| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp156465-1KU (试用装) 申请此免费试用装（?） 作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！
|
1KU |
现货  |
| |
| rp156465-10KU |
10KU |
现货 |
|
| 产品名称 | Recombinant EN-TEV Protease Protein |
|---|---|
| 别名 | 重组EN-TEV Protease蛋白 | 重组 EN-TEV 蛋白酶蛋白 |
| 英文别名 | NIa | P1 Protease | TEV Protease | Tobacco Etch Virus Protease |
| 规格或纯度 | 无载体, 生物活性, ActiBioPure™, 无叠氮钠, ≥98%(SDS-PAGE), 见COA |
| 产品介绍 |
TEV Protease is the 241 amino acid (aa), 27 kDa catalytic domain of the nuclear inclusion a (NIa) protein encoded by the potyvirus, tobacco etch virus (TEV). It may be used in biotechnology to cleave affinity tags from recombinant proteins, either co-translationally orin vitrofollowing purification. Its high specificity and activity at a wide range of pH and ionic strength make TEV Protease more versatile than many other proteases used for the same purpose. Unlike factor Xa, enteropeptidase or thrombin, TEV Protease has not been found to cleave at unintended sites, even when present at a high concentration. TEV Protease is a 3C-type protease that cleaves substrates with a consensus sequence of ENLYFQG. Cleavage occurs between Q and G. Since the final aa remains on the cleaved protein where it could potentially affect structure or function, substitution of a variety of aa have been tested. In order of efficiency, S, A, M, Y, D, N, E, K or L may be effectively used in place of G. Several of the remaining aa may also vary, giving a final consensus sequence of ExxYF(M)Q(E)/G(S, A or others) where aa in parenthesis are alternatives and x is any aa. The autocatalytic site of NIa at S2256 has been mutated to an N for improved stability of the protease. |
| 生物活性 | Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser , with the cleavage point after Gln. One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.Complete digestion of 50µg of fusion KGF-MBP tag protein using 1µg TEV enzyme (in 1/50 ratio) in either 1 hours at 30 °C or overnight at 4 °C. The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT. |
| 表达系统 | E. coli |
| 种属 | Tobacco etch virus (TEV) |
| 氨基酸 | 2038-2279 aa |
| 序列 | GHHHHHHHGESLFKGPRDYNPISSSICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLVVQSLHGVFKVKDTTTLQQHLVDERDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSSTFPSGDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFANTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMNRRRRR |
| 纯度 | ＞98% (SDS-PAGE) |
| 蛋白标签 | N-His |
| 无载体 | Yes |
| Accession # | P04517 |
| 来源 | 重组表达 |
| 预测分子量 | 28.6 kDa |
| SDS-PAGE | 28.6 kDa |
Recombinant EN-TEV Protease protein (rp156465) - SDS-PAGE
3 μg/lane of Recombinant EN-TEV Protease protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 28.6 kDa.
| 物理形态 | 液体 |
|---|---|
| 储存缓冲液 | 25mM HEPES buffer, 400mM NaCl, PH7.5 |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。 |
| 分子类型 | 蛋白质 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ23F0800865 |
分析证书 | 23-08-22 | rp156465 |
| ZJ23F0800864 |
分析证书 | 23-08-22 | rp156465 |
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