Measured in a cell proliferation assay using human MCF 7 cells. The ED50 for this effect is < 5 ng/mL. The corresponding specific activity is>2×10^5 IU/mg.
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM.
生物活性
Measured in a cell proliferation assay using human MCF 7 cells. The ED50 for this effect is < 5 ng/mL. The corresponding specific activity is>2×10^5 IU/mg.
Reconstitute in water to a concentration of 1 mg/mL, for other concentrations, please adjust the concentration of phosphate in the solution by yourself.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存 -20℃~-80℃（60个月）；复溶后建议分装，避免反复冻融。
图片
Recombinant Human LR3 IGF-I/IGF-1 Protein (rp166305) - SDS-PAGE 2 μg/lane of Recombinant Human LR3 IGF-I/IGF-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 10 kDa.
Recombinant Human LR3 IGF-I/IGF-1 Protein (rp166305) - Protein Bioactivity Measured in a cell proliferation assay using human MCF7 cells. The ED50 for this effect is < 5 ng/mL. The corresponding specific activity is>2×10^5 IU/mg.
ZJ25F0723791
危险品化学品经营许可证（带存储）