2.5 Units/μg Unit definition:One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 95% completion at 4°C in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, pH 7.0.
Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description: The HRV 3C Protease is a recombinant cysteine protease from human rhinovirus 3C (HRV 3C)expressed in and purified from Escherichia coli. HRV 3C Protease cleaves protein substrates with the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro between the Gln and Gly residues. The high specificity and affinity tags( 6xHis) of the protease make it an ideal choice for the removal of purification and detection tags on recombinant proteins and allows for flexibility in protease removal. Source: HRV 3C Protease is a recombinant cysteine protease from human rhinovirus 3C (HRV 3C) expressed in and purified from Escherichia coli. HRV 3C enzyme digestion of His-GST-IL33 protein, according to the mass ratio (HRV 3C: target protein) 1:25 and 1:50 enzyme digestion, overnight at 4℃ enzyme digestion results are as follows: completely clean enzyme digestion
2.5 Units/μg Unit definition:One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 95% completion at 4°C in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, pH 7.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存12个月; 收货后建议分装 , 避免反复冻融。
图片
Recombinant HRV 3C Protease Protein (rp170396) - SDS-PAGE 3 μg/lane of Recombinant HRV 3C Protease Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 24.0 kDa.