Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. Immobilized Recombinant Human IL-12 protein (rp156174) at 1.0 μg/mL can bind Ebdarokimab (anti-IL-12B) (Ab182919) with the EC50 of 9.68 ng/mL.
Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description: IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
规格
产品名称
Recombinant Human IL-12 Protein
别名
重组人IL-12蛋白 | 重组人 IL-12 蛋白
英文别名
CLMF Protein | CLMF2 Protein | IL-12B Protein | NKSF Protein | NKSF2 Protein | p40 Protein | Il12p40 Protein | Il-12p40 Protein | Il12 Protein | IL-12 Protein | LOC100217488 Protein | IL-12p35 Protein | Il-12a Protein | Ll12a Protein | p35 Protein | IL12B
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. Immobilized Recombinant Human IL-12 protein (rp156174) at 1.0 μg/mL can bind Ebdarokimab (anti-IL-12B) (Ab182919) with the EC50 of 9.68 ng/mL.
35-45 kDa, under reducing conditions; 28-40 & 55-69 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human IL-12 Protein (rp184356) - Protein Bioactivity Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED₅₀ for this effect is 0.01-0.05 ng/mL.
Recombinant Human IL-12 Protein (rp184356) - SDS-PAGE 3 μg/lane of Recombinant Human IL-12 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 35-45 kDa under reducing conditions and 28-42 & 45-70 kDa under non-reducing conditions.
Recombinant Human IL-12 Protein (rp184356) - ELISA Immobilized Recombinant Human IL-12 protein (rp156174) at 1.0 μg/mL can bind Ebdarokimab (anti-IL-12B) (Ab182919) with the EC₅₀ of 9.68 ng/mL.