Immobilized Recombinant Human CD27 Ligand/TNFSF7 Protein (rp181559) at 3.0 μg/mL can bind Cusatuzumab (anti-CD70) (Ab170844) with the EC50 of 188.8 ng/mL.
CD70, a member of the tumor necrosis factor superfamily, is restricted to activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor, CD27, is important in priming, effector functions, differentiation and memory formation of T-cells as well as plasma and memory B-cell generation. Tight control of CD70 expression is required to prevent lethal immunodeficiency. By selective transcription, CD70 is largely confined to activated lymphocytes and dendritic cells (DC). As a type II transmembrane receptor, CD70 is normally expressed on a subset of B, T and NK cells, where it plays a costimulatory role in immune cell activation. Immunohistochemical analysis of CD70 expression in multiple carcinoma types. The restricted expression pattern of CD70 in normal tissues and its widespread expression in various malignancies makes it an attractive target for antibody-based therapeutics. Investigations to exploit CD70 as a cancer target have lead to the identification of potential antibody-based clinical candidates.
Post-translational: N-glycosylated.
生物活性
Immobilized Recombinant Human CD27 Ligand/TNFSF7 Protein (rp181559) at 3.0 μg/mL can bind Cusatuzumab (anti-CD70) (Ab170844) with the EC50 of 188.8 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
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Recombinant Human CD27 Ligand/TNFSF7 Protein (rp181559) - ELISA
Immobilized Recombinant Human CD27 Ligand/TNFSF7 Protein (rp181559) at 3.0 μg/mL can bind Cusatuzumab (anti-CD70) (Ab170844) with the EC50 of 188.8 ng/mL.
Recombinant Human CD27 Ligand/TNFSF7 Protein (rp181559) - SDS-PAGE
3 μg/lane of Recombinant Human CD27 Ligand/TNFSF7 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 46.8-58.1 kDa under reducing conditions and 101.0 & 248.5 kDa under non-reducing conditions.
There are multiple pairs of disulfide bonds in the protein sequence. Disulfide bonds cause the protein to present as a polymer in a non-reducing conditions (Uniprot ID:P32970-1).