ₅₀   of 13.84 ng/mL.

技术文档

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高级数据

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找到4个结果

批号(Lot Number) 证书类型 货号
ZJ24F0606476 分析证书 rp156005
ZJ24F1216139 分析证书 rp156005
ZJ24F1216140 分析证书 rp156005
ZJ24F0606477 分析证书 rp156005
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Regulatory Significance of the EPO-ERFE-Hepcidin Axis in Iron Utilization and Erythropoiesis
Aladdin® Target Proteins
单宁酸-透钙磷石原位复合骨水泥的制备策略与体外生物性能评价
Preparation Strategy and In Vitro Biological Performance Evaluation of Tannic Acid–Brushite In Situ Composite Bone Cement
代谢综合征标志物怎么联合分析:胰岛素抵抗、脂肪因子网络与慢性炎症检测策略
How to Jointly Analyze Metabolic Syndrome Biomarkers: Detection Strategies for Insulin Resistance, Adipokine Networks, and Chronic Inflammation
一文读懂干细胞分类和应用
Understanding Stem Cell Classification and Applications
溶液计算器

常见问题

本产品的纯度是多少?如何测定?
本产品的纯度为 ≥95%,采用SDS-PAGE测定。批次的具体数值以分析证书(COA)为准。
什么是「无载体」?
「无载体」表示该物料经过加工和检测,适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如,电子应用的金属含量、电池应用的粒度、光学应用的折射率,或对水分敏感工艺的水分含量。
什么是「生物活性」?
「生物活性」表示该产品经过加工和检测,适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性,以及在相关情况下的生物活性验证。
本产品应如何储存?
请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。
本产品如何运输?
本产品采用保温箱配冰袋运输。收货后请立即拆包,并按上述储存条件保存。
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