Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description: IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
规格
产品名称
Recombinant Human IL-12 Protein
别名
重组人IL12蛋白 | 重组人 IL-12 蛋白
英文别名
CLMF Protein | CLMF2 Protein | IL-12B Protein | NKSF Protein | NKSF2 Protein | p40 Protein | Il12p40 Protein | Il-12p40 Protein | Il12 Protein | IL-12 Protein | LOC100217488 Protein | IL-12p35 Protein | Il-12a Protein | Ll12a Protein | p35 Protein | IL12B
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human IL-12 Protein (rp156174) - Protein Bioactivity Recombinant human IL-12 bioactivity is measured in a cell proliferation assay using PHA-P-activated human peripheral blood mononuclear cell (PBMC), the ED₅₀ for this effect is less than 0.04 ng/mL.
Recombinant human IL-12 protein (rp156174) - SDS-PAGE Recombinant human IL-12 protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 40.6 kDa.