级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。高性能?高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥95%(SDS-PAGE)expressed in CHO, 见 COA
Accession #
Q99N32
蛋白标签
C-10His
表达系统
CHO
内毒素水平
<1.0 EU/μg
生物活性
Recombinant Mouse Klotho beta Protein (rp184968) cooperates with FGF21 to promote NIH-3T3 cell proliferation.The ED50 for this effect is 0.53 μg/mL in the presence of Recombinant mouse FGF21.
Immobilized Recombinant Human FGF19 Protein (rp145934) at 2.0 μg/mL can bind Recombinant Mouse Klotho beta Protein (rp184968) with the EC50 of 153.2 ng/mL.
Immobilized Recombinant Mouse Klotho beta Protein (rp184968) at 2.0 μg/mL can bind Recombinant Human FGF19 Protein (rp145934) with the EC50 of 1.25 μg/mL.
无动物源,无载体,生物活性,ActiBioPure™,高性能,His标签,≥95%(SDS-PAGE),expressed in CHO, 见 COA
生化机理
Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. Like Klotho, Klotho beta facilitates binding between FGF-19 subfamily members and their receptors via formation of a ternary complex. The Klotho beta mediated interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes, which allows FGF-21 to stimulate GLUT1 expression, up-regulating adipocyte insulin-dependent glucose uptake. The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes. However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible. The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.
生物活性
Recombinant Mouse Klotho beta Protein (rp184968) cooperates with FGF21 to promote NIH-3T3 cell proliferation.The ED50 for this effect is 0.53 μg/mL in the presence of Recombinant mouse FGF21.
Immobilized Recombinant Human FGF19 Protein (rp145934) at 2.0 μg/mL can bind Recombinant Mouse Klotho beta Protein (rp184968) with the EC50 of 153.2 ng/mL.
Immobilized Recombinant Mouse Klotho beta Protein (rp184968) at 2.0 μg/mL can bind Recombinant Human FGF19 Protein (rp145934) with the EC50 of 1.25 μg/mL.
124.9-150.0 kDa, under reducing conditions; 124.9-150.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in CHO, 见 COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 6个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Mouse Klotho beta Protein (rp184968) - Protein Bioactivity
Recombinant Mouse Klotho beta Protein (rp184968) cooperates with FGF21 to promote NIH-3T3 cell proliferation. The ED50 or this effect is 0.53 μg/mL in the presence of Recombinant mouse FGF21.
Recombinant Mouse Klotho beta Protein (rp184968) - Binding
Activity
Immobilized Recombinant Human FGF19 Protein (rp145934) at 2.0 μg/mL can bind Recombinant Mouse Klotho beta Protein (rp184968) with the EC50 of 153.2 ng/mL.
Recombinant Mouse Klotho beta Protein (rp184968) - Binding Activity
Immobilized Recombinant Mouse Klotho beta Protein (rp184968) at 2.0 μg/mL can bind Recombinant Human FGF19 Protein (rp145934) with the EC50 of 1.25 μg/mL.
Recombinant Mouse Klotho beta Protein (rp184968) - SDS-PAGE
3 μg/lane of Recombinant Mouse Klotho beta Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 124.9-159.5 kDa.
危险品化学品经营许可证（带存储）