Interleukin 5 | BCDF mu | BCGFII | EDF | Eo-CSF | IL5 | TRF | Interleukin-5 | IL-5 | B-cell differentiation factor I | Eosinophil differentiation factor | T-cell replacing factor | BCDFII | B cell differentiation factor I
规格或纯度
无动物源,无载体,生物活性,ActiBioPure™,高性能,His标签,≥95%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:2653458, PubMed:9010276).
Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity).
Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed:1495999, PubMed:22528658).
Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed:7613138).
生物活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.22ng/mL.
16.2-21.3 kDa; under reducing conditions; 30.0-40.5 kDa; under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human IL-5 Protein (rp181291) - Protein Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.22 ng/mL.
Recombinant Human IL-5 Protein (rp181291) - SDS-PAGE
3 μg/lane of Recombinant Human IL-5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.2-21.3 kDa under reducing conditions and 30.0-40.5 kDa under non-reducing conditions.