Measured by its binding ability in a functional ELISA. Immobilized human NSE (rp169606) at 1.0 μg/mL can bind NSE Mouse mAb (Ab118776) with the ED50 of 4.75 ng/mL.
Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Neuron specific enolase (NSE), also known as ENO2 or gamma-enolase, is a dimeric, Mg2+-dependent enzyme that catalyzes the dehydration of 2-phospho-D glycate (PGA) to phosphoenolpyruvate (PEP) in the glycolytic pathway and catalyzes the reverse reaction in gluconeogenesis. There are three major isozymes of enolase expressed in selective vertebrate tissues from separate genes: alpha (ENO1), beta (ENO3), and gamma (ENO2). NSE is a highly expressed, specific neuron isozyme making it a useful marker for tumors derived from neuronal cells. Neuron-specific enolase is implicated as a diagnostic and prognostic marker in numerous diseases including early small cell lung cancer, prostate cancer, multiple myeloma, traumatic brain injury, acute spinal cord injury, acute ischemic stroke, and post-concussion symptoms. NSE expression and activity are increased in neuronal and glial activation and injury, risk factors implicated in neurodegenerative disease. Elevation of NSE promotes glycolysis, proliferation, activation and migration through its C-terminus to activate PI3K and MAPK signal transduction pathways while inhibition of enolase has been shown to attenuate inflammatory events. NSE can be regulated through cleavage of the C-termini by cathepsin X or inhibited directly by antibiotic SF2312. Inhibition has been proposed as a therapeutic strategy in cancer.
Measured by its binding ability in a functional ELISA. Immobilized human NSE (rp169606) at 1.0 μg/mL can bind NSE Mouse mAb (Ab118776) with the ED50 of 4.75 ng/mL.
46.6 kDa, under reducing conditions; 46.6 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM Tris pH 7.5, 0.1M KCl, 5mM MgSO4.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human NSE Protein (rp169606) - ELISA Measured by its binding ability in a functional ELISA. Immobilized human NSE (rp169606) at 1.0 μg/mL can bind NSE Mouse mAb (Ab118776) with the ED₅₀ of 4.75 ng/mL.
Recombinant Human NSE Protein (rp169606) - SDS-PAGE 3 μg/lane of Recombinant Human NSE Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 46.6 kDa.
ZJ24F0303259
危险品化学品经营许可证（带存储）