The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
生物活性
Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <4.6 ng/mL.
表达系统
毕赤酵母
种属
人(Human)
氨基酸
49-118 aa
序列
GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA
纯度
>95% SDS-PAGE
蛋白标签
No tag
N-末端序列
Gly
蛋白长度
Full length protein
无载体
Yes
无动物源
Yes
来源
重组表达
预测分子量
7.7 kDa
SDS-PAGE
8.5 kDa, under reducing conditions; 8.5 kDa, under non-reducing conditions.
CAS编号和信息
67763-96-6
分子类型
蛋白质
产品线
生物产品
储存与运输
应用
Petrochemicals,Refining
物理形态
冻干(Lyophilized)
储存缓冲液
10mM磷酸钠缓冲液
复溶
Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
Recombinant Human IGF-1 Protein (rp147252)-Protein Bioactivity Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <4.6ng/mL.
Recombinant Human IGF-1 Protein (rp147252)-SDS-PAGE 5μg/lane of Recombinant Human IGF-1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 8.5 kDa.