Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Red blood cells, added at 10 x 106 cells/mL (100 µL/well) to SIRP alpha coated plates, will adhere after 1 hour at 37° C. The ED50 for this effect is1.5 µg/mL. Immobilized Recombinant Human SIRP alpha/CD172a Protein (rp183642) at 1.0 μg/mL can bind Recombinant Human CD47 Protein (rp144028) with the EC50 of 829.1 ng/mL. Immobilized Recombinant Human SIRP alpha/CD172a Protein (rp183642) at 2.0 μg/mL can bind Recombinant Human CD47 Protein (rp169585) with the EC50 of 308.6 ng/mL.
Signal regulatory protein alpha (SIRP alpha, designated CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), is a monomeric ~90 kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily. SIRPs are paired receptors, with similar extracellular domains but differing C-termini and functions. The 503 amino acid (aa) human SIRP alpha contains a 342 aa extracellular domain (ECD), with one V-type, and two C1 type Ig domains, and three potential N glycosylation sites. It has a 110 aa cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated. Human SIRP alpha has more than 40 described polymorphisms, including the prominent BIT (Brain Ig like molecule with Tyrosine-based activation motifs, also called SIRP alpha 2 or PTPNS). One reported isoform lacks aa 1-101, which eliminates most of the V type Ig domain. Human SIRP alpha ECD shares 61%, 60%, 71%, 72% and 73% aa identity with mouse, rat, porcine, bovine and equine SIRP alpha, respectively; it shares 84% and 76% aa identity with human SIRP beta 1 and SIRP gamma, respectively. SIRP alpha is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It is also found on neurons, smooth muscle and endothelial cells. SIRP alpha shows adhesion to the ubiquitous CD47/IAP (integrin associated protein), while SIRP gamma binds more weakly and SIRP alpha 1 does not bind at all. Mouse and human SIRP alpha -CD47 binding only cross-reacts for specific polymorphisms and influences engraftment of xenotransplanted stem cells. SIRP alpha engagement generally produces a negative regulatory signal. Low SIRP alpha recognition of CD47, which occurs on aged erythrocytes or platelets or xenogenic cells, promotes clearance of CD47low cells from circulation. SIRP alpha recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production. The CD47 integrin-SIRP alpha interaction is reported to promote macrophage fusion during osteoclastogenesis.
Post-translational: N-glycosylated. Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
生物活性
Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Red blood cells, added at 10 x 106 cells/mL (100 µL/well) to SIRP alpha coated plates, will adhere after 1 hour at 37° C. The ED50 for this effect is1.5 µg/mL. Immobilized Recombinant Human SIRP alpha/CD172a Protein (rp183642) at 1.0 μg/mL can bind Recombinant Human CD47 Protein (rp144028) with the EC50 of 829.1 ng/mL. Immobilized Recombinant Human SIRP alpha/CD172a Protein (rp183642) at 2.0 μg/mL can bind Recombinant Human CD47 Protein (rp169585) with the EC50 of 308.6 ng/mL.
47.4-56.4 kDa, under reducing conditions; 46.1-53.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human SIRP alpha/CD172a Protein (rp183642) - Binding Activity Immobilized Recombinant Human SIRP alpha/CD172a Protein (rp183642) at 1.0 μg/mL can bind Recombinant Human CD47 Protein (rp144028) with the EC₅₀ of 829.1 ng/mL.
Recombinant Human SIRP alpha/CD172a Protein (rp183642) - SDS-PAGE 3 μg/lane of Recombinant Human SIRP alpha/CD172a Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 47.4-56.4 kDa under reducing conditions and 46.1-53.4 kDa under non-reducing conditions.
Recombinant Human SIRP alpha/CD172a Protein (rp183642) - Bioactivity Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Red blood cells, added at 10 x 106 cells/mL (100 µL/well) to SIRP alpha coated plates, will adhere after 1 hour at 37° C. The ED50 for this effect is 1.5 µg/mL.
Recombinant Human SIRP alpha/CD172a Protein - Binding Activity Immobilized Recombinant Human SIRP alpha/CD172a Protein (rp183642) at 2.0 μg/mL can bind Recombinant Human CD47 Protein (rp169585) with the EC50 of 308.6 ng/mL.
ZJ24F1216155
危险品化学品经营许可证（带存储）