CD3 epsilon (T-Cell Surface Glycoprotein CD3 Epsilon) is a type I transmembrane protein that belongs to the Ig (Immunoglobulin) superfamily. Human CD3 epsilon consists of 104 amino acid (aa) extracellular domain, 26 aa transmembrane domain and 55 aa cytoplasmic domain. With the extracellular domain, human CD3 epsilon shares 44.2% and 49% homology with mouse and rat CD3 epsilon respectively. There are four CD3 proteins: CD3δ, CD3 epsilon, CD3 gamma, and CD3 zeta. All CD3 proteins contain ITAM (Immunoreceptor Tyrosine-based Activation Motifs) in the cytoplasmic tail, which becomes phosphorylated by Src family protein tyrosine kinases LCK and FYN upon TCR engagement. CD3 proteins form heterodimers of CD3δ/CD3 epsilon and CD3 gamma /CD3 epsilon, which bind to TCR and form trimeric TCR alpha /CD3 epsilon /CD3 gamma and TCR beta /CD3 gamma /CD3 epsilon. The resulting heterohexamer further associates with CD3 zeta homodimer and forms TCR/CD3 signaling complex. Similar complex is also formed with TCR gamma and TCRδ. CD3 epsilon plays essential role in adaptive immune response. Mutations in CD3 epsilon lead to SCID (severe combined immunodeficiency).
Post-translational: Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
生物活性
Immobilized Recombinant Human CD3 epsilon Protein (rp175910) at 1.0 μg/mL can bind Epcoritamab (anti-CD3&CD20) (Ab175531) with the EC50 of 39.7 ng/mL.
13.5-16.0 kDa, under reducing conditions; 15.1 & 26.8 & 42.9 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human CD3 epsilon Protein (rp175910) - ELISA Immobilized Recombinant Human CD3 epsilon Protein (rp175910) at 1.0 μg/mL can bind Epcoritamab (anti-CD3&CD20) (Ab175531) with the EC₅₀ of 39.7 ng/mL.
Recombinant Human CD3 epsilon Protein (rp175910) - SDS-PAGE 3 μg/lane of Recombinant Human CD3 epsilon Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 13.5-16.0 kDa under reducing conditions and 15.1 & 26.8 & 42.9 kDa under non-reducing conditions. CD3 epsilon partially forms trimer and dimer under non-reducing conditions.