Recombinant Mouse Alpha-synuclein Protein

货号: rp183595
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 PBS Only ? 仅 PBS 配方 —— 以磷酸盐缓冲液供应,不含其他添加剂。当需要不含稳定剂/载体的洁净 PBS 缓冲液时使用。 ≥95%(SDS-PAGE)
Accession #
O55042
蛋白标签
C-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185363) with the EC50 of 19.8 ng/mL. Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185365) with the EC50 of 28.3 ng/mL. Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 5.0 μg/mL can bind Cinpanemab (anti-Alpha-synuclein) (Ab176594) with the EC50 of 475.3 ng/mL.
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规格
库存
价格
数量
10μg
rp183595-10μg
现货 Stock Image
¥269.90
50μg
rp183595-50μg
现货 Stock Image
¥799.90
100μg
rp183595-100μg
现货 Stock Image
¥1,269.90
1mg
rp183595-1mg
现货 Stock Image
¥6,469.90
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为什么选择此级别

ActiBioPure™,His标签,PBS Only,无载体,生物活性 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称
Recombinant Mouse Alpha-synuclein Protein
别名
重组小鼠Alpha-synuclein蛋白 | 重组小鼠α突触核蛋白
英文别名
alpha-Synuclein | I+/--synuclein | MGC110988 | NACP | non A-beta component of AD amyloid | Non-A beta component of AD amyloid | Non-A4 component of amyloid precursor | PARK1 | PARK4 | PD1 | SNCA | synuclein alpha-140 | synuclein, alpha (non A4 component o
规格或纯度
无载体, 生物活性, ActiBioPure™, His标签, PBS Only, ≥95%(SDS-PAGE)
生化机理
Alpha-Synuclein (alpha-Syn), also known as NACP or SNCA, exists as at least two structural isoforms: one is helix-rich, membrane-bound form that both the N- and C-terminal regions of alpha-synuclein are tightly associated with membranes and the other is disordered, cytosolic form. Synuclein is found predominantly in the presynaptic termini, in both free or membrane-bound forms. SNCA is extensively localized in nucleus of neurons. It has been shown that alpha-Synuclein was highly expressed in the mitochondria in olfactory bulb, hippocampus, striatum, and thalamus, where the cytosolic alpha-Synuclein was also rich. Normally the unstructured soluble type of alpha-synuclein can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy bodies and multiple system atrophy. SNCA abnormality and mitochondrial deficiency are two major changes in the brain of patients with Parkinson's disease (PD). Besides, alpha-synuclein is an abundant component of Lewy bodies in sporadic Parkinson's disease and diffuse Lewy body disease.  Post-translational: Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress. Ubiquitinated. The predominant conjugate is the diubiquitinated form. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. 
生物活性
Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185363) with the EC50 of 19.8 ng/mL. Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185365) with the EC50 of 28.3 ng/mL. Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 5.0 μg/mL can bind Cinpanemab (anti-Alpha-synuclein) (Ab176594) with the EC50 of 475.3 ng/mL.
表达系统
E. coli
种属
小鼠(Mouse)
氨基酸
1-140 aa
序列
MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEAHHHHHH
蛋白标签
C-His
无载体
Yes
无动物源
No
来源
重组表达
预测分子量
15.3 kDa
SDS-PAGE
17.1 kDa, under reducing conditions; 17.1 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片

Recombinant Mouse Alpha-synuclein Protein (rp183595) - ELISA
Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185363) with the EC₅₀ of 19.8 ng/mL.

Recombinant Mouse Alpha-synuclein Protein (rp183595) - SDS-PAGE
2 μg/lane of Recombinant Mouse Alpha-synuclein Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 17.1 kDa.

Recombinant Mouse Alpha-synuclein Protein (rp183595) - ELISA
Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185365) with the EC₅₀ of 28.3 ng/mL.

Recombinant Mouse Alpha-synuclein Protein (rp183595) - ELISA
Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 5.0 μg/mL can bind Cinpanemab (anti-Alpha-synuclein) (Ab176594) with the EC₅₀ of 475.3 ng/mL.

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找到8个结果

批号(Lot Number) 证书类型 货号
ZJ24F1215801 分析证书 rp183595
ZJ24F1215802 分析证书 rp183595
ZJ24F1215803 分析证书 rp183595
ZJ24F1215804 分析证书 rp183595
ZJ25F0521759 分析证书 rp183595
ZJ25F0521760 分析证书 rp183595
ZJ25F0521761 分析证书 rp183595
ZJ25F0521762 分析证书 rp183595
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