Immobilized Recombinant Human Growth Hormone Protein (rp181573) at 2.0 μg/mL can bind Recombinant Human Growth Hormone R (GHR) Protein (rp183621) with the EC50 of 55.1 ng/mL.
GH receptor | GHBP | GHR | growth hormone binding protein | Growth Hormone R | growth hormone receptor | serum binding protein | Somatotropin receptor
规格或纯度
无动物源,无载体,生物活性,ActiBioPure™,His标签,PBS Only,≥95%(SDS-PAGE),See COA
生化机理
Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.Post-translational: The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE (PubMed:11785980).Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17 (By similarity).On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2.3 publications Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome(PubMed:21980433, PubMed:25505247, PubMed:31182716, PubMed:34857742).Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of growth hormone receptor signaling (PubMed:21980433). Ubiquitination is not sufficient for GHR internalization (By similarity).
生物活性
Immobilized Recombinant Human Growth Hormone Protein (rp181573) at 2.0 μg/mL can bind Recombinant Human Growth Hormone R (GHR) Protein (rp183621) with the EC50 of 55.1 ng/mL.
33.3-45.8 kDa, under reducing conditions; 33.5-47.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
See COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human Growth Hormone R (GHR) Protein (rp183621) - Binding Activity
Immobilized Recombinant Human Growth Hormone Protein (rp181573) at 2.0 μg/mL can bind Recombinant Human Growth Hormone R (GHR) Protein (rp183621) with the EC₅₀ of 55.1 ng/mL.
Recombinant Human Growth Hormone R (GHR) Protein (rp183621) - SDS-PAGE
3 μg/lane of Recombinant Human Growth Hormone R (GHR) Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 33.3-45.8 kDa under reducing conditions and 33.5-47.0 kDa under non-reducing conditions.
ZJ25F1129686
危险品化学品经营许可证（带存储）