Measured by its ability to inhibit IL-4 induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 42.55 ng/mL in the presence of 20.0 ng/mL of Recombinant Human IL-4 Protein (rp147593).
Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
Post-translational: On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
生物活性
Measured by its ability to inhibit IL-4 induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 42.55 ng/mL in the presence of 20.0 ng/mL of Recombinant Human IL-4 Protein (rp147593).
38.1-53.0 kDa, under reducing conditions; 38.1-53.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human IL-4R alpha Protein (rp184044) - Protein Bioactivity Measured by its ability to inhibit IL-4 induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 42.55 ng/mL in the presence of 20.0 ng/mL of Recombinant Human IL-4 Protein (rp147593).
Recombinant Human IL-4R alpha Protein (rp184044) - SDS-PAGE 3 μg/lane of Recombinant Human IL-4R alpha Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 38.1-53.0 kDa.
ZJ24F1113643
危险品化学品经营许可证（带存储）