1、Measured by its ability to inhibit the biological activity of IGF-I on MCF‑7 human breast cancer cells. The ED50 for this effect is 73.91 ng/mL in the presence of 2 ng/mL Recombinant Human IGF-1 Protein (rp147252). 2、Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-5 Protein (rp167866) at 1 μg/mL can bind Human IGF-1 Protein, the ED50 for this effect is 88.81 ng/mL.
Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells. Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells. Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling. Also plays a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs. Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling.
Post-translational: Cleaved by C1S in extracellular space.
生物活性
1、Measured by its ability to inhibit the biological activity of IGF-I on MCF‑7 human breast cancer cells. The ED50 for this effect is 73.91 ng/mL in the presence of 2 ng/mL Recombinant Human IGF-1 Protein (rp147252). 2、Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-5 Protein (rp167866) at 1 μg/mL can bind Human IGF-1 Protein, the ED50 for this effect is 88.81 ng/mL.
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20~-80℃（1年）；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IGFBP-5 Protein (rp167866) - SDS-PAGE 2 μg/lane of Recombinant Human IGFBP-5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 30 - 37 kDa.
Recombinant Human IGFBP-5 Protein (rp167866) - Protein Bioactivity Measured by its ability to inhibit the biological activity of IGF-I on MCF‑7 human breast cancer cells. The ED50 for this effect is 73.91 ng/mL in the presence of 2 ng/mL Recombinant Human IGF-1 Protein (rp147252).
Recombinant Human IGFBP-5 Protein (rp167866) - Protein Bioactivity Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-5 Protein (rp167866) at 1 μg/mL can bind Human IGF-1 Protein, the ED50 for this effect is 88.81 ng/mL.
ZJ25F0521437
危险品化学品经营许可证（带存储）